Novel Expression of EGFL7 in Osteosarcoma and Sensitivity to Cisplatin
Epidermal growth factor-like domain 7 (EGFL7) is a protein specifically secreted by blood vessel endothelial cells, which plays an important role in angiogenesis. Considering the aberrant secretion of EGFL7 in osteosarcoma (OS) has not yet been elucidated, this study investigated the secretion of EG...
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doaj-8503c2bf81a14c34a8c305b69797964a2020-11-25T03:34:48ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2020-02-011010.3389/fonc.2020.00074511195Novel Expression of EGFL7 in Osteosarcoma and Sensitivity to CisplatinQing Liu0Qing Liu1Hongbo He2Yuhao Yuan3Hao Zeng4Zhiwei Wang5Wei Luo6Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Spine Surgery, The Second Xiangya Hospital of Central South University, Changsha, ChinaDepartment of Orthopaedics, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Orthopaedics, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Orthopaedics, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Orthopaedics, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Orthopaedics, Xiangya Hospital, Central South University, Changsha, ChinaEpidermal growth factor-like domain 7 (EGFL7) is a protein specifically secreted by blood vessel endothelial cells, which plays an important role in angiogenesis. Considering the aberrant secretion of EGFL7 in osteosarcoma (OS) has not yet been elucidated, this study investigated the secretion of EGFL7 in OS and the changes in its secretion after chemotherapy. We observed increased varying secretion of EGFL7 in OS tissues compared with chondrosarcoma (CS) tissues. OS cell lines and HUVECs showed higher EGFL7 mRNA and protein expression than SW1353, with OS cells expressing the highest levels. In patient samples, EGFL7 was highly expressed in the cytoplasm of OS tumor cells and vascular endothelium cells. This overexpression was abolished in OS cell and tumor tissues when treated with chemotherapy. This study is a pioneering study to investigate EGFL7 expression and localization in human OS tissues and cell. Overexpression of EGFL-7 in response to chemotherapy suggests that it can be used as a therapeutic target for OS.https://www.frontiersin.org/article/10.3389/fonc.2020.00074/fullepidermal growth factor-like domain 7osteosarcomaangiogenesischemotherapytherapeutic target |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qing Liu Qing Liu Hongbo He Yuhao Yuan Hao Zeng Zhiwei Wang Wei Luo |
spellingShingle |
Qing Liu Qing Liu Hongbo He Yuhao Yuan Hao Zeng Zhiwei Wang Wei Luo Novel Expression of EGFL7 in Osteosarcoma and Sensitivity to Cisplatin Frontiers in Oncology epidermal growth factor-like domain 7 osteosarcoma angiogenesis chemotherapy therapeutic target |
author_facet |
Qing Liu Qing Liu Hongbo He Yuhao Yuan Hao Zeng Zhiwei Wang Wei Luo |
author_sort |
Qing Liu |
title |
Novel Expression of EGFL7 in Osteosarcoma and Sensitivity to Cisplatin |
title_short |
Novel Expression of EGFL7 in Osteosarcoma and Sensitivity to Cisplatin |
title_full |
Novel Expression of EGFL7 in Osteosarcoma and Sensitivity to Cisplatin |
title_fullStr |
Novel Expression of EGFL7 in Osteosarcoma and Sensitivity to Cisplatin |
title_full_unstemmed |
Novel Expression of EGFL7 in Osteosarcoma and Sensitivity to Cisplatin |
title_sort |
novel expression of egfl7 in osteosarcoma and sensitivity to cisplatin |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Oncology |
issn |
2234-943X |
publishDate |
2020-02-01 |
description |
Epidermal growth factor-like domain 7 (EGFL7) is a protein specifically secreted by blood vessel endothelial cells, which plays an important role in angiogenesis. Considering the aberrant secretion of EGFL7 in osteosarcoma (OS) has not yet been elucidated, this study investigated the secretion of EGFL7 in OS and the changes in its secretion after chemotherapy. We observed increased varying secretion of EGFL7 in OS tissues compared with chondrosarcoma (CS) tissues. OS cell lines and HUVECs showed higher EGFL7 mRNA and protein expression than SW1353, with OS cells expressing the highest levels. In patient samples, EGFL7 was highly expressed in the cytoplasm of OS tumor cells and vascular endothelium cells. This overexpression was abolished in OS cell and tumor tissues when treated with chemotherapy. This study is a pioneering study to investigate EGFL7 expression and localization in human OS tissues and cell. Overexpression of EGFL-7 in response to chemotherapy suggests that it can be used as a therapeutic target for OS. |
topic |
epidermal growth factor-like domain 7 osteosarcoma angiogenesis chemotherapy therapeutic target |
url |
https://www.frontiersin.org/article/10.3389/fonc.2020.00074/full |
work_keys_str_mv |
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