Panel of informative SNP markers for two genetic lines of European bison: Lowland and Lowland–Caucasian
As the result of a population bottleneck, the present population of European bison shows a high level of inbreeding, and a significant loss of genetic variability. In studies on such specific species there is a need to apply methods that obtain as much information about the genome as possible in a s...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Museu de Ciències Naturals de Barcelona
2017-01-01
|
Series: | Animal Biodiversity and Conservation |
Subjects: | |
Online Access: | http://abc.museucienciesjournals.cat/files/ABC_40-1_pp_17-25-color.pdf |
id |
doaj-842b12ae326a41fbb702e8ee63f4921b |
---|---|
record_format |
Article |
spelling |
doaj-842b12ae326a41fbb702e8ee63f4921b2020-11-25T00:57:27ZengMuseu de Ciències Naturals de BarcelonaAnimal Biodiversity and Conservation1578-665X2017-01-014011725Panel of informative SNP markers for two genetic lines of European bison: Lowland and Lowland–CaucasianWojciechowska, MNowak, Z.Gurgul, A. Olech, W.Drobik, W.Szmatoła, T. As the result of a population bottleneck, the present population of European bison shows a high level of inbreeding, and a significant loss of genetic variability. In studies on such specific species there is a need to apply methods that obtain as much information about the genome as possible in a short time. The aim of the study was to define a panel of SNP (single nucleotide polymorphism) markers that could serve in genetic diversity analysis of European bison from two lines: Lowland (LB) and Lowland–Caucasian (LC). The study used 57 individuals from the LB line and 72 from the LC line. To identify well–performing SNPs in European bison, we used two microarrays with different markers densities: BovineSNP50 v2 BeadChip and BovineHD BeadChip. As a result of the adopted criteria, 1,421 and 22,122 markers, respectively, were selected. On the basis of statistical analysis (allele frequencies, Fisher’s exact test, and the Z–test), a panel of 1,536 informative SNP markers was ultimately selected for further study; 26 of these with private alleles for the LB line and 611 with private alleles for the LC line. The data obtained in this study could further enrich and support breeding programs in the context of relatedness between particular specimens and herds from captive breeding centres.http://abc.museucienciesjournals.cat/files/ABC_40-1_pp_17-25-color.pdfBison bonasusEuropean bisonGenetic markerMicroarraySingle Nucleotide Polymorphism |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wojciechowska, M Nowak, Z. Gurgul, A. Olech, W. Drobik, W. Szmatoła, T. |
spellingShingle |
Wojciechowska, M Nowak, Z. Gurgul, A. Olech, W. Drobik, W. Szmatoła, T. Panel of informative SNP markers for two genetic lines of European bison: Lowland and Lowland–Caucasian Animal Biodiversity and Conservation Bison bonasus European bison Genetic marker Microarray Single Nucleotide Polymorphism |
author_facet |
Wojciechowska, M Nowak, Z. Gurgul, A. Olech, W. Drobik, W. Szmatoła, T. |
author_sort |
Wojciechowska, M |
title |
Panel of informative SNP markers for two genetic lines of European bison: Lowland and Lowland–Caucasian |
title_short |
Panel of informative SNP markers for two genetic lines of European bison: Lowland and Lowland–Caucasian |
title_full |
Panel of informative SNP markers for two genetic lines of European bison: Lowland and Lowland–Caucasian |
title_fullStr |
Panel of informative SNP markers for two genetic lines of European bison: Lowland and Lowland–Caucasian |
title_full_unstemmed |
Panel of informative SNP markers for two genetic lines of European bison: Lowland and Lowland–Caucasian |
title_sort |
panel of informative snp markers for two genetic lines of european bison: lowland and lowland–caucasian |
publisher |
Museu de Ciències Naturals de Barcelona |
series |
Animal Biodiversity and Conservation |
issn |
1578-665X |
publishDate |
2017-01-01 |
description |
As the result of a population bottleneck, the present population of European bison shows a high level of inbreeding, and a significant loss of genetic variability. In studies on such specific species there is a need to apply methods that obtain as much information about the genome as possible in a short time. The aim of the study was to define a panel of SNP (single nucleotide polymorphism) markers that could serve in genetic diversity analysis of European bison from two lines: Lowland (LB) and Lowland–Caucasian (LC). The study used 57 individuals from the LB line and 72 from the LC line. To identify well–performing SNPs in European bison, we used two microarrays with different markers densities: BovineSNP50 v2 BeadChip and BovineHD BeadChip. As a result of the adopted criteria, 1,421 and 22,122 markers, respectively, were selected. On the basis of statistical analysis (allele frequencies, Fisher’s exact test, and the Z–test), a panel of 1,536 informative SNP markers was ultimately selected for further study; 26 of these with private alleles for the LB line and 611 with private alleles for the LC line. The data obtained in this study could further enrich and support breeding programs in the context of relatedness between particular specimens and herds from captive breeding centres. |
topic |
Bison bonasus European bison Genetic marker Microarray Single Nucleotide Polymorphism |
url |
http://abc.museucienciesjournals.cat/files/ABC_40-1_pp_17-25-color.pdf |
work_keys_str_mv |
AT wojciechowskam panelofinformativesnpmarkersfortwogeneticlinesofeuropeanbisonlowlandandlowlandcaucasian AT nowakz panelofinformativesnpmarkersfortwogeneticlinesofeuropeanbisonlowlandandlowlandcaucasian AT gurgula panelofinformativesnpmarkersfortwogeneticlinesofeuropeanbisonlowlandandlowlandcaucasian AT olechw panelofinformativesnpmarkersfortwogeneticlinesofeuropeanbisonlowlandandlowlandcaucasian AT drobikw panelofinformativesnpmarkersfortwogeneticlinesofeuropeanbisonlowlandandlowlandcaucasian AT szmatołat panelofinformativesnpmarkersfortwogeneticlinesofeuropeanbisonlowlandandlowlandcaucasian |
_version_ |
1725224127352012800 |