An Exploratory Study on the Development of an Animal Model of Acute Pancreatitis Following Nicotine Exposure
Cigarette smoking is known to be a major risk factor for pancreatic cancer and pancreatitis is believed to be a predisposed condition for pancreatic cancer. As of this date, there is no established experimental animal model to conduct detailed studies on these two deadly diseases. Our aim is to esta...
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doaj-8414df31be7f42df8a75f67be99030862020-11-24T21:51:53ZengEuropean PublishingTobacco Induced Diseases1617-96252003-09-011September10.1186/1617-9625-1-3-21365412An Exploratory Study on the Development of an Animal Model of Acute Pancreatitis Following Nicotine ExposureP Chowdhury0Department of Physiology & Biophysics, University of Arkansas for Medical SciencesCigarette smoking is known to be a major risk factor for pancreatic cancer and pancreatitis is believed to be a predisposed condition for pancreatic cancer. As of this date, there is no established experimental animal model to conduct detailed studies on these two deadly diseases. Our aim is to establish a rodent model by which we can systematically study the pathogenesis of pancreatitis and pancreatic cancer. Methods Adult Male Sprague Dawley rats were exposed to graded doses of nicotine by various routes for periods of three to 16 weeks. Blood samples were measured for hormonal and metabolic parameters. The pancreas was evaluated for histopathological changes and its function was assessed in isolated pancreatic acini upon stimulation with cholecystokinin (CCK) or carbachol (Cch). The pancreatic tissue was evaluated further for oncogene expression. Results Body weight, food and fluid intakes, plasma glucose and insulin levels were significantly reduced in animals with nicotine exposure when compared to control. However, CCK and gastrin levels in the blood were significantly elevated. Pancreatic function was decreased significantly with no alteration in CCK receptor binding. Pancreatic histology revealed vacuolation, swelling, cellular pyknosis and karyorrhexis. Mutant oncogene, H-ras, was overexpressed in nicotinetreated pancreatic tissue Summary and conclusion The results suggest that alterations in metabolic, hormonal and pathologic parameters following nicotine-treatment appear consistent with diagnostic criteria of human pancreatitis. It is proposed that rats could be considered as a potential animal model to study the pathogenesis of pancreatitis.http://www.journalssystem.com/tid/An-Exploratory-Study-on-the-Development-of-an-Animal-Model-of-Acute-Pancreatitis-Following-Nicotine-Exposure,65412,0,2.htmlsmokingpancreaspancreatitispancreatic canceranimal model |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
P Chowdhury |
spellingShingle |
P Chowdhury An Exploratory Study on the Development of an Animal Model of Acute Pancreatitis Following Nicotine Exposure Tobacco Induced Diseases smoking pancreas pancreatitis pancreatic cancer animal model |
author_facet |
P Chowdhury |
author_sort |
P Chowdhury |
title |
An Exploratory Study on the Development of an Animal Model of Acute Pancreatitis Following Nicotine Exposure |
title_short |
An Exploratory Study on the Development of an Animal Model of Acute Pancreatitis Following Nicotine Exposure |
title_full |
An Exploratory Study on the Development of an Animal Model of Acute Pancreatitis Following Nicotine Exposure |
title_fullStr |
An Exploratory Study on the Development of an Animal Model of Acute Pancreatitis Following Nicotine Exposure |
title_full_unstemmed |
An Exploratory Study on the Development of an Animal Model of Acute Pancreatitis Following Nicotine Exposure |
title_sort |
exploratory study on the development of an animal model of acute pancreatitis following nicotine exposure |
publisher |
European Publishing |
series |
Tobacco Induced Diseases |
issn |
1617-9625 |
publishDate |
2003-09-01 |
description |
Cigarette smoking is known to be a major risk factor for pancreatic cancer
and pancreatitis is believed to be a predisposed condition for pancreatic cancer. As of this
date, there is no established experimental animal model to conduct detailed studies on these
two deadly diseases. Our aim is to establish a rodent model by which we can systematically
study the pathogenesis of pancreatitis and pancreatic cancer.
Methods
Adult Male Sprague
Dawley rats were exposed to graded doses of nicotine by various routes for periods of three
to 16 weeks. Blood samples were measured for hormonal and metabolic parameters. The
pancreas was evaluated for histopathological changes and its function was assessed in isolated
pancreatic acini upon stimulation with cholecystokinin (CCK) or carbachol (Cch). The
pancreatic tissue was evaluated further for oncogene expression.
Results
Body weight, food
and fluid intakes, plasma glucose and insulin levels were significantly reduced in animals
with nicotine exposure when compared to control. However, CCK and gastrin levels in the
blood were significantly elevated. Pancreatic function was decreased significantly with no
alteration in CCK receptor binding. Pancreatic histology revealed vacuolation, swelling,
cellular pyknosis and karyorrhexis. Mutant oncogene, H-ras, was overexpressed in nicotinetreated
pancreatic tissue
Summary and conclusion
The results suggest that alterations in
metabolic, hormonal and pathologic parameters following nicotine-treatment appear consistent
with diagnostic criteria of human pancreatitis. It is proposed that rats could be considered
as a potential animal model to study the pathogenesis of pancreatitis. |
topic |
smoking pancreas pancreatitis pancreatic cancer animal model |
url |
http://www.journalssystem.com/tid/An-Exploratory-Study-on-the-Development-of-an-Animal-Model-of-Acute-Pancreatitis-Following-Nicotine-Exposure,65412,0,2.html |
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