Inflammation Biomarkers of Advanced Disease in Nongingival Tissues of Chronic Periodontitis Patients
Chronic periodontitis is a multifactorial inflammatory disease that affects supporting structures of the teeth. Although the gingival response is largely described, little is known about the immune changes in the alveolar bone and neighboring tissues that could indicate periodontal disease (PD) acti...
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doaj-83f3051c770141d0ad937d3daf2efbbd2020-11-25T00:52:44ZengHindawi LimitedMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/983782983782Inflammation Biomarkers of Advanced Disease in Nongingival Tissues of Chronic Periodontitis PatientsThiago Alvares da Costa0Marcelo José Barbosa Silva1Polyanna Miranda Alves2Javier Emílio Lazo Chica3Emilio Zorzo Barcelos4Max Antonio Alves Giani5Gustavo Pompermaier Garlet6João Santana da Silva7Virmondes Rodrigues Júnior8Denise Bertulucci Rocha Rodrigues9Cristina Ribeiro de Barros Cardoso10Instituto de Ciências Biológicas e Naturais, Universidade Federal do Triângulo Mineiro, Uberaba, MG, BrazilInstituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, MG, BrazilInstituto de Ciências Biológicas e Naturais, Universidade Federal do Triângulo Mineiro, Uberaba, MG, BrazilInstituto de Ciências Biológicas e Naturais, Universidade Federal do Triângulo Mineiro, Uberaba, MG, BrazilInstituto de Ciências Tecnológicas e Exatas, Universidade Federal do Triângulo Mineiro, Uberaba, MG, BrazilDepartamento de Odontologia Clínica, Universidade de Uberaba, Uberaba, MG, BrazilDepartamento de Biologia Oral, Faculdade de Odontologia de Bauru, Universidade de São Paulo, Bauru, SP, BrazilDepartamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, BrazilInstituto de Ciências Biológicas e Naturais, Universidade Federal do Triângulo Mineiro, Uberaba, MG, BrazilDepartamento de Odontologia Clínica, Universidade de Uberaba, Uberaba, MG, BrazilInstituto de Ciências Biológicas e Naturais, Universidade Federal do Triângulo Mineiro, Uberaba, MG, BrazilChronic periodontitis is a multifactorial inflammatory disease that affects supporting structures of the teeth. Although the gingival response is largely described, little is known about the immune changes in the alveolar bone and neighboring tissues that could indicate periodontal disease (PD) activity. Then, in this study we identified the ongoing inflammatory changes and novel biomarkers for periodontitis in the tissues directly affected by the destructive disease in PD patients. Samples were collected by osteotomy in 17 control subjects during extraction of third molars and 18 patients with advanced PD, in which alveoloplasty was necessary after extraction of teeth with previous extensive periodontal damage. Patients presented mononuclear cells infiltration in the connective tissue next to the bone and higher fibrosis area, along with increased accumulation of IL-17+ and TRAP+ cells. The levels of TNF-α and MMP-2 mRNA were also elevated compared to controls and a positive and significant correlation was observed between TNF-α and MMP-2 mRNA expression, considering all samples evaluated. In conclusion, nongingival tissues neighboring large periodontal pockets present inflammatory markers that could predict ongoing bone resorption and disease spreading. Therefore, we suggested that the detailed evaluation of these regions could be of great importance to the assessment of disease progression.http://dx.doi.org/10.1155/2015/983782 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Thiago Alvares da Costa Marcelo José Barbosa Silva Polyanna Miranda Alves Javier Emílio Lazo Chica Emilio Zorzo Barcelos Max Antonio Alves Giani Gustavo Pompermaier Garlet João Santana da Silva Virmondes Rodrigues Júnior Denise Bertulucci Rocha Rodrigues Cristina Ribeiro de Barros Cardoso |
spellingShingle |
Thiago Alvares da Costa Marcelo José Barbosa Silva Polyanna Miranda Alves Javier Emílio Lazo Chica Emilio Zorzo Barcelos Max Antonio Alves Giani Gustavo Pompermaier Garlet João Santana da Silva Virmondes Rodrigues Júnior Denise Bertulucci Rocha Rodrigues Cristina Ribeiro de Barros Cardoso Inflammation Biomarkers of Advanced Disease in Nongingival Tissues of Chronic Periodontitis Patients Mediators of Inflammation |
author_facet |
Thiago Alvares da Costa Marcelo José Barbosa Silva Polyanna Miranda Alves Javier Emílio Lazo Chica Emilio Zorzo Barcelos Max Antonio Alves Giani Gustavo Pompermaier Garlet João Santana da Silva Virmondes Rodrigues Júnior Denise Bertulucci Rocha Rodrigues Cristina Ribeiro de Barros Cardoso |
author_sort |
Thiago Alvares da Costa |
title |
Inflammation Biomarkers of Advanced Disease in Nongingival Tissues of Chronic Periodontitis Patients |
title_short |
Inflammation Biomarkers of Advanced Disease in Nongingival Tissues of Chronic Periodontitis Patients |
title_full |
Inflammation Biomarkers of Advanced Disease in Nongingival Tissues of Chronic Periodontitis Patients |
title_fullStr |
Inflammation Biomarkers of Advanced Disease in Nongingival Tissues of Chronic Periodontitis Patients |
title_full_unstemmed |
Inflammation Biomarkers of Advanced Disease in Nongingival Tissues of Chronic Periodontitis Patients |
title_sort |
inflammation biomarkers of advanced disease in nongingival tissues of chronic periodontitis patients |
publisher |
Hindawi Limited |
series |
Mediators of Inflammation |
issn |
0962-9351 1466-1861 |
publishDate |
2015-01-01 |
description |
Chronic periodontitis is a multifactorial inflammatory disease that affects supporting structures of the teeth. Although the gingival response is largely described, little is known about the immune changes in the alveolar bone and neighboring tissues that could indicate periodontal disease (PD) activity. Then, in this study we identified the ongoing inflammatory changes and novel biomarkers for periodontitis in the tissues directly affected by the destructive disease in PD patients. Samples were collected by osteotomy in 17 control subjects during extraction of third molars and 18 patients with advanced PD, in which alveoloplasty was necessary after extraction of teeth with previous extensive periodontal damage. Patients presented mononuclear cells infiltration in the connective tissue next to the bone and higher fibrosis area, along with increased accumulation of IL-17+ and TRAP+ cells. The levels of TNF-α and MMP-2 mRNA were also elevated compared to controls and a positive and significant correlation was observed between TNF-α and MMP-2 mRNA expression, considering all samples evaluated. In conclusion, nongingival tissues neighboring large periodontal pockets present inflammatory markers that could predict ongoing bone resorption and disease spreading. Therefore, we suggested that the detailed evaluation of these regions could be of great importance to the assessment of disease progression. |
url |
http://dx.doi.org/10.1155/2015/983782 |
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