Anti-nociceptive and anti-inflammatory effects of the methanolic extract of Opuntia humifusa stem

• Main Authors: Bhesh Raj Sharma, Chul Min Park, Jong Choi, Dong Young Rhyu
• Format: Article
• Language: English
• Published: Mashhad University of Medical Sciences 2017-06-01
• Series: Avicenna Journal of Phytomedicine
• Subjects: Opuntia humifusa; Anti-nociceptive; Anti-inflammatory; RAW 264.7 cells
• Online Access: http://ajp.mums.ac.ir/article_8245_a043ea0a8db52919588c1132374034e2.pdf
• ISSN: 2228-7930; 2228-7949

Objective: Opuntia humifusa (O. humifusa) Raf. has been used for the prevention and treatment of rheumatoid arthritis, inflammation, and cancer. Our study was designed to unveil the anti-nociceptive and anti-inflammatory effects of the methanolic extract of O. humifusa Raf stem (OHS).  Material and Methods: The anti-nociceptive effect was measured by hot plate, acetic acid-induced writhing, and tail flick assays in mice and rats. Moreover, the anti-inflammatory effect was measured by vascular permeability and carrageenan and serotonin-induced paw edema tests in rats. Furthermore, anti-inflammatory effect was also measured using macrophage-like LPS-induced RAW 264.7 cells. Results: OHS extract inhibited acetic acid-induced writhing (p