Identification of volatile active components in Acori Tatarinowii Rhizome essential oil from different regions in China by C6 glioma cells
Abstract Background Acori Tatarinowii Rhizome (ATR) is a well-recognized Chinese herbal medicine prescribed to treat neurological disorders. The essential oil (ATEO) is considered as the active fraction of ATR and the content of ATEO is used as the only indicator for ATR content determination. The q...
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doaj-84c5eddc558c4b69b8184dadc50443fb2020-11-25T03:49:38ZengBMCBMC Complementary Medicine and Therapies2662-76712020-08-0120111310.1186/s12906-020-03020-4Identification of volatile active components in Acori Tatarinowii Rhizome essential oil from different regions in China by C6 glioma cellsLu Yan0Zhanzhan Liu1Li Xu2Yiyun Qian3Pingping Song4Min Wei5Institute of Botany, Jiangsu Province and Chinese Academy of SciencesNorth Information Control Research Academy Group Co., Ltd.State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Science, Chinese Academy SciencesInstitute of Botany, Jiangsu Province and Chinese Academy of SciencesInstitute of Botany, Jiangsu Province and Chinese Academy of SciencesInstitute of Botany, Jiangsu Province and Chinese Academy of SciencesAbstract Background Acori Tatarinowii Rhizome (ATR) is a well-recognized Chinese herbal medicine prescribed to treat neurological disorders. The essential oil (ATEO) is considered as the active fraction of ATR and the content of ATEO is used as the only indicator for ATR content determination. The quality of ATEO varies widely due to region difference; however, little is known about how to study ATEO quality chemically and biologically in response to region difference. Thus, it is of great importance to identify volatile active components in ATEO to conduct quality study. In this study, we analyzed ATEO from different regions in China using chemical component analysis combined with biological activity evaluation. Methods GC-MS was used to obtain different volatile component profiles of ATEO and significantly changed volatile components were screened out. The neuroprotective activities of ATEO, including anti-oxidation, anti-inflammation and neurotrophic functions, were revealed in C6 glioma cells. The correlation study between the bioactivities and the components was performed. Results 57 volatile components, including terpenoids, phenylpropanoids, aromatic compounds, and other aliphatic compounds, were identified. 8 volatile components (β-asarone, cis-methyl isoeugenol, γ-asarone, methyleugenol, calarene, longifolene, β-caryophyllene and caryophyllene oxide) from ATEO were significantly changed due to region difference and 2 of them (β-asarone and γ-asarone) showed strong correlation with neuroprotective activities. Conclusions Our results reveal that ATEO from different regions in China show great changes in chemical composition and biological activity. Moreover, phenylpropanoids (β-asarone and γ-asarone) present strong correlation with the bioactivities, which are considered as volatile active components in ATEO. The findings will be useful for the development of quality study of ATEO.http://link.springer.com/article/10.1186/s12906-020-03020-4Acori Tatarinowii RhizomeEssential oilRegion differenceVolatile active componentNeuroprotective |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lu Yan Zhanzhan Liu Li Xu Yiyun Qian Pingping Song Min Wei |
spellingShingle |
Lu Yan Zhanzhan Liu Li Xu Yiyun Qian Pingping Song Min Wei Identification of volatile active components in Acori Tatarinowii Rhizome essential oil from different regions in China by C6 glioma cells BMC Complementary Medicine and Therapies Acori Tatarinowii Rhizome Essential oil Region difference Volatile active component Neuroprotective |
author_facet |
Lu Yan Zhanzhan Liu Li Xu Yiyun Qian Pingping Song Min Wei |
author_sort |
Lu Yan |
title |
Identification of volatile active components in Acori Tatarinowii Rhizome essential oil from different regions in China by C6 glioma cells |
title_short |
Identification of volatile active components in Acori Tatarinowii Rhizome essential oil from different regions in China by C6 glioma cells |
title_full |
Identification of volatile active components in Acori Tatarinowii Rhizome essential oil from different regions in China by C6 glioma cells |
title_fullStr |
Identification of volatile active components in Acori Tatarinowii Rhizome essential oil from different regions in China by C6 glioma cells |
title_full_unstemmed |
Identification of volatile active components in Acori Tatarinowii Rhizome essential oil from different regions in China by C6 glioma cells |
title_sort |
identification of volatile active components in acori tatarinowii rhizome essential oil from different regions in china by c6 glioma cells |
publisher |
BMC |
series |
BMC Complementary Medicine and Therapies |
issn |
2662-7671 |
publishDate |
2020-08-01 |
description |
Abstract Background Acori Tatarinowii Rhizome (ATR) is a well-recognized Chinese herbal medicine prescribed to treat neurological disorders. The essential oil (ATEO) is considered as the active fraction of ATR and the content of ATEO is used as the only indicator for ATR content determination. The quality of ATEO varies widely due to region difference; however, little is known about how to study ATEO quality chemically and biologically in response to region difference. Thus, it is of great importance to identify volatile active components in ATEO to conduct quality study. In this study, we analyzed ATEO from different regions in China using chemical component analysis combined with biological activity evaluation. Methods GC-MS was used to obtain different volatile component profiles of ATEO and significantly changed volatile components were screened out. The neuroprotective activities of ATEO, including anti-oxidation, anti-inflammation and neurotrophic functions, were revealed in C6 glioma cells. The correlation study between the bioactivities and the components was performed. Results 57 volatile components, including terpenoids, phenylpropanoids, aromatic compounds, and other aliphatic compounds, were identified. 8 volatile components (β-asarone, cis-methyl isoeugenol, γ-asarone, methyleugenol, calarene, longifolene, β-caryophyllene and caryophyllene oxide) from ATEO were significantly changed due to region difference and 2 of them (β-asarone and γ-asarone) showed strong correlation with neuroprotective activities. Conclusions Our results reveal that ATEO from different regions in China show great changes in chemical composition and biological activity. Moreover, phenylpropanoids (β-asarone and γ-asarone) present strong correlation with the bioactivities, which are considered as volatile active components in ATEO. The findings will be useful for the development of quality study of ATEO. |
topic |
Acori Tatarinowii Rhizome Essential oil Region difference Volatile active component Neuroprotective |
url |
http://link.springer.com/article/10.1186/s12906-020-03020-4 |
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