Bone Healing and Regeneration Potential in Rabbit Cortical Defects Using an Innovative Bioceramic Bone Graft Substitute
This study aimed to elucidate the local effect and micro-computed tomographic (μ-CT) assessment following bone implantation of an innovative bioceramic (α-calcium sulfate hemihydrate; α-CSH) on femur lateral condyle cortical bone of rabbit models. The innovative α-CSH bioceramic was synthesized thro...
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doaj-83af78ba37804e0bb6d003878fc7c4bc2020-11-25T03:00:26ZengMDPI AGApplied Sciences2076-34172020-09-01106239623910.3390/app10186239Bone Healing and Regeneration Potential in Rabbit Cortical Defects Using an Innovative Bioceramic Bone Graft SubstituteKeng-Liang Ou0Ping-Jen Hou1Bai-Hung Huang2Hsin-Hua Chou3Tzu-Sen Yang4Chiung-Fang Huang5Takaaki Ueno6Department of Dentistry, Taipei Medical University-Shuang Ho Hospital, New Taipei City 235, TaiwanGraduate Institute of Biomedical Materials and Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 110, TaiwanBiomedical Technology R & D Center, China Medical University Hospital, Taichung 404, TaiwanSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, TaiwanGraduate Institute of Biomedical Materials and Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 110, TaiwanSchool of Dental Technology, College of Oral Medicine, Taipei Medical University, Taipei 110, TaiwanDepartment of Dentistry and Oral Surgery, Osaka Medical College, Osaka 569-8686, JapanThis study aimed to elucidate the local effect and micro-computed tomographic (μ-CT) assessment following bone implantation of an innovative bioceramic (α-calcium sulfate hemihydrate; α-CSH) on femur lateral condyle cortical bone of rabbit models. The innovative α-CSH bioceramic was synthesized through a green processing technology (microwave irradiation treatment). The bilateral implantation model was performed among 24 New Zealand White rabbits which were divided into three groups based on the type of filling materials: α-CSH, control, and blank. Treatments were performed in defects with 6 mm diameter and 7 mm depth and observed after 2, 4, 8, and 12 weeks. Material reaction and bone formation after implantation were evaluated radiographically and histopathologically. The μ-CT analysis results showed that the degradation of α-CSH and control material was similar at 4 and 8 weeks. The bone volume in the defects indicated the α-CSH increased most in 8 weeks. In histopathological evaluation, the α-CSH group was repaired with lamellar bone and well-grown bone marrow infiltration similar to the control material. Moreover, the α-CSH revealed a faster degradation rate and better healing progress than the control material under the same conditions. Therefore, the α-CSH was confirmed to be useful in promoting osteoconduction and in controlling the resorption rate in bone defects. Further, the innovative α-CSH could be considered as a promising bone substitute for utilization in bone reconstructive therapy in dental and orthopedic fields.https://www.mdpi.com/2076-3417/10/18/6239calcium sulfate hemihydratebioceramicbone defectbone graft substitutebone regeneration |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Keng-Liang Ou Ping-Jen Hou Bai-Hung Huang Hsin-Hua Chou Tzu-Sen Yang Chiung-Fang Huang Takaaki Ueno |
spellingShingle |
Keng-Liang Ou Ping-Jen Hou Bai-Hung Huang Hsin-Hua Chou Tzu-Sen Yang Chiung-Fang Huang Takaaki Ueno Bone Healing and Regeneration Potential in Rabbit Cortical Defects Using an Innovative Bioceramic Bone Graft Substitute Applied Sciences calcium sulfate hemihydrate bioceramic bone defect bone graft substitute bone regeneration |
author_facet |
Keng-Liang Ou Ping-Jen Hou Bai-Hung Huang Hsin-Hua Chou Tzu-Sen Yang Chiung-Fang Huang Takaaki Ueno |
author_sort |
Keng-Liang Ou |
title |
Bone Healing and Regeneration Potential in Rabbit Cortical Defects Using an Innovative Bioceramic Bone Graft Substitute |
title_short |
Bone Healing and Regeneration Potential in Rabbit Cortical Defects Using an Innovative Bioceramic Bone Graft Substitute |
title_full |
Bone Healing and Regeneration Potential in Rabbit Cortical Defects Using an Innovative Bioceramic Bone Graft Substitute |
title_fullStr |
Bone Healing and Regeneration Potential in Rabbit Cortical Defects Using an Innovative Bioceramic Bone Graft Substitute |
title_full_unstemmed |
Bone Healing and Regeneration Potential in Rabbit Cortical Defects Using an Innovative Bioceramic Bone Graft Substitute |
title_sort |
bone healing and regeneration potential in rabbit cortical defects using an innovative bioceramic bone graft substitute |
publisher |
MDPI AG |
series |
Applied Sciences |
issn |
2076-3417 |
publishDate |
2020-09-01 |
description |
This study aimed to elucidate the local effect and micro-computed tomographic (μ-CT) assessment following bone implantation of an innovative bioceramic (α-calcium sulfate hemihydrate; α-CSH) on femur lateral condyle cortical bone of rabbit models. The innovative α-CSH bioceramic was synthesized through a green processing technology (microwave irradiation treatment). The bilateral implantation model was performed among 24 New Zealand White rabbits which were divided into three groups based on the type of filling materials: α-CSH, control, and blank. Treatments were performed in defects with 6 mm diameter and 7 mm depth and observed after 2, 4, 8, and 12 weeks. Material reaction and bone formation after implantation were evaluated radiographically and histopathologically. The μ-CT analysis results showed that the degradation of α-CSH and control material was similar at 4 and 8 weeks. The bone volume in the defects indicated the α-CSH increased most in 8 weeks. In histopathological evaluation, the α-CSH group was repaired with lamellar bone and well-grown bone marrow infiltration similar to the control material. Moreover, the α-CSH revealed a faster degradation rate and better healing progress than the control material under the same conditions. Therefore, the α-CSH was confirmed to be useful in promoting osteoconduction and in controlling the resorption rate in bone defects. Further, the innovative α-CSH could be considered as a promising bone substitute for utilization in bone reconstructive therapy in dental and orthopedic fields. |
topic |
calcium sulfate hemihydrate bioceramic bone defect bone graft substitute bone regeneration |
url |
https://www.mdpi.com/2076-3417/10/18/6239 |
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