Grafting of Gallic Acid onto a Bioactive Ti6Al4V Alloy: A Physico-Chemical Characterization
Despite increasing interest in the use of natural biomolecules for different applications, few attempts of coupling them to inorganic biomaterials are reported in literature. Functionalization of metal implants with natural biomolecules could allow a local action, overcoming the issue of low bioavai...
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doaj-8401fae8f7d349feb2e978354ec5271a2020-11-25T02:03:38ZengMDPI AGCoatings2079-64122019-05-019530210.3390/coatings9050302coatings9050302Grafting of Gallic Acid onto a Bioactive Ti6Al4V Alloy: A Physico-Chemical CharacterizationMartina Cazzola0Sara Ferraris1Enrico Prenesti2Valentina Casalegno3Silvia Spriano4Department of Applied Science and Technology, Politecnico di Torino, C.so Duca degli Abruzzi 24, 10129 Torino, ItalyDepartment of Applied Science and Technology, Politecnico di Torino, C.so Duca degli Abruzzi 24, 10129 Torino, ItalyDepartment of Chemistry, Università degli Studi di Torino, Via Pietro Giuria 5, 10125 Torino, ItalyDepartment of Applied Science and Technology, Politecnico di Torino, C.so Duca degli Abruzzi 24, 10129 Torino, ItalyDepartment of Applied Science and Technology, Politecnico di Torino, C.so Duca degli Abruzzi 24, 10129 Torino, ItalyDespite increasing interest in the use of natural biomolecules for different applications, few attempts of coupling them to inorganic biomaterials are reported in literature. Functionalization of metal implants with natural biomolecules could allow a local action, overcoming the issue of low bioavailability through systemic administration. In the present work, gallic acid was grafted to a pre-treated Ti6Al4V in order to improve its biological response in bone contact applications. The grafting procedure was optimized by choosing the concentration of gallic acid (1 mg/mL) and the solvent of the solution, which was used as a source for functionalization, in order to maximize the amount of the grafted molecule on the titanium substrate. The functionalized surfaces were characterized. The results showed that functionalization with Simulated Body Fluid (SBF) as solvent medium was the most effective in terms of the amount and activity of the grafted biomolecule. A key role of calcium ions in the grafting mechanism is suggested, involving the formation of coordination compounds formed by way of gallic acid carboxylate and Ti−O<sup>−</sup> as oxygenated donor groups. Bioactive behavior and surface charge of the pre-treated Ti6Al4V surface were conserved after functionalization. The functionalized surface exposed a greater amount of OH groups and showed higher wettability.https://www.mdpi.com/2079-6412/9/5/302titaniumgallic acidpolyphenolssurface functionalizationmetal implants |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Martina Cazzola Sara Ferraris Enrico Prenesti Valentina Casalegno Silvia Spriano |
spellingShingle |
Martina Cazzola Sara Ferraris Enrico Prenesti Valentina Casalegno Silvia Spriano Grafting of Gallic Acid onto a Bioactive Ti6Al4V Alloy: A Physico-Chemical Characterization Coatings titanium gallic acid polyphenols surface functionalization metal implants |
author_facet |
Martina Cazzola Sara Ferraris Enrico Prenesti Valentina Casalegno Silvia Spriano |
author_sort |
Martina Cazzola |
title |
Grafting of Gallic Acid onto a Bioactive Ti6Al4V Alloy: A Physico-Chemical Characterization |
title_short |
Grafting of Gallic Acid onto a Bioactive Ti6Al4V Alloy: A Physico-Chemical Characterization |
title_full |
Grafting of Gallic Acid onto a Bioactive Ti6Al4V Alloy: A Physico-Chemical Characterization |
title_fullStr |
Grafting of Gallic Acid onto a Bioactive Ti6Al4V Alloy: A Physico-Chemical Characterization |
title_full_unstemmed |
Grafting of Gallic Acid onto a Bioactive Ti6Al4V Alloy: A Physico-Chemical Characterization |
title_sort |
grafting of gallic acid onto a bioactive ti6al4v alloy: a physico-chemical characterization |
publisher |
MDPI AG |
series |
Coatings |
issn |
2079-6412 |
publishDate |
2019-05-01 |
description |
Despite increasing interest in the use of natural biomolecules for different applications, few attempts of coupling them to inorganic biomaterials are reported in literature. Functionalization of metal implants with natural biomolecules could allow a local action, overcoming the issue of low bioavailability through systemic administration. In the present work, gallic acid was grafted to a pre-treated Ti6Al4V in order to improve its biological response in bone contact applications. The grafting procedure was optimized by choosing the concentration of gallic acid (1 mg/mL) and the solvent of the solution, which was used as a source for functionalization, in order to maximize the amount of the grafted molecule on the titanium substrate. The functionalized surfaces were characterized. The results showed that functionalization with Simulated Body Fluid (SBF) as solvent medium was the most effective in terms of the amount and activity of the grafted biomolecule. A key role of calcium ions in the grafting mechanism is suggested, involving the formation of coordination compounds formed by way of gallic acid carboxylate and Ti−O<sup>−</sup> as oxygenated donor groups. Bioactive behavior and surface charge of the pre-treated Ti6Al4V surface were conserved after functionalization. The functionalized surface exposed a greater amount of OH groups and showed higher wettability. |
topic |
titanium gallic acid polyphenols surface functionalization metal implants |
url |
https://www.mdpi.com/2079-6412/9/5/302 |
work_keys_str_mv |
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