Structure changes of retina and expression of TGF-β<sub>2</sub> in the retina in form deprivation myopia of guinea pigs
AIM: To observe the structure changes of retina and determine the expression of TGF-β<sub>2</sub> in retina model. At the same time to clarify the function of TGF-β<sub>2</sub> in form deprivation myopia model. <p>METHODS: A facemask was worn on the right eyes of guinea...
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doaj-85297def21ad470796853d11a13f7a9d2020-11-25T01:29:48ZengPress of International Journal of Ophthalmology (IJO PRESS)Guoji Yanke Zazhi1672-51231672-51232014-11-0114111950195210.3980/j.issn.1672-5123.2014.11.09Structure changes of retina and expression of TGF-β<sub>2</sub> in the retina in form deprivation myopia of guinea pigsLing-Xiao Zhou0Li-Lun Wang1Lin Zhang2Department of Ophthalmology, the First Affiliated Hospital of Xi'an Medical University, Xi'an 710077, Shaanxi Province, ChinaDepartment of Ophthalmology, Yan'an University Affiliated Hospital, Yan'an 716000, Shaanxi Province, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, ChinaAIM: To observe the structure changes of retina and determine the expression of TGF-β<sub>2</sub> in retina model. At the same time to clarify the function of TGF-β<sub>2</sub> in form deprivation myopia model. <p>METHODS: A facemask was worn on the right eyes of guinea pigs to develop form deprivation myopia. Before and after experiment, refraction was measured using retinoscope, and ocular axial was determined by A-scan ultrasonography. Morphologic alternations of the retina were observed by light microsope. The expression of TGF-β<sub>2</sub> in the retina was determined by immunohistochemistry.<p>RESULTS:Retina of myopic eyes became thinner, and inner nuclear layer of retina had few layers, nuclear of outer nuclear layer became smaller and circular, at the same time, they arranged in disorder. The immunohistochemisty results showed that expression of TGF-β<sub>2</sub> in model eyes was lower than that in control eyes(<i>P</i><0.05).<p>CONCLUSION: Covering eyes with facemasks is effective, simple and convenient in making myopic model. The retina of the model eye appeares degeneration. TGF-β<sub>2</sub> plays an important role in the course of development in form deprivation myopia.http://ies.ijo.cn/cn_publish/2014/11/201411009.pdfform deprivation myopiaretinaTGF-β<sub>2</sub> |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ling-Xiao Zhou Li-Lun Wang Lin Zhang |
spellingShingle |
Ling-Xiao Zhou Li-Lun Wang Lin Zhang Structure changes of retina and expression of TGF-β<sub>2</sub> in the retina in form deprivation myopia of guinea pigs Guoji Yanke Zazhi form deprivation myopia retina TGF-β<sub>2</sub> |
author_facet |
Ling-Xiao Zhou Li-Lun Wang Lin Zhang |
author_sort |
Ling-Xiao Zhou |
title |
Structure changes of retina and expression of TGF-β<sub>2</sub> in the retina in form deprivation myopia of guinea pigs |
title_short |
Structure changes of retina and expression of TGF-β<sub>2</sub> in the retina in form deprivation myopia of guinea pigs |
title_full |
Structure changes of retina and expression of TGF-β<sub>2</sub> in the retina in form deprivation myopia of guinea pigs |
title_fullStr |
Structure changes of retina and expression of TGF-β<sub>2</sub> in the retina in form deprivation myopia of guinea pigs |
title_full_unstemmed |
Structure changes of retina and expression of TGF-β<sub>2</sub> in the retina in form deprivation myopia of guinea pigs |
title_sort |
structure changes of retina and expression of tgf-β<sub>2</sub> in the retina in form deprivation myopia of guinea pigs |
publisher |
Press of International Journal of Ophthalmology (IJO PRESS) |
series |
Guoji Yanke Zazhi |
issn |
1672-5123 1672-5123 |
publishDate |
2014-11-01 |
description |
AIM: To observe the structure changes of retina and determine the expression of TGF-β<sub>2</sub> in retina model. At the same time to clarify the function of TGF-β<sub>2</sub> in form deprivation myopia model. <p>METHODS: A facemask was worn on the right eyes of guinea pigs to develop form deprivation myopia. Before and after experiment, refraction was measured using retinoscope, and ocular axial was determined by A-scan ultrasonography. Morphologic alternations of the retina were observed by light microsope. The expression of TGF-β<sub>2</sub> in the retina was determined by immunohistochemistry.<p>RESULTS:Retina of myopic eyes became thinner, and inner nuclear layer of retina had few layers, nuclear of outer nuclear layer became smaller and circular, at the same time, they arranged in disorder. The immunohistochemisty results showed that expression of TGF-β<sub>2</sub> in model eyes was lower than that in control eyes(<i>P</i><0.05).<p>CONCLUSION: Covering eyes with facemasks is effective, simple and convenient in making myopic model. The retina of the model eye appeares degeneration. TGF-β<sub>2</sub> plays an important role in the course of development in form deprivation myopia. |
topic |
form deprivation myopia retina TGF-β<sub>2</sub> |
url |
http://ies.ijo.cn/cn_publish/2014/11/201411009.pdf |
work_keys_str_mv |
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