Identification of Spanish isolates of <em>Rhizoctonia solani</em> from potato by anastomosis grouping, ITS-RFLP and RAMS-fingerprinting

Anastomosis grouping, restriction fragment length polymorphism (RFLP) of the ITS regions including the 5.85 rDNA, and random amplified microsatellites (RAMS) were used to characterize isolates of Rhizoctonia solani collected from Spain and Finland. There was a high similarity between the results obt...

Full description

Bibliographic Details
Main Authors: A.M. Elbakali, A. Lilja, J. Hantula, M.P: Martín
Format: Article
Language:English
Published: Firenze University Press 2003-08-01
Series:Phytopathologia Mediterranea
Online Access:https://oajournals.fupress.net/index.php/pm/article/view/5010
id doaj-83b13e11e1c14a04bd59127c5ed891be
record_format Article
spelling doaj-83b13e11e1c14a04bd59127c5ed891be2020-11-25T03:18:50ZengFirenze University PressPhytopathologia Mediterranea0031-94651593-20952003-08-0142210.14601/Phytopathol_Mediterr-17021698Identification of Spanish isolates of <em>Rhizoctonia solani</em> from potato by anastomosis grouping, ITS-RFLP and RAMS-fingerprintingA.M. ElbakaliA. LiljaJ. HantulaM.P: MartínAnastomosis grouping, restriction fragment length polymorphism (RFLP) of the ITS regions including the 5.85 rDNA, and random amplified microsatellites (RAMS) were used to characterize isolates of Rhizoctonia solani collected from Spain and Finland. There was a high similarity between the results obtained with the three techniques. RAMS markers revealed more genetic variation among isolates of R. solani than RFLP. The anastomosis group (AG)–3 isolates were clearly separated from isolates belonging to other AGs by RAMS, RFLPs and anastomosis grouping. Almost all the isolates sampled from potato belonged to AG–3. No differences were observed between Spanish and Finnish AG–3 isolates.https://oajournals.fupress.net/index.php/pm/article/view/5010
collection DOAJ
language English
format Article
sources DOAJ
author A.M. Elbakali
A. Lilja
J. Hantula
M.P: Martín
spellingShingle A.M. Elbakali
A. Lilja
J. Hantula
M.P: Martín
Identification of Spanish isolates of <em>Rhizoctonia solani</em> from potato by anastomosis grouping, ITS-RFLP and RAMS-fingerprinting
Phytopathologia Mediterranea
author_facet A.M. Elbakali
A. Lilja
J. Hantula
M.P: Martín
author_sort A.M. Elbakali
title Identification of Spanish isolates of <em>Rhizoctonia solani</em> from potato by anastomosis grouping, ITS-RFLP and RAMS-fingerprinting
title_short Identification of Spanish isolates of <em>Rhizoctonia solani</em> from potato by anastomosis grouping, ITS-RFLP and RAMS-fingerprinting
title_full Identification of Spanish isolates of <em>Rhizoctonia solani</em> from potato by anastomosis grouping, ITS-RFLP and RAMS-fingerprinting
title_fullStr Identification of Spanish isolates of <em>Rhizoctonia solani</em> from potato by anastomosis grouping, ITS-RFLP and RAMS-fingerprinting
title_full_unstemmed Identification of Spanish isolates of <em>Rhizoctonia solani</em> from potato by anastomosis grouping, ITS-RFLP and RAMS-fingerprinting
title_sort identification of spanish isolates of <em>rhizoctonia solani</em> from potato by anastomosis grouping, its-rflp and rams-fingerprinting
publisher Firenze University Press
series Phytopathologia Mediterranea
issn 0031-9465
1593-2095
publishDate 2003-08-01
description Anastomosis grouping, restriction fragment length polymorphism (RFLP) of the ITS regions including the 5.85 rDNA, and random amplified microsatellites (RAMS) were used to characterize isolates of Rhizoctonia solani collected from Spain and Finland. There was a high similarity between the results obtained with the three techniques. RAMS markers revealed more genetic variation among isolates of R. solani than RFLP. The anastomosis group (AG)–3 isolates were clearly separated from isolates belonging to other AGs by RAMS, RFLPs and anastomosis grouping. Almost all the isolates sampled from potato belonged to AG–3. No differences were observed between Spanish and Finnish AG–3 isolates.
url https://oajournals.fupress.net/index.php/pm/article/view/5010
work_keys_str_mv AT amelbakali identificationofspanishisolatesofemrhizoctoniasolaniemfrompotatobyanastomosisgroupingitsrflpandramsfingerprinting
AT alilja identificationofspanishisolatesofemrhizoctoniasolaniemfrompotatobyanastomosisgroupingitsrflpandramsfingerprinting
AT jhantula identificationofspanishisolatesofemrhizoctoniasolaniemfrompotatobyanastomosisgroupingitsrflpandramsfingerprinting
AT mpmartin identificationofspanishisolatesofemrhizoctoniasolaniemfrompotatobyanastomosisgroupingitsrflpandramsfingerprinting
_version_ 1724625600933527552