Biochemical and Molecular Characterization of Plant-Parasitic Nematodes
Nematologists need correct species identification to carry out research, teaching, extension and other activities. Therefore, nematode taxonomy must be pursued diligently at all levels. The identification of plant-parasitic nematodes is not always easy and that of some species is especially difficul...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Firenze University Press
2004-08-01
|
Series: | Phytopathologia Mediterranea |
Online Access: | https://oajournals.fupress.net/index.php/pm/article/view/5053 |
id |
doaj-838a77e922154dfe9fcb085eef7c2a79 |
---|---|
record_format |
Article |
spelling |
doaj-838a77e922154dfe9fcb085eef7c2a792020-11-25T03:04:44ZengFirenze University PressPhytopathologia Mediterranea0031-94651593-20952004-08-0143210.14601/Phytopathol_Mediterr-17411737Biochemical and Molecular Characterization of Plant-Parasitic NematodesI.M. de O. AbrantesM.C. Vieira dos SantosI. LuciP.M. da ConceiçaoM. José M. da CunhaM.S.N. de A. SantosNematologists need correct species identification to carry out research, teaching, extension and other activities. Therefore, nematode taxonomy must be pursued diligently at all levels. The identification of plant-parasitic nematodes is not always easy and that of some species is especially difficult. Most of the information that nematologists use when characterizing and identifying specimens is based on morphological and morphometrical characters. Although these characters are of primary importance, in the last three decades they have been supplemented by biochemical/ molecular characters. Biochemical approaches include the separation of proteins (general proteins and isozymes) by one-dimensional gel electrophoresis, isoelectric focusing, two-dimensional gel electrophoresis, and sodium dodecyl sulphate-capillary gel electrophoresis. Serology has also been found effective in the identification and quantification of nematodes, monoclonal antibodies being a more useful immunological tool than polyclonal antibodies. Identification based on the direct examination of DNA is potentially a more powerful method to characterize inter- and intra-specific variability. The development of techniques such as the polymerase chain reaction, restriction fragment length polymorphism, randomly amplified polymorphic DNA, and amplified fragment length polymorphism has increased the accuracy and speed of nematode characterization/identification. Progress continues to be made and more and more nematologists are using molecular techniques for diagnostic purposes and to assess genetic variation.https://oajournals.fupress.net/index.php/pm/article/view/5053 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
I.M. de O. Abrantes M.C. Vieira dos Santos I. Luci P.M. da Conceiçao M. José M. da Cunha M.S.N. de A. Santos |
spellingShingle |
I.M. de O. Abrantes M.C. Vieira dos Santos I. Luci P.M. da Conceiçao M. José M. da Cunha M.S.N. de A. Santos Biochemical and Molecular Characterization of Plant-Parasitic Nematodes Phytopathologia Mediterranea |
author_facet |
I.M. de O. Abrantes M.C. Vieira dos Santos I. Luci P.M. da Conceiçao M. José M. da Cunha M.S.N. de A. Santos |
author_sort |
I.M. de O. Abrantes |
title |
Biochemical and Molecular Characterization of Plant-Parasitic Nematodes |
title_short |
Biochemical and Molecular Characterization of Plant-Parasitic Nematodes |
title_full |
Biochemical and Molecular Characterization of Plant-Parasitic Nematodes |
title_fullStr |
Biochemical and Molecular Characterization of Plant-Parasitic Nematodes |
title_full_unstemmed |
Biochemical and Molecular Characterization of Plant-Parasitic Nematodes |
title_sort |
biochemical and molecular characterization of plant-parasitic nematodes |
publisher |
Firenze University Press |
series |
Phytopathologia Mediterranea |
issn |
0031-9465 1593-2095 |
publishDate |
2004-08-01 |
description |
Nematologists need correct species identification to carry out research, teaching, extension and other
activities. Therefore, nematode taxonomy must be pursued diligently at all levels. The identification of plant-parasitic
nematodes is not always easy and that of some species is especially difficult. Most of the information that nematologists
use when characterizing and identifying specimens is based on morphological and morphometrical characters. Although
these characters are of primary importance, in the last three decades they have been supplemented by biochemical/
molecular characters. Biochemical approaches include the separation of proteins (general proteins and
isozymes) by one-dimensional gel electrophoresis, isoelectric focusing, two-dimensional gel electrophoresis, and sodium
dodecyl sulphate-capillary gel electrophoresis. Serology has also been found effective in the identification and
quantification of nematodes, monoclonal antibodies being a more useful immunological tool than polyclonal antibodies.
Identification based on the direct examination of DNA is potentially a more powerful method to characterize
inter- and intra-specific variability. The development of techniques such as the polymerase chain reaction, restriction
fragment length polymorphism, randomly amplified polymorphic DNA, and amplified fragment length polymorphism
has increased the accuracy and speed of nematode characterization/identification. Progress continues to be
made and more and more nematologists are using molecular techniques for diagnostic purposes and to assess genetic
variation. |
url |
https://oajournals.fupress.net/index.php/pm/article/view/5053 |
work_keys_str_mv |
AT imdeoabrantes biochemicalandmolecularcharacterizationofplantparasiticnematodes AT mcvieiradossantos biochemicalandmolecularcharacterizationofplantparasiticnematodes AT iluci biochemicalandmolecularcharacterizationofplantparasiticnematodes AT pmdaconceicao biochemicalandmolecularcharacterizationofplantparasiticnematodes AT mjosemdacunha biochemicalandmolecularcharacterizationofplantparasiticnematodes AT msndeasantos biochemicalandmolecularcharacterizationofplantparasiticnematodes |
_version_ |
1724680075750670336 |