S1PR2 Knockdown Promotes Migration and Invasion in Multiple Myeloma Cells via NF-κB Activation
Meng Pang,1 Chunyuan Li,1 Dong Zheng,1 Ying Wang,2 Jing Wang,1 Weilong Zhang,1 Fang Li,1 Hongmei Jing1 1Department of Hematology, Third Hospital of Peking University, Beijing, People’s Republic of China; 2Department of Immunology, Key Laboratory of Medical Immunology of Ministry of Health,...
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doaj-84b87f288c474077b62d66679eaa95c12020-11-25T03:57:24ZengDove Medical PressCancer Management and Research1179-13222020-08-01Volume 127857786556663S1PR2 Knockdown Promotes Migration and Invasion in Multiple Myeloma Cells via NF-κB ActivationPang MLi CZheng DWang YWang JZhang WLi FJing HMeng Pang,1 Chunyuan Li,1 Dong Zheng,1 Ying Wang,2 Jing Wang,1 Weilong Zhang,1 Fang Li,1 Hongmei Jing1 1Department of Hematology, Third Hospital of Peking University, Beijing, People’s Republic of China; 2Department of Immunology, Key Laboratory of Medical Immunology of Ministry of Health, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, People’s Republic of ChinaCorrespondence: Fang Li; Hongmei Jing Email lifang19611962@163.com; hongmeijing@bjmu.edu.cnBackground: The presence of circulating plasma cells (cPCs) was associated with a worse prognosis in multiple myeloma patients. However, the underlying mechanisms involved in the migration and invasion of bone marrow myeloma cells (BMMCs) to cPCs remains unclear. Here, we investigate the possible factors related to hematogenous myeloma cell dissemination and potential regulatory mechanisms.Methods: BMMCs and cPCs of five extramedullary plasmacytoma (EMP) patients were selected for single cell RNA sequencing, We found that the expression level of sphingosine-1-phosphate receptor 2 (S1RP2) was lower in cPCs compared with that in BMMCs. Then, we investigated the effect of S1PR2 in cell migration and invasion through pharmacologic inhibition with a S1PR2-selective antagonist JTE-013 or knockdown of S1PR2 expression in MM cell line U266.Results: The results showed that S1PR2 inhibition with JTE-013 or S1PR2-shRNA significantly promoted cell migration and invasion in U266 cells. We measured the expression of invasion-related proteins by Western blot and found that knockdown of S1PR2 could reduce MMP-9 expression in U266 cells. Furthermore, we found NF-κB pathway may mediate the inhibition effects of S1PR2 on cell migration and invasion in MM cells.Conclusion: Our findings demonstrated that S1PR2 downregulation may contribute to the initial extramedullary translocation by promoting cell migration and invasion through NF-κB pathway activation.Keywords: multiple myeloma, extramedullary plasmacytoma, circulating plasma cells, NF-κB, S1PR2https://www.dovepress.com/s1pr2-knockdown-promotes-migration-and-invasion-in-multiple-myeloma-ce-peer-reviewed-article-CMARmultiple myelomaextramedullary plasmacytomacirculating plasma cellss1pr2nf-κb |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pang M Li C Zheng D Wang Y Wang J Zhang W Li F Jing H |
spellingShingle |
Pang M Li C Zheng D Wang Y Wang J Zhang W Li F Jing H S1PR2 Knockdown Promotes Migration and Invasion in Multiple Myeloma Cells via NF-κB Activation Cancer Management and Research multiple myeloma extramedullary plasmacytoma circulating plasma cells s1pr2 nf-κb |
author_facet |
Pang M Li C Zheng D Wang Y Wang J Zhang W Li F Jing H |
author_sort |
Pang M |
title |
S1PR2 Knockdown Promotes Migration and Invasion in Multiple Myeloma Cells via NF-κB Activation |
title_short |
S1PR2 Knockdown Promotes Migration and Invasion in Multiple Myeloma Cells via NF-κB Activation |
title_full |
S1PR2 Knockdown Promotes Migration and Invasion in Multiple Myeloma Cells via NF-κB Activation |
title_fullStr |
S1PR2 Knockdown Promotes Migration and Invasion in Multiple Myeloma Cells via NF-κB Activation |
title_full_unstemmed |
S1PR2 Knockdown Promotes Migration and Invasion in Multiple Myeloma Cells via NF-κB Activation |
title_sort |
s1pr2 knockdown promotes migration and invasion in multiple myeloma cells via nf-κb activation |
publisher |
Dove Medical Press |
series |
Cancer Management and Research |
issn |
1179-1322 |
publishDate |
2020-08-01 |
description |
Meng Pang,1 Chunyuan Li,1 Dong Zheng,1 Ying Wang,2 Jing Wang,1 Weilong Zhang,1 Fang Li,1 Hongmei Jing1 1Department of Hematology, Third Hospital of Peking University, Beijing, People’s Republic of China; 2Department of Immunology, Key Laboratory of Medical Immunology of Ministry of Health, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, People’s Republic of ChinaCorrespondence: Fang Li; Hongmei Jing Email lifang19611962@163.com; hongmeijing@bjmu.edu.cnBackground: The presence of circulating plasma cells (cPCs) was associated with a worse prognosis in multiple myeloma patients. However, the underlying mechanisms involved in the migration and invasion of bone marrow myeloma cells (BMMCs) to cPCs remains unclear. Here, we investigate the possible factors related to hematogenous myeloma cell dissemination and potential regulatory mechanisms.Methods: BMMCs and cPCs of five extramedullary plasmacytoma (EMP) patients were selected for single cell RNA sequencing, We found that the expression level of sphingosine-1-phosphate receptor 2 (S1RP2) was lower in cPCs compared with that in BMMCs. Then, we investigated the effect of S1PR2 in cell migration and invasion through pharmacologic inhibition with a S1PR2-selective antagonist JTE-013 or knockdown of S1PR2 expression in MM cell line U266.Results: The results showed that S1PR2 inhibition with JTE-013 or S1PR2-shRNA significantly promoted cell migration and invasion in U266 cells. We measured the expression of invasion-related proteins by Western blot and found that knockdown of S1PR2 could reduce MMP-9 expression in U266 cells. Furthermore, we found NF-κB pathway may mediate the inhibition effects of S1PR2 on cell migration and invasion in MM cells.Conclusion: Our findings demonstrated that S1PR2 downregulation may contribute to the initial extramedullary translocation by promoting cell migration and invasion through NF-κB pathway activation.Keywords: multiple myeloma, extramedullary plasmacytoma, circulating plasma cells, NF-κB, S1PR2 |
topic |
multiple myeloma extramedullary plasmacytoma circulating plasma cells s1pr2 nf-κb |
url |
https://www.dovepress.com/s1pr2-knockdown-promotes-migration-and-invasion-in-multiple-myeloma-ce-peer-reviewed-article-CMAR |
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