The Expression of The Autophagy Gene Beclin-1 mRNA and Protein in Ectopic and Eutopic Endometrium of Patients with Endometriosis
Background To investigate the expression of Beclin-1 mRNA and protein in eutopic and ectopic endometrium of women with and without endometriosis, and evaluate the association of Beclin-1 protein expression and serum CA125 levels in the endometriosis group due to CA125 being a well-known biomarker of...
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doaj-84f5a64bae584a06bc8353448c26b5352020-11-25T03:57:30ZengRoyan Institute (ACECR), TehranInternational Journal of Fertility and Sterility2008-076X2008-07782015-01-018442943610.22074/ijfs.2015.418345272The Expression of The Autophagy Gene Beclin-1 mRNA and Protein in Ectopic and Eutopic Endometrium of Patients with EndometriosisLongyu Zhang0Ying Liu1Yuping Xu2Huan Wu3Zhaolian Wei4Reproductive Medicine Center, First Affiliated Hospital of Anhui Medical University, Hefei, China;Anhui No.2 Provincial People’s Hospital, ChinaReproductive Medicine Center, First Affiliated Hospital of Anhui Medical University, Hefei, ChinaReproductive Medicine Center, First Affiliated Hospital of Anhui Medical University, Hefei, ChinaReproductive Medicine Center, First Affiliated Hospital of Anhui Medical University, Hefei, ChinaReproductive Medicine Center, First Affiliated Hospital of Anhui Medical University, Hefei, ChinaBackground To investigate the expression of Beclin-1 mRNA and protein in eutopic and ectopic endometrium of women with and without endometriosis, and evaluate the association of Beclin-1 protein expression and serum CA125 levels in the endometriosis group due to CA125 being a well-known biomarker of endometriosis. Materials and Methods The expression levels (mean ± SD) of the mRNA and protein of Beclin-1 were examined in uterine endometria from 26 women without endometriosis and in eutopic and ectopic endometria from 26 endometriosis patients through experimental study, as reverse transcription PCR and Western-blotting assays. Serum CA125 levels in the endometriosis and control groups were compared and the correlation between Beclin-1 protein expression and serum CA125 was evaluated in the endometriosis group. Results Both eutopic (0.12 ± 0.04, 1.25 ± 0.42) and ectopic (0.12 ± 0.05, 1.09 ± 0.50) endometriotic tissue from 26 women with endometriosis expressed significantly lower levels of Beclin-1 mRNA and protein than endometrium from 26 normal women (0.15 ± 0.02, 1.67 ± 0.44) (p < 0.05). Serum CA125 levels were found to be significantly higher in the endometriosis group (p < 0.05). In addition, Beclin-1 protein expression of eutopic endometria in patients with endometriosis was negatively correlated with serum CA125 (r= -0.57, p < 0.01). Conclusion The present study strongly suggests that Beclin-1 may play a role in the formation and progression of endometriosis.http://www.ijfs.ir/article_45272_0b21547da5ff2ad8c9f2b7188ccc1231.pdfautophagyendometriosisbeclin1ca125 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Longyu Zhang Ying Liu Yuping Xu Huan Wu Zhaolian Wei |
spellingShingle |
Longyu Zhang Ying Liu Yuping Xu Huan Wu Zhaolian Wei The Expression of The Autophagy Gene Beclin-1 mRNA and Protein in Ectopic and Eutopic Endometrium of Patients with Endometriosis International Journal of Fertility and Sterility autophagy endometriosis beclin 1 ca125 |
author_facet |
Longyu Zhang Ying Liu Yuping Xu Huan Wu Zhaolian Wei |
author_sort |
Longyu Zhang |
title |
The Expression of The Autophagy Gene Beclin-1 mRNA and Protein in Ectopic and Eutopic Endometrium of Patients with Endometriosis |
title_short |
The Expression of The Autophagy Gene Beclin-1 mRNA and Protein in Ectopic and Eutopic Endometrium of Patients with Endometriosis |
title_full |
The Expression of The Autophagy Gene Beclin-1 mRNA and Protein in Ectopic and Eutopic Endometrium of Patients with Endometriosis |
title_fullStr |
The Expression of The Autophagy Gene Beclin-1 mRNA and Protein in Ectopic and Eutopic Endometrium of Patients with Endometriosis |
title_full_unstemmed |
The Expression of The Autophagy Gene Beclin-1 mRNA and Protein in Ectopic and Eutopic Endometrium of Patients with Endometriosis |
title_sort |
expression of the autophagy gene beclin-1 mrna and protein in ectopic and eutopic endometrium of patients with endometriosis |
publisher |
Royan Institute (ACECR), Tehran |
series |
International Journal of Fertility and Sterility |
issn |
2008-076X 2008-0778 |
publishDate |
2015-01-01 |
description |
Background To investigate the expression of Beclin-1 mRNA and protein in eutopic and ectopic endometrium of women with and without endometriosis, and evaluate the association of Beclin-1 protein expression and serum CA125 levels in the endometriosis group due to CA125 being a well-known biomarker of endometriosis. Materials and Methods The expression levels (mean ± SD) of the mRNA and protein of Beclin-1 were examined in uterine endometria from 26 women without endometriosis and in eutopic and ectopic endometria from 26 endometriosis patients through experimental study, as reverse transcription PCR and Western-blotting assays. Serum CA125 levels in the endometriosis and control groups were compared and the correlation between Beclin-1 protein expression and serum CA125 was evaluated in the endometriosis group. Results Both eutopic (0.12 ± 0.04, 1.25 ± 0.42) and ectopic (0.12 ± 0.05, 1.09 ± 0.50) endometriotic tissue from 26 women with endometriosis expressed significantly lower levels of Beclin-1 mRNA and protein than endometrium from 26 normal women (0.15 ± 0.02, 1.67 ± 0.44) (p < 0.05). Serum CA125 levels were found to be significantly higher in the endometriosis group (p < 0.05). In addition, Beclin-1 protein expression of eutopic endometria in patients with endometriosis was negatively correlated with serum CA125 (r= -0.57, p < 0.01). Conclusion The present study strongly suggests that Beclin-1 may play a role in the formation and progression of endometriosis. |
topic |
autophagy endometriosis beclin 1 ca125 |
url |
http://www.ijfs.ir/article_45272_0b21547da5ff2ad8c9f2b7188ccc1231.pdf |
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