Brick Strex: a robust device built of LEGO bricks for mechanical manipulation of cells
Abstract Cellular forces, mechanics and other physical factors are important co-regulators of normal cell and tissue physiology. These cues are often misregulated in diseases such as cancer, where altered tissue mechanics contribute to the disease progression. Furthermore, intercellular tensile and...
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2021-09-01
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doaj-837b2cecffa1492ea17218b16ea206302021-09-19T11:31:10ZengNature Publishing GroupScientific Reports2045-23222021-09-0111111410.1038/s41598-021-97900-5Brick Strex: a robust device built of LEGO bricks for mechanical manipulation of cellsElina Mäntylä0Teemu O. Ihalainen1BioMediTech, Faculty of Medicine and Health Technology, Tampere UniversityBioMediTech, Faculty of Medicine and Health Technology, Tampere UniversityAbstract Cellular forces, mechanics and other physical factors are important co-regulators of normal cell and tissue physiology. These cues are often misregulated in diseases such as cancer, where altered tissue mechanics contribute to the disease progression. Furthermore, intercellular tensile and compressive force-related signaling is highlighted in collective cell behavior during development. However, the mechanistic understanding on the role of physical forces in regulation of cellular physiology, including gene expression and signaling, is still lacking. This is partly because studies on the molecular mechanisms of force transmission require easily controllable experimental designs. These approaches should enable both easy mechanical manipulation of cells and, importantly, readouts ranging from microscopy imaging to biochemical assays. To achieve a robust solution for mechanical manipulation of cells, we developed devices built of LEGO bricks allowing manual, motorized and/or cyclic cell stretching and compression studies. By using these devices, we show that $$\upbeta$$ β -catenin responds differentially to epithelial monolayer stretching and lateral compression, either localizing more to the cell nuclei or cell–cell junctions, respectively. In addition, we show that epithelial compression drives cytoplasmic retention and phosphorylation of transcription coregulator YAP1. We provide a complete part listing and video assembly instructions, allowing other researchers to build and use the devices in cellular mechanics-related studies.https://doi.org/10.1038/s41598-021-97900-5 |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Elina Mäntylä Teemu O. Ihalainen |
spellingShingle |
Elina Mäntylä Teemu O. Ihalainen Brick Strex: a robust device built of LEGO bricks for mechanical manipulation of cells Scientific Reports |
author_facet |
Elina Mäntylä Teemu O. Ihalainen |
author_sort |
Elina Mäntylä |
title |
Brick Strex: a robust device built of LEGO bricks for mechanical manipulation of cells |
title_short |
Brick Strex: a robust device built of LEGO bricks for mechanical manipulation of cells |
title_full |
Brick Strex: a robust device built of LEGO bricks for mechanical manipulation of cells |
title_fullStr |
Brick Strex: a robust device built of LEGO bricks for mechanical manipulation of cells |
title_full_unstemmed |
Brick Strex: a robust device built of LEGO bricks for mechanical manipulation of cells |
title_sort |
brick strex: a robust device built of lego bricks for mechanical manipulation of cells |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-09-01 |
description |
Abstract Cellular forces, mechanics and other physical factors are important co-regulators of normal cell and tissue physiology. These cues are often misregulated in diseases such as cancer, where altered tissue mechanics contribute to the disease progression. Furthermore, intercellular tensile and compressive force-related signaling is highlighted in collective cell behavior during development. However, the mechanistic understanding on the role of physical forces in regulation of cellular physiology, including gene expression and signaling, is still lacking. This is partly because studies on the molecular mechanisms of force transmission require easily controllable experimental designs. These approaches should enable both easy mechanical manipulation of cells and, importantly, readouts ranging from microscopy imaging to biochemical assays. To achieve a robust solution for mechanical manipulation of cells, we developed devices built of LEGO bricks allowing manual, motorized and/or cyclic cell stretching and compression studies. By using these devices, we show that $$\upbeta$$ β -catenin responds differentially to epithelial monolayer stretching and lateral compression, either localizing more to the cell nuclei or cell–cell junctions, respectively. In addition, we show that epithelial compression drives cytoplasmic retention and phosphorylation of transcription coregulator YAP1. We provide a complete part listing and video assembly instructions, allowing other researchers to build and use the devices in cellular mechanics-related studies. |
url |
https://doi.org/10.1038/s41598-021-97900-5 |
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