Biodegradable Polymers Induce CD54 on THP-1 Cells in Skin Sensitization Test
Currently, nonanimal methods of skin sensitization testing for various chemicals, biodegradable polymers, and biomaterials are being developed in the hope of eliminating the use of animals. The human cell line activation test (h-CLAT) is a skin sensitization assessment that mimics the functions of d...
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doaj-83d20e2d5c8f464fb0b13993a01e22f92020-11-24T20:54:31ZengHindawi LimitedInternational Journal of Biomaterials1687-87871687-87952011-01-01201110.1155/2011/424571424571Biodegradable Polymers Induce CD54 on THP-1 Cells in Skin Sensitization TestYeon Suk Jung0Reiko Kato1Toshie Tsuchiya2Division of Medical Devices, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, JapanDivision of Medical Devices, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, JapanDivision of Medical Devices, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, JapanCurrently, nonanimal methods of skin sensitization testing for various chemicals, biodegradable polymers, and biomaterials are being developed in the hope of eliminating the use of animals. The human cell line activation test (h-CLAT) is a skin sensitization assessment that mimics the functions of dendritic cells (DCs). DCs are specialized antigen-presenting cells, and they interact with T cells and B cells to initiate immune responses. Phenotypic changes in DCs, such as the production of CD86 and CD54 and internalization of MHC class II molecules, have become focal points of the skin sensitization test. In this study, we used h-CLAT to assess the effects of biodegradable polymers. The results showed that several biodegradable polymers increased the expression of CD54, and the relative skin sensitizing abilities of biodegradable polymers were PLLG (75 : 25) < PLLC (40 : 60) < PLGA (50 : 50) < PCG (50 : 50). These results may contribute to the creation of new guidelines for the use of biodegradable polymers in scaffolds or allergenic hazards.http://dx.doi.org/10.1155/2011/424571 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yeon Suk Jung Reiko Kato Toshie Tsuchiya |
spellingShingle |
Yeon Suk Jung Reiko Kato Toshie Tsuchiya Biodegradable Polymers Induce CD54 on THP-1 Cells in Skin Sensitization Test International Journal of Biomaterials |
author_facet |
Yeon Suk Jung Reiko Kato Toshie Tsuchiya |
author_sort |
Yeon Suk Jung |
title |
Biodegradable Polymers Induce CD54 on THP-1 Cells in Skin Sensitization Test |
title_short |
Biodegradable Polymers Induce CD54 on THP-1 Cells in Skin Sensitization Test |
title_full |
Biodegradable Polymers Induce CD54 on THP-1 Cells in Skin Sensitization Test |
title_fullStr |
Biodegradable Polymers Induce CD54 on THP-1 Cells in Skin Sensitization Test |
title_full_unstemmed |
Biodegradable Polymers Induce CD54 on THP-1 Cells in Skin Sensitization Test |
title_sort |
biodegradable polymers induce cd54 on thp-1 cells in skin sensitization test |
publisher |
Hindawi Limited |
series |
International Journal of Biomaterials |
issn |
1687-8787 1687-8795 |
publishDate |
2011-01-01 |
description |
Currently, nonanimal methods of skin sensitization testing for various chemicals, biodegradable polymers, and biomaterials are being developed in the hope of eliminating the use of animals. The human cell line activation test (h-CLAT) is a skin sensitization assessment that mimics the functions of dendritic cells (DCs). DCs are specialized antigen-presenting cells, and they interact with T cells and B cells to initiate immune responses. Phenotypic changes in DCs, such as the production of CD86 and CD54 and internalization of MHC class II molecules, have become focal points of the skin sensitization test. In this study, we used h-CLAT to assess the effects of biodegradable polymers. The results showed that several biodegradable polymers increased the expression of CD54, and the relative skin sensitizing abilities of biodegradable polymers were PLLG (75 : 25) < PLLC (40 : 60) < PLGA (50 : 50) < PCG (50 : 50). These results may contribute to the creation of new guidelines for the use of biodegradable polymers in scaffolds or allergenic hazards. |
url |
http://dx.doi.org/10.1155/2011/424571 |
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