Identification of Spanish isolates of <em>Rhizoctonia solani</em> from potato by anastomosis grouping, ITS-RFLP and RAMS-fingerprinting
Anastomosis grouping, restriction fragment length polymorphism (RFLP) of the ITS regions including the 5.85 rDNA, and random amplified microsatellites (RAMS) were used to characterize isolates of Rhizoctonia solani collected from Spain and Finland. There was a high similarity between the results obt...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Firenze University Press
2003-08-01
|
| Series: | Phytopathologia Mediterranea |
| Online Access: | https://oajournals.fupress.net/index.php/pm/article/view/5010 |
| Summary: | Anastomosis grouping, restriction fragment length polymorphism (RFLP) of the ITS regions including
the 5.85 rDNA, and random amplified microsatellites (RAMS) were used to characterize isolates of Rhizoctonia
solani collected from Spain and Finland. There was a high similarity between the results obtained with the three
techniques. RAMS markers revealed more genetic variation among isolates of R. solani than RFLP. The anastomosis
group (AG)–3 isolates were clearly separated from isolates belonging to other AGs by RAMS, RFLPs and anastomosis
grouping. Almost all the isolates sampled from potato belonged to AG–3. No differences were observed between
Spanish and Finnish AG–3 isolates. |
|---|---|
| ISSN: | 0031-9465 1593-2095 |