SPECIFIC GENETIC FEATURES OF TUBERCULOUS MYCOBACTERIA IN URAL FEDERAL DISTRICT OF RUSSIA

• Main Authors: T. V. Umpeleva, A. A. Vyazovaya, N. I. Eremeeva, M. A. Kravchenko, O. V. Narvskaya, S. N. Skornyakov
• Format: Article
• Language: Russian
• Published: NEW TERRA Publishing House 2016-08-01
• Series: Tuberkulez i Bolezni Lëgkih
• Subjects: mycobacterium tuberculosis; miru-vntr; is6110-rflp; beijing; non-beijing; genotyping; spoligotyping
• Online Access: https://www.tibl-journal.com/jour/article/view/919

The article presents molecular genetic description of isolates of Mycobacterium tuberculosis of 178 new patients and 78 patients who had been previously treated obtained in 2009-2011 on the territory of Urals. PCR in real time and MIRU-VNTR typing of 15 loci detected prevalence of M. tuberculosis of Beijing genotype in the both groups of patients, however the part of Beijing isolates was significantly higher in the group of patients who had been previously treated compared to new patients: 80.8% versus 55.1% (p = 0.0002). IS6110-RFLP-typing showed that the majority of Beijing isolates belonged to B0 clone, clinically and epidemiologically significant in Russia. It was found out that M. tuberculosis Beijing carrying mutations rpoB Ser531→Leu and katG Ser315→Thr played the major role in MDR tuberculosis transmission in Urals. Non-Beijing group was represented by isolates of various spoligotypes and MIRU-VNTR types among which the representatives of the following globally common genetic groups prevailed: LAM (LAM9, T5_RUS), URAL (Н3), Haarlem (H1, X), Т (Т1).