An Electrochemical DNA Sensor for p53 Tumor Suppressor Gene Detection
In this work, a sensitive electrochemical DNA sensor based on avidin modified electrode and DNA-functionalized Cds nanoparticle (DFNP) was developed. The DNA-Functionalized Cds nanoparticle contained two kinds of DNA, one was hairpin probe DNA with a biotin at the 3’’ terminal and a thiol at the 5’...
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2017-01-01
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Online Access: | https://doi.org/10.1051/matecconf/20179514008 |
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doaj-84905c564c3b4f3faa95ad96ae05d4eb2021-02-02T03:54:57ZengEDP SciencesMATEC Web of Conferences2261-236X2017-01-01951400810.1051/matecconf/20179514008matecconf_icmme2017_14008An Electrochemical DNA Sensor for p53 Tumor Suppressor Gene DetectionZhang Jie0Wan Long1Fan Hao2Liao Fusheng3Department of Pharmacy, JiangXi University of Traditional Chinese MedicineNanChang Teachers CollegeDepartment of Pharmacy, JiangXi University of Traditional Chinese MedicineDepartment of Pharmacy, JiangXi University of Traditional Chinese MedicineIn this work, a sensitive electrochemical DNA sensor based on avidin modified electrode and DNA-functionalized Cds nanoparticle (DFNP) was developed. The DNA-Functionalized Cds nanoparticle contained two kinds of DNA, one was hairpin probe DNA with a biotin at the 3’’ terminal and a thiol at the 5’ terminal, the other is linearity signal DNA. Without hybridized with target DNA, the loop of hairpin impeded biotin linked with avidin on electrode. However, after target hybridization, hairpin was opened and biotin was recognized by avidin resulting in DNA-functionalized Cds nanoparticle was brought on electrode surface. Electrochemical signals of methylene blue (MB) bound to the signal DNA were measured by differential pulse voltammetry (DPV). Introduction By using this new method, we demonstrate that this prototype sensor has been able to detect as low as picomolar p53 tumor suppressor gene with excellent differentiation ability for even single mismatches.https://doi.org/10.1051/matecconf/20179514008 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhang Jie Wan Long Fan Hao Liao Fusheng |
spellingShingle |
Zhang Jie Wan Long Fan Hao Liao Fusheng An Electrochemical DNA Sensor for p53 Tumor Suppressor Gene Detection MATEC Web of Conferences |
author_facet |
Zhang Jie Wan Long Fan Hao Liao Fusheng |
author_sort |
Zhang Jie |
title |
An Electrochemical DNA Sensor for p53 Tumor Suppressor Gene Detection |
title_short |
An Electrochemical DNA Sensor for p53 Tumor Suppressor Gene Detection |
title_full |
An Electrochemical DNA Sensor for p53 Tumor Suppressor Gene Detection |
title_fullStr |
An Electrochemical DNA Sensor for p53 Tumor Suppressor Gene Detection |
title_full_unstemmed |
An Electrochemical DNA Sensor for p53 Tumor Suppressor Gene Detection |
title_sort |
electrochemical dna sensor for p53 tumor suppressor gene detection |
publisher |
EDP Sciences |
series |
MATEC Web of Conferences |
issn |
2261-236X |
publishDate |
2017-01-01 |
description |
In this work, a sensitive electrochemical DNA sensor based on avidin modified electrode and DNA-functionalized Cds nanoparticle (DFNP) was developed. The DNA-Functionalized Cds nanoparticle contained two kinds of DNA, one was hairpin probe DNA with a biotin at the 3’’ terminal and a thiol at the 5’ terminal, the other is linearity signal DNA. Without hybridized with target DNA, the loop of hairpin impeded biotin linked with avidin on electrode. However, after target hybridization, hairpin was opened and biotin was recognized by avidin resulting in DNA-functionalized Cds nanoparticle was brought on electrode surface. Electrochemical signals of methylene blue (MB) bound to the signal DNA were measured by differential pulse voltammetry (DPV). Introduction By using this new method, we demonstrate that this prototype sensor has been able to detect as low as picomolar p53 tumor suppressor gene with excellent differentiation ability for even single mismatches. |
url |
https://doi.org/10.1051/matecconf/20179514008 |
work_keys_str_mv |
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1724306920180809728 |