Xeno-Hybrid Bone Graft Releasing Biomimetic Proteins Promotes Osteogenic Differentiation of hMSCs
Bone defect is a noteworthy health problem and is the second most transplanted tissue after blood. Numerous bone grafts are designed and applied in clinics. Limitations, however, from different aspects still exist, including limited supply, mechanical strength, and bioactivity. In this study, two bi...
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doaj-8338d4142c2844ceaef3dfe922d3803d2020-12-22T13:42:56ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2020-12-01810.3389/fcell.2020.619111619111Xeno-Hybrid Bone Graft Releasing Biomimetic Proteins Promotes Osteogenic Differentiation of hMSCsHao Zhu0Veronika Hefka Blahnová1Veronika Hefka Blahnová2Giuseppe Perale3Giuseppe Perale4Giuseppe Perale5Jun Xiao6Felice Betge7Fabio Boniolo8Eva Filová9Eva Filová10Ståle Petter Lyngstadaas11Ståle Petter Lyngstadaas12Håvard Jostein Haugen13Håvard Jostein Haugen14Department of Orthopedic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Tissue Engineering, Institute of Experimental Medicine of the Czech Academy of Sciences, Prague, CzechiaDepartment of Biophysics, Second Faculty of Medicine, Charles University, Prague, CzechiaIndustrie Biomediche Insubri S.A., Mezzovico-Vira, SwitzerlandLudwig Boltzmann Institute for Experimental and Clinical Traumatology, Vienna, AustriaFaculty of Biomedical Sciences, University of Southern Switzerland, Lugano, SwitzerlandDepartment of Orthopedic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaIndustrie Biomediche Insubri S.A., Mezzovico-Vira, SwitzerlandHelmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH), Neuherberg, GermanyDepartment of Tissue Engineering, Institute of Experimental Medicine of the Czech Academy of Sciences, Prague, CzechiaDepartment of Biophysics, Second Faculty of Medicine, Charles University, Prague, CzechiaCorticalis AS, Oslo, NorwayDepartment of Biomaterials, Faculty of Dentistry, University of Oslo, Oslo, NorwayCorticalis AS, Oslo, NorwayDepartment of Biomaterials, Faculty of Dentistry, University of Oslo, Oslo, NorwayBone defect is a noteworthy health problem and is the second most transplanted tissue after blood. Numerous bone grafts are designed and applied in clinics. Limitations, however, from different aspects still exist, including limited supply, mechanical strength, and bioactivity. In this study, two biomimetic peptides (P2 and P6) are incorporated into a composite bioactive xeno hybrid bone graft named SmartBonePep®, with the aim to increase the bioactivity of the bone graft. The results, which include cytotoxicity, proliferation rate, confocal microscopy, gene expression, and protein qualification, successfully prove that the SmartBonePep® has multi-modal biological effects on human mesenchymal stem cells from bone marrow. The effective physical entrapment of P6 into a composite xeno-hybrid bone graft, withstanding manufacturing processes including exposure to strong organic solvents and ethylene oxide sterilization, increases the osteogenic potential of the stem cells as well as cell attachment and proliferation. P2 and P6 both show a strong biological potential and may be future candidates for enhancing the clinical performance of bone grafts.https://www.frontiersin.org/articles/10.3389/fcell.2020.619111/fullbone scaffoldbone graftbone regeneration biomimeticbioactive proteinsintrinsically disorderedmesenchymal stem cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hao Zhu Veronika Hefka Blahnová Veronika Hefka Blahnová Giuseppe Perale Giuseppe Perale Giuseppe Perale Jun Xiao Felice Betge Fabio Boniolo Eva Filová Eva Filová Ståle Petter Lyngstadaas Ståle Petter Lyngstadaas Håvard Jostein Haugen Håvard Jostein Haugen |
spellingShingle |
Hao Zhu Veronika Hefka Blahnová Veronika Hefka Blahnová Giuseppe Perale Giuseppe Perale Giuseppe Perale Jun Xiao Felice Betge Fabio Boniolo Eva Filová Eva Filová Ståle Petter Lyngstadaas Ståle Petter Lyngstadaas Håvard Jostein Haugen Håvard Jostein Haugen Xeno-Hybrid Bone Graft Releasing Biomimetic Proteins Promotes Osteogenic Differentiation of hMSCs Frontiers in Cell and Developmental Biology bone scaffold bone graft bone regeneration biomimetic bioactive proteins intrinsically disordered mesenchymal stem cells |
author_facet |
Hao Zhu Veronika Hefka Blahnová Veronika Hefka Blahnová Giuseppe Perale Giuseppe Perale Giuseppe Perale Jun Xiao Felice Betge Fabio Boniolo Eva Filová Eva Filová Ståle Petter Lyngstadaas Ståle Petter Lyngstadaas Håvard Jostein Haugen Håvard Jostein Haugen |
author_sort |
Hao Zhu |
title |
Xeno-Hybrid Bone Graft Releasing Biomimetic Proteins Promotes Osteogenic Differentiation of hMSCs |
title_short |
Xeno-Hybrid Bone Graft Releasing Biomimetic Proteins Promotes Osteogenic Differentiation of hMSCs |
title_full |
Xeno-Hybrid Bone Graft Releasing Biomimetic Proteins Promotes Osteogenic Differentiation of hMSCs |
title_fullStr |
Xeno-Hybrid Bone Graft Releasing Biomimetic Proteins Promotes Osteogenic Differentiation of hMSCs |
title_full_unstemmed |
Xeno-Hybrid Bone Graft Releasing Biomimetic Proteins Promotes Osteogenic Differentiation of hMSCs |
title_sort |
xeno-hybrid bone graft releasing biomimetic proteins promotes osteogenic differentiation of hmscs |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cell and Developmental Biology |
issn |
2296-634X |
publishDate |
2020-12-01 |
description |
Bone defect is a noteworthy health problem and is the second most transplanted tissue after blood. Numerous bone grafts are designed and applied in clinics. Limitations, however, from different aspects still exist, including limited supply, mechanical strength, and bioactivity. In this study, two biomimetic peptides (P2 and P6) are incorporated into a composite bioactive xeno hybrid bone graft named SmartBonePep®, with the aim to increase the bioactivity of the bone graft. The results, which include cytotoxicity, proliferation rate, confocal microscopy, gene expression, and protein qualification, successfully prove that the SmartBonePep® has multi-modal biological effects on human mesenchymal stem cells from bone marrow. The effective physical entrapment of P6 into a composite xeno-hybrid bone graft, withstanding manufacturing processes including exposure to strong organic solvents and ethylene oxide sterilization, increases the osteogenic potential of the stem cells as well as cell attachment and proliferation. P2 and P6 both show a strong biological potential and may be future candidates for enhancing the clinical performance of bone grafts. |
topic |
bone scaffold bone graft bone regeneration biomimetic bioactive proteins intrinsically disordered mesenchymal stem cells |
url |
https://www.frontiersin.org/articles/10.3389/fcell.2020.619111/full |
work_keys_str_mv |
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