Sickle Cell Trait Modulates the Proteome and Phosphoproteome of Plasmodium falciparum-Infected Erythrocytes
The high prevalence of sickle cell disease in some human populations likely results from the protection afforded against severe Plasmodium falciparum malaria and death by heterozygous carriage of HbS. P. falciparum remodels the erythrocyte membrane and skeleton, displaying parasite proteins at the e...
Main Authors: | , , , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2021-03-01
|
Series: | Frontiers in Cellular and Infection Microbiology |
Subjects: | |
Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2021.637604/full |
id |
doaj-832879f0770b47558930f1715e2b7709 |
---|---|
record_format |
Article |
spelling |
doaj-832879f0770b47558930f1715e2b77092021-03-24T05:56:41ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882021-03-011110.3389/fcimb.2021.637604637604Sickle Cell Trait Modulates the Proteome and Phosphoproteome of Plasmodium falciparum-Infected ErythrocytesMargaux Chauvet0Margaux Chauvet1Cerina Chhuon2Joanna Lipecka3Sébastien Dechavanne4Sébastien Dechavanne5Sébastien Dechavanne6Célia Dechavanne7Murielle Lohezic8Margherita Ortalli9Margherita Ortalli10Damien Pineau11Damien Pineau12Jean-Antoine Ribeil13Sandra Manceau14Sandra Manceau15Caroline Le Van Kim16Caroline Le Van Kim17Caroline Le Van Kim18Adrian J. F. Luty19Florence Migot-Nabias20Slim Azouzi21Slim Azouzi22Slim Azouzi23Ida Chiara Guerrera24Anaïs Merckx25Anaïs Merckx26Université de Paris, MERIT, IRD, Paris, FranceLaboratoire d’Excellence GR-Ex, Paris, FranceUniversité de Paris, Proteomics Platform Necker, Structure Fédérative de Recherche Necker, Inserm US24/CNRS, UMS3633, Paris, FranceUniversité de Paris, Proteomics Platform Necker, Structure Fédérative de Recherche Necker, Inserm US24/CNRS, UMS3633, Paris, FranceLaboratoire d’Excellence GR-Ex, Paris, FranceUniversité de Paris, Inserm, BIGR, Paris, FranceInstitut National de la Transfusion Sanguine, Paris, FranceUniversité de Paris, MERIT, IRD, Paris, FranceUniversité de Paris, MERIT, IRD, Paris, FranceUniversité de Paris, MERIT, IRD, Paris, FranceLaboratoire d’Excellence GR-Ex, Paris, FranceUniversité de Paris, MERIT, IRD, Paris, FranceLaboratoire d’Excellence GR-Ex, Paris, FranceBiotherapy Department, Necker Children’s Hospital, Assistance Publique-Hôpitaux de Paris, Paris, FranceLaboratoire d’Excellence GR-Ex, Paris, FranceBiotherapy Department, Necker Children’s Hospital, Assistance Publique-Hôpitaux de Paris, Paris, FranceLaboratoire d’Excellence GR-Ex, Paris, FranceUniversité de Paris, Inserm, BIGR, Paris, FranceInstitut National de la Transfusion Sanguine, Paris, FranceUniversité de Paris, MERIT, IRD, Paris, FranceUniversité de Paris, MERIT, IRD, Paris, FranceLaboratoire d’Excellence GR-Ex, Paris, FranceUniversité de Paris, Inserm, BIGR, Paris, FranceInstitut National de la Transfusion Sanguine, Paris, FranceUniversité de Paris, Proteomics Platform Necker, Structure Fédérative de Recherche Necker, Inserm US24/CNRS, UMS3633, Paris, FranceUniversité de Paris, MERIT, IRD, Paris, FranceLaboratoire d’Excellence GR-Ex, Paris, FranceThe high prevalence of sickle cell disease in some human populations likely results from the protection afforded against severe Plasmodium falciparum malaria and death by heterozygous carriage of HbS. P. falciparum remodels the erythrocyte membrane and skeleton, displaying parasite proteins at the erythrocyte surface that interact with key human proteins in the Ankyrin R and 4.1R complexes. Oxidative stress generated by HbS, as well as by parasite invasion, disrupts the kinase/phosphatase balance, potentially interfering with the molecular interactions between human and parasite proteins. HbS is known to be associated with abnormal membrane display of parasite antigens. Studying the proteome and the phosphoproteome of red cell membrane extracts from P. falciparum infected and non-infected erythrocytes, we show here that HbS heterozygous carriage, combined with infection, modulates the phosphorylation of erythrocyte membrane transporters and skeletal proteins as well as of parasite proteins. Our results highlight modifications of Ser-/Thr- and/or Tyr- phosphorylation in key human proteins, such as ankyrin, β-adducin, β-spectrin and Band 3, and key parasite proteins, such as RESA or MESA. Altered phosphorylation patterns could disturb the interactions within membrane protein complexes, affect nutrient uptake and the infected erythrocyte cytoadherence phenomenon, thus lessening the severity of malaria symptoms.https://www.frontiersin.org/articles/10.3389/fcimb.2021.637604/fullPlasmodium falciparumhemoglobin Serythrocytemembrane phosphorylationproteomics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Margaux Chauvet Margaux Chauvet Cerina Chhuon Joanna Lipecka Sébastien Dechavanne Sébastien Dechavanne Sébastien Dechavanne Célia Dechavanne Murielle Lohezic Margherita Ortalli Margherita Ortalli Damien Pineau Damien Pineau Jean-Antoine Ribeil Sandra Manceau Sandra Manceau Caroline Le Van Kim Caroline Le Van Kim Caroline Le Van Kim Adrian J. F. Luty Florence Migot-Nabias Slim Azouzi Slim Azouzi Slim Azouzi Ida Chiara Guerrera Anaïs Merckx Anaïs Merckx |
spellingShingle |
Margaux Chauvet Margaux Chauvet Cerina Chhuon Joanna Lipecka Sébastien Dechavanne Sébastien Dechavanne Sébastien Dechavanne Célia Dechavanne Murielle Lohezic Margherita Ortalli Margherita Ortalli Damien Pineau Damien Pineau Jean-Antoine Ribeil Sandra Manceau Sandra Manceau Caroline Le Van Kim Caroline Le Van Kim Caroline Le Van Kim Adrian J. F. Luty Florence Migot-Nabias Slim Azouzi Slim Azouzi Slim Azouzi Ida Chiara Guerrera Anaïs Merckx Anaïs Merckx Sickle Cell Trait Modulates the Proteome and Phosphoproteome of Plasmodium falciparum-Infected Erythrocytes Frontiers in Cellular and Infection Microbiology Plasmodium falciparum hemoglobin S erythrocyte membrane phosphorylation proteomics |
author_facet |
Margaux Chauvet Margaux Chauvet Cerina Chhuon Joanna Lipecka Sébastien Dechavanne Sébastien Dechavanne Sébastien Dechavanne Célia Dechavanne Murielle Lohezic Margherita Ortalli Margherita Ortalli Damien Pineau Damien Pineau Jean-Antoine Ribeil Sandra Manceau Sandra Manceau Caroline Le Van Kim Caroline Le Van Kim Caroline Le Van Kim Adrian J. F. Luty Florence Migot-Nabias Slim Azouzi Slim Azouzi Slim Azouzi Ida Chiara Guerrera Anaïs Merckx Anaïs Merckx |
author_sort |
Margaux Chauvet |
title |
Sickle Cell Trait Modulates the Proteome and Phosphoproteome of Plasmodium falciparum-Infected Erythrocytes |
title_short |
Sickle Cell Trait Modulates the Proteome and Phosphoproteome of Plasmodium falciparum-Infected Erythrocytes |
title_full |
Sickle Cell Trait Modulates the Proteome and Phosphoproteome of Plasmodium falciparum-Infected Erythrocytes |
title_fullStr |
Sickle Cell Trait Modulates the Proteome and Phosphoproteome of Plasmodium falciparum-Infected Erythrocytes |
title_full_unstemmed |
Sickle Cell Trait Modulates the Proteome and Phosphoproteome of Plasmodium falciparum-Infected Erythrocytes |
title_sort |
sickle cell trait modulates the proteome and phosphoproteome of plasmodium falciparum-infected erythrocytes |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cellular and Infection Microbiology |
issn |
2235-2988 |
publishDate |
2021-03-01 |
description |
The high prevalence of sickle cell disease in some human populations likely results from the protection afforded against severe Plasmodium falciparum malaria and death by heterozygous carriage of HbS. P. falciparum remodels the erythrocyte membrane and skeleton, displaying parasite proteins at the erythrocyte surface that interact with key human proteins in the Ankyrin R and 4.1R complexes. Oxidative stress generated by HbS, as well as by parasite invasion, disrupts the kinase/phosphatase balance, potentially interfering with the molecular interactions between human and parasite proteins. HbS is known to be associated with abnormal membrane display of parasite antigens. Studying the proteome and the phosphoproteome of red cell membrane extracts from P. falciparum infected and non-infected erythrocytes, we show here that HbS heterozygous carriage, combined with infection, modulates the phosphorylation of erythrocyte membrane transporters and skeletal proteins as well as of parasite proteins. Our results highlight modifications of Ser-/Thr- and/or Tyr- phosphorylation in key human proteins, such as ankyrin, β-adducin, β-spectrin and Band 3, and key parasite proteins, such as RESA or MESA. Altered phosphorylation patterns could disturb the interactions within membrane protein complexes, affect nutrient uptake and the infected erythrocyte cytoadherence phenomenon, thus lessening the severity of malaria symptoms. |
topic |
Plasmodium falciparum hemoglobin S erythrocyte membrane phosphorylation proteomics |
url |
https://www.frontiersin.org/articles/10.3389/fcimb.2021.637604/full |
work_keys_str_mv |
AT margauxchauvet sicklecelltraitmodulatestheproteomeandphosphoproteomeofplasmodiumfalciparuminfectederythrocytes AT margauxchauvet sicklecelltraitmodulatestheproteomeandphosphoproteomeofplasmodiumfalciparuminfectederythrocytes AT cerinachhuon sicklecelltraitmodulatestheproteomeandphosphoproteomeofplasmodiumfalciparuminfectederythrocytes AT joannalipecka sicklecelltraitmodulatestheproteomeandphosphoproteomeofplasmodiumfalciparuminfectederythrocytes AT sebastiendechavanne sicklecelltraitmodulatestheproteomeandphosphoproteomeofplasmodiumfalciparuminfectederythrocytes AT sebastiendechavanne sicklecelltraitmodulatestheproteomeandphosphoproteomeofplasmodiumfalciparuminfectederythrocytes AT sebastiendechavanne sicklecelltraitmodulatestheproteomeandphosphoproteomeofplasmodiumfalciparuminfectederythrocytes AT celiadechavanne sicklecelltraitmodulatestheproteomeandphosphoproteomeofplasmodiumfalciparuminfectederythrocytes AT muriellelohezic sicklecelltraitmodulatestheproteomeandphosphoproteomeofplasmodiumfalciparuminfectederythrocytes AT margheritaortalli sicklecelltraitmodulatestheproteomeandphosphoproteomeofplasmodiumfalciparuminfectederythrocytes AT margheritaortalli sicklecelltraitmodulatestheproteomeandphosphoproteomeofplasmodiumfalciparuminfectederythrocytes AT damienpineau sicklecelltraitmodulatestheproteomeandphosphoproteomeofplasmodiumfalciparuminfectederythrocytes AT damienpineau sicklecelltraitmodulatestheproteomeandphosphoproteomeofplasmodiumfalciparuminfectederythrocytes AT jeanantoineribeil sicklecelltraitmodulatestheproteomeandphosphoproteomeofplasmodiumfalciparuminfectederythrocytes AT sandramanceau sicklecelltraitmodulatestheproteomeandphosphoproteomeofplasmodiumfalciparuminfectederythrocytes AT sandramanceau sicklecelltraitmodulatestheproteomeandphosphoproteomeofplasmodiumfalciparuminfectederythrocytes AT carolinelevankim sicklecelltraitmodulatestheproteomeandphosphoproteomeofplasmodiumfalciparuminfectederythrocytes AT carolinelevankim sicklecelltraitmodulatestheproteomeandphosphoproteomeofplasmodiumfalciparuminfectederythrocytes AT carolinelevankim sicklecelltraitmodulatestheproteomeandphosphoproteomeofplasmodiumfalciparuminfectederythrocytes AT adrianjfluty sicklecelltraitmodulatestheproteomeandphosphoproteomeofplasmodiumfalciparuminfectederythrocytes AT florencemigotnabias sicklecelltraitmodulatestheproteomeandphosphoproteomeofplasmodiumfalciparuminfectederythrocytes AT slimazouzi sicklecelltraitmodulatestheproteomeandphosphoproteomeofplasmodiumfalciparuminfectederythrocytes AT slimazouzi sicklecelltraitmodulatestheproteomeandphosphoproteomeofplasmodiumfalciparuminfectederythrocytes AT slimazouzi sicklecelltraitmodulatestheproteomeandphosphoproteomeofplasmodiumfalciparuminfectederythrocytes AT idachiaraguerrera sicklecelltraitmodulatestheproteomeandphosphoproteomeofplasmodiumfalciparuminfectederythrocytes AT anaismerckx sicklecelltraitmodulatestheproteomeandphosphoproteomeofplasmodiumfalciparuminfectederythrocytes AT anaismerckx sicklecelltraitmodulatestheproteomeandphosphoproteomeofplasmodiumfalciparuminfectederythrocytes |
_version_ |
1724205269671477248 |