Novel Expression of EGFL7 in Osteosarcoma and Sensitivity to Cisplatin

Epidermal growth factor-like domain 7 (EGFL7) is a protein specifically secreted by blood vessel endothelial cells, which plays an important role in angiogenesis. Considering the aberrant secretion of EGFL7 in osteosarcoma (OS) has not yet been elucidated, this study investigated the secretion of EG...

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Main Authors: Qing Liu, Hongbo He, Yuhao Yuan, Hao Zeng, Zhiwei Wang, Wei Luo
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-02-01
Series:Frontiers in Oncology
Subjects:
Online Access:https://www.frontiersin.org/article/10.3389/fonc.2020.00074/full
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spelling doaj-8503c2bf81a14c34a8c305b69797964a2020-11-25T03:34:48ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2020-02-011010.3389/fonc.2020.00074511195Novel Expression of EGFL7 in Osteosarcoma and Sensitivity to CisplatinQing Liu0Qing Liu1Hongbo He2Yuhao Yuan3Hao Zeng4Zhiwei Wang5Wei Luo6Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Spine Surgery, The Second Xiangya Hospital of Central South University, Changsha, ChinaDepartment of Orthopaedics, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Orthopaedics, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Orthopaedics, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Orthopaedics, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Orthopaedics, Xiangya Hospital, Central South University, Changsha, ChinaEpidermal growth factor-like domain 7 (EGFL7) is a protein specifically secreted by blood vessel endothelial cells, which plays an important role in angiogenesis. Considering the aberrant secretion of EGFL7 in osteosarcoma (OS) has not yet been elucidated, this study investigated the secretion of EGFL7 in OS and the changes in its secretion after chemotherapy. We observed increased varying secretion of EGFL7 in OS tissues compared with chondrosarcoma (CS) tissues. OS cell lines and HUVECs showed higher EGFL7 mRNA and protein expression than SW1353, with OS cells expressing the highest levels. In patient samples, EGFL7 was highly expressed in the cytoplasm of OS tumor cells and vascular endothelium cells. This overexpression was abolished in OS cell and tumor tissues when treated with chemotherapy. This study is a pioneering study to investigate EGFL7 expression and localization in human OS tissues and cell. Overexpression of EGFL-7 in response to chemotherapy suggests that it can be used as a therapeutic target for OS.https://www.frontiersin.org/article/10.3389/fonc.2020.00074/fullepidermal growth factor-like domain 7osteosarcomaangiogenesischemotherapytherapeutic target
collection DOAJ
language English
format Article
sources DOAJ
author Qing Liu
Qing Liu
Hongbo He
Yuhao Yuan
Hao Zeng
Zhiwei Wang
Wei Luo
spellingShingle Qing Liu
Qing Liu
Hongbo He
Yuhao Yuan
Hao Zeng
Zhiwei Wang
Wei Luo
Novel Expression of EGFL7 in Osteosarcoma and Sensitivity to Cisplatin
Frontiers in Oncology
epidermal growth factor-like domain 7
osteosarcoma
angiogenesis
chemotherapy
therapeutic target
author_facet Qing Liu
Qing Liu
Hongbo He
Yuhao Yuan
Hao Zeng
Zhiwei Wang
Wei Luo
author_sort Qing Liu
title Novel Expression of EGFL7 in Osteosarcoma and Sensitivity to Cisplatin
title_short Novel Expression of EGFL7 in Osteosarcoma and Sensitivity to Cisplatin
title_full Novel Expression of EGFL7 in Osteosarcoma and Sensitivity to Cisplatin
title_fullStr Novel Expression of EGFL7 in Osteosarcoma and Sensitivity to Cisplatin
title_full_unstemmed Novel Expression of EGFL7 in Osteosarcoma and Sensitivity to Cisplatin
title_sort novel expression of egfl7 in osteosarcoma and sensitivity to cisplatin
publisher Frontiers Media S.A.
series Frontiers in Oncology
issn 2234-943X
publishDate 2020-02-01
description Epidermal growth factor-like domain 7 (EGFL7) is a protein specifically secreted by blood vessel endothelial cells, which plays an important role in angiogenesis. Considering the aberrant secretion of EGFL7 in osteosarcoma (OS) has not yet been elucidated, this study investigated the secretion of EGFL7 in OS and the changes in its secretion after chemotherapy. We observed increased varying secretion of EGFL7 in OS tissues compared with chondrosarcoma (CS) tissues. OS cell lines and HUVECs showed higher EGFL7 mRNA and protein expression than SW1353, with OS cells expressing the highest levels. In patient samples, EGFL7 was highly expressed in the cytoplasm of OS tumor cells and vascular endothelium cells. This overexpression was abolished in OS cell and tumor tissues when treated with chemotherapy. This study is a pioneering study to investigate EGFL7 expression and localization in human OS tissues and cell. Overexpression of EGFL-7 in response to chemotherapy suggests that it can be used as a therapeutic target for OS.
topic epidermal growth factor-like domain 7
osteosarcoma
angiogenesis
chemotherapy
therapeutic target
url https://www.frontiersin.org/article/10.3389/fonc.2020.00074/full
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AT yuhaoyuan novelexpressionofegfl7inosteosarcomaandsensitivitytocisplatin
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