Specific titin and myomesin domains stimulate myoblast proliferation

• Main Authors: Irina V. Kravchenko, Vladimir A. Furalyov, Vladimir O. Popov
• Format: Article
• Language: English
• Published: Elsevier 2017-03-01
• Series: Biochemistry and Biophysics Reports
• Subjects: Myoblast proliferation; Titin; Myomesin; Fn type III domain; Ig-like domain; IGF-1
• Online Access: http://www.sciencedirect.com/science/article/pii/S2405580816301947

Myofibrillar proteins titin and myomesin stimulated myoblast proliferation as determined by MTT-test and labelled thymidine incorporation in the DNA. Specific Fn type III and Ig-like domains of these proteins were able to exert mitogenic effects as well. Proliferative effect of Fn type III domains was highly sensitive to inhibition of Ca2+/calmodulin dependent protein kinase, whereas the effect of Ig-like domains showed greater sensitivity to the inhibition of adenylyl cyclase – cAMP – PKA pathway. IGF-1 autocrine signalling inhibition partially suppressed mitogenic effects revealed by both domain types.