Circ_0000517 Contributes to Hepatocellular Carcinoma Progression by Upregulating TXNDC5 via Sponging miR-1296-5p

• Main Authors: Zang H, Li Y, Zhang X, Huang G
• Format: Article
• Language: English
• Published: Dove Medical Press 2020-05-01
• Series: Cancer Management and Research
• Subjects: hcc; circ_0000517; mir-1296-5p; txndc5
• Online Access: https://www.dovepress.com/circ0000517-contributes-to-hepatocellular-carcinoma-progression-by-upr-peer-reviewed-article-CMAR

Hongliang Zang, Yuhui Li, Xue Zhang, Guomin Huang Department of General Surgery, China-Japan Union Hospital of Jilin University, Jilin, Changchun 130012, People’s Republic of ChinaCorrespondence: Guomin HuangDepartment of General Surgery, China-Japan Union Hospital of Jilin University, No. 829 Xinmin Street, Jilin, Changchun 130012, People’s Republic of ChinaTel [phone]Email [e-mail]: Circular RNAs (circRNAs) function as essential regulators in diverse human cancers, including hepatocellular carcinoma (HCC). However, the function of circ_0000517 in HCC was unknown. We aimed to explore the roles and mechanisms of circ_0000517 in HCC.Materials and Methods: The levels of circ_0000517, RPPH1 mRNA and microRNA-1296-5p (miR-1296-5p) were measured using quantitative real-time polymerase chain reaction (qRT-PCR). The characteristics of circ_0000517 were explored by RNase R digestion and actinomycin D assays. Cell proliferation was evaluated by Cell Counting Kit-8 (CCK-8) and colony formation assays. Cell cycle process and cell apoptosis were analyzed by flow cytometry analysis. The function of circ_0000517 in vivo was explored by a murine xenograft model. The association between miR-1296-5p and circ_0000517 or thioredoxin domain containing 5 (TXNDC5) was determined by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay. The protein level of TXNDC5 was detected by Western blot assay.Results: Circ_0000517 was upregulated in HCC tissues and cells. Silencing of circ_0000517 suppressed HCC cell viability and colony formation and promoted cell cycle arrest and apoptosis in vitro and hampered tumor growth in vivo. MiR-1296-5p was a target of circ_0000517 and the effects of circ_0000517 silencing on HCC cell viability, cell cycle, colony formation and apoptosis were abolished by miR-1296-5p inhibition. TXNDC5 functioned as a target gene of miR-1296-5p, and the inhibitory effect of miR-1296-5p on HCC cell progression was rescued by TXNDC5 overexpression. Moreover, circ_0000517 promoted TXNDC5 expression via targeting miR-1296-5p.Conclusion: Circ_0000517 accelerated HCC progression by upregulating TXNDC5 through sponging miR-1296-5p.Keywords: HCC, circ_0000517, miR-1296-5p, TXNDC5