Single-Cell Transcriptome Analysis Maps the Developmental Track of the Human Heart
Summary: The heart is the central organ of the circulatory system, and its proper development is vital for maintaining human life. Here, we used single-cell RNA sequencing to profile the gene expression landscapes of ∼4,000 cardiac cells from human embryos and identified four major types of cells: c...
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Format: | Article |
Language: | English |
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Elsevier
2019-02-01
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Series: | Cell Reports |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124719301081 |
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doaj-836a7c9bab4140fa8cc8cc9c64c0439c |
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record_format |
Article |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yueli Cui Yuxuan Zheng Xixi Liu Liying Yan Xiaoying Fan Jun Yong Yuqiong Hu Ji Dong Qingqing Li Xinglong Wu Shuai Gao Jingyun Li Lu Wen Jie Qiao Fuchou Tang |
spellingShingle |
Yueli Cui Yuxuan Zheng Xixi Liu Liying Yan Xiaoying Fan Jun Yong Yuqiong Hu Ji Dong Qingqing Li Xinglong Wu Shuai Gao Jingyun Li Lu Wen Jie Qiao Fuchou Tang Single-Cell Transcriptome Analysis Maps the Developmental Track of the Human Heart Cell Reports |
author_facet |
Yueli Cui Yuxuan Zheng Xixi Liu Liying Yan Xiaoying Fan Jun Yong Yuqiong Hu Ji Dong Qingqing Li Xinglong Wu Shuai Gao Jingyun Li Lu Wen Jie Qiao Fuchou Tang |
author_sort |
Yueli Cui |
title |
Single-Cell Transcriptome Analysis Maps the Developmental Track of the Human Heart |
title_short |
Single-Cell Transcriptome Analysis Maps the Developmental Track of the Human Heart |
title_full |
Single-Cell Transcriptome Analysis Maps the Developmental Track of the Human Heart |
title_fullStr |
Single-Cell Transcriptome Analysis Maps the Developmental Track of the Human Heart |
title_full_unstemmed |
Single-Cell Transcriptome Analysis Maps the Developmental Track of the Human Heart |
title_sort |
single-cell transcriptome analysis maps the developmental track of the human heart |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2019-02-01 |
description |
Summary: The heart is the central organ of the circulatory system, and its proper development is vital for maintaining human life. Here, we used single-cell RNA sequencing to profile the gene expression landscapes of ∼4,000 cardiac cells from human embryos and identified four major types of cells: cardiomyocytes (CMs), cardiac fibroblasts, endothelial cells (ECs), and valvar interstitial cells (VICs). Atrial and ventricular CMs acquired distinct features early in heart development. Furthermore, both CMs and fibroblasts show stepwise changes in gene expression. As development proceeds, VICs may be involved in the remodeling phase, and ECs display location-specific characteristics. Finally, we compared gene expression profiles between humans and mice and identified a series of unique features of human heart development. Our study lays the groundwork for elucidating the mechanisms of in vivo human cardiac development and provides potential clues to understand cardiac regeneration. : Cui et al. reveal the transcriptional landscapes of human fetal heart development at single-cell resolution and identify critical biological features of different cell types, providing insights into the molecular mechanisms of human heart development. Keywords: human fetal heart development, single-cell RNA-seq, cardiomyocytes, cardiac fibroblasts, endothelial cells, valvar interstitial cells, extracellular matrix, cross-species comparison, single-cell transcriptome analysis, human embryonic development |
url |
http://www.sciencedirect.com/science/article/pii/S2211124719301081 |
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doaj-836a7c9bab4140fa8cc8cc9c64c0439c2020-11-25T01:33:31ZengElsevierCell Reports2211-12472019-02-0126719341950.e5Single-Cell Transcriptome Analysis Maps the Developmental Track of the Human HeartYueli Cui0Yuxuan Zheng1Xixi Liu2Liying Yan3Xiaoying Fan4Jun Yong5Yuqiong Hu6Ji Dong7Qingqing Li8Xinglong Wu9Shuai Gao10Jingyun Li11Lu Wen12Jie Qiao13Fuchou Tang14Department of Obstetrics and Gynecology, Beijing Advanced Innovation Center for Genomics, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, China; Biomedical Institute for Pioneering Investigation via Convergence, Ministry of Education Key Laboratory of Cell Proliferation and Differentiation, Beijing 100871, ChinaDepartment of Obstetrics and Gynecology, Beijing Advanced Innovation Center for Genomics, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, China; Biomedical Institute for Pioneering Investigation via Convergence, Ministry of Education Key Laboratory of Cell Proliferation and Differentiation, Beijing 100871, China; Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, ChinaDepartment of Obstetrics and Gynecology, Beijing Advanced Innovation Center for Genomics, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, China; Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, China; Biomedical Institute for Pioneering Investigation via Convergence, Ministry of Education Key Laboratory of Cell Proliferation and Differentiation, Beijing 100871, China; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing 100191, ChinaDepartment of Obstetrics and Gynecology, Beijing Advanced Innovation Center for Genomics, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, China; Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, China; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing 100191, ChinaDepartment of Obstetrics and Gynecology, Beijing Advanced Innovation Center for Genomics, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, China; Biomedical Institute for Pioneering Investigation via Convergence, Ministry of Education Key Laboratory of Cell Proliferation and Differentiation, Beijing 100871, ChinaDepartment of Obstetrics and Gynecology, Beijing Advanced Innovation Center for Genomics, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, China; Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, China; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing 100191, ChinaDepartment of Obstetrics and Gynecology, Beijing Advanced Innovation Center for Genomics, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, China; Biomedical Institute for Pioneering Investigation via Convergence, Ministry of Education Key Laboratory of Cell Proliferation and Differentiation, Beijing 100871, China; Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, ChinaDepartment of Obstetrics and Gynecology, Beijing Advanced Innovation Center for Genomics, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, China; Biomedical Institute for Pioneering Investigation via Convergence, Ministry of Education Key Laboratory of Cell Proliferation and Differentiation, Beijing 100871, ChinaDepartment of Obstetrics and Gynecology, Beijing Advanced Innovation Center for Genomics, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, China; Biomedical Institute for Pioneering Investigation via Convergence, Ministry of Education Key Laboratory of Cell Proliferation and Differentiation, Beijing 100871, ChinaDepartment of Obstetrics and Gynecology, Beijing Advanced Innovation Center for Genomics, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, China; Biomedical Institute for Pioneering Investigation via Convergence, Ministry of Education Key Laboratory of Cell Proliferation and Differentiation, Beijing 100871, China; Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, ChinaDepartment of Obstetrics and Gynecology, Beijing Advanced Innovation Center for Genomics, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, China; Biomedical Institute for Pioneering Investigation via Convergence, Ministry of Education Key Laboratory of Cell Proliferation and Differentiation, Beijing 100871, ChinaDepartment of Obstetrics and Gynecology, Beijing Advanced Innovation Center for Genomics, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, China; Biomedical Institute for Pioneering Investigation via Convergence, Ministry of Education Key Laboratory of Cell Proliferation and Differentiation, Beijing 100871, China; Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, ChinaDepartment of Obstetrics and Gynecology, Beijing Advanced Innovation Center for Genomics, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, China; Biomedical Institute for Pioneering Investigation via Convergence, Ministry of Education Key Laboratory of Cell Proliferation and Differentiation, Beijing 100871, ChinaDepartment of Obstetrics and Gynecology, Beijing Advanced Innovation Center for Genomics, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, China; Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, China; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing 100191, China; Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China; Corresponding authorDepartment of Obstetrics and Gynecology, Beijing Advanced Innovation Center for Genomics, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, China; Biomedical Institute for Pioneering Investigation via Convergence, Ministry of Education Key Laboratory of Cell Proliferation and Differentiation, Beijing 100871, China; Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China; Corresponding authorSummary: The heart is the central organ of the circulatory system, and its proper development is vital for maintaining human life. Here, we used single-cell RNA sequencing to profile the gene expression landscapes of ∼4,000 cardiac cells from human embryos and identified four major types of cells: cardiomyocytes (CMs), cardiac fibroblasts, endothelial cells (ECs), and valvar interstitial cells (VICs). Atrial and ventricular CMs acquired distinct features early in heart development. Furthermore, both CMs and fibroblasts show stepwise changes in gene expression. As development proceeds, VICs may be involved in the remodeling phase, and ECs display location-specific characteristics. Finally, we compared gene expression profiles between humans and mice and identified a series of unique features of human heart development. Our study lays the groundwork for elucidating the mechanisms of in vivo human cardiac development and provides potential clues to understand cardiac regeneration. : Cui et al. reveal the transcriptional landscapes of human fetal heart development at single-cell resolution and identify critical biological features of different cell types, providing insights into the molecular mechanisms of human heart development. Keywords: human fetal heart development, single-cell RNA-seq, cardiomyocytes, cardiac fibroblasts, endothelial cells, valvar interstitial cells, extracellular matrix, cross-species comparison, single-cell transcriptome analysis, human embryonic developmenthttp://www.sciencedirect.com/science/article/pii/S2211124719301081 |