Novel in vitro and in vivo anti-Helicobacter pylori effects of pomegranate peel ethanol extract
Background and Aim: Interest in plants with antimicrobial properties has been revived due to emerging problems associated with using antibiotics to eradicate Helicobacter pylori. Accordingly, this study aims to assess the antibacterial effects of Punica granatum and the possible synergistic effect o...
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doaj-852de48520174f61815a6581abf1e1512021-08-02T15:31:19ZengVeterinary WorldVeterinary World0972-89882231-09162021-01-0114112012810.14202/vetworld.2021.120-128Novel in vitro and in vivo anti-Helicobacter pylori effects of pomegranate peel ethanol extractAmal Mayyas0https://orcid.org/0000-0002-5036-7586Mohammad Abu-Sini1https://orcid.org/0000-0001-7352-9739Rula Amr2https://orcid.org/0000-0001-5514-793XRand T. Akasheh3https://orcid.org/0000-0002-0018-0342Waleed Zalloum4https://orcid.org/0000-0001-5599-6325Ayman Khdair5https://orcid.org/0000-0002-6314-105XIslam Hamad6https://orcid.org/0000-0003-4326-8624Talal Aburjai7https://orcid.org/0000-0002-6155-7975Rula M. Darwish8https://orcid.org/0000-0003-2359-676XLuay Abu-Qatouseh9https://orcid.org/0000-0003-1551-2343Department of Pharmaceutical Sciences, Faculty of Pharmacy, University of Jordan, 11914 Amman, Jordan; Department of Pharmacy, Faculty of Health Sciences, American University of Madaba, 11821 Madaba, Jordan.Department of Pharmacy, Faculty of Pharmacy, Al-Zaytoonah University of Jordan, Amman, Jordan.Department of Pharmacy, Faculty of Health Sciences, American University of Madaba, 11821 Madaba, Jordan.Department of Pharmacy, Faculty of Health Sciences, American University of Madaba, 11821 Madaba, Jordan.Department of Pharmacy, Faculty of Health Sciences, American University of Madaba, 11821 Madaba, Jordan.Department of Pharmacy, Faculty of Health Sciences, American University of Madaba, 11821 Madaba, Jordan.Department of Pharmacy, Faculty of Health Sciences, American University of Madaba, 11821 Madaba, Jordan.Department of Pharmaceutical Sciences, Faculty of Pharmacy, University of Jordan, 11914 Amman, Jordan.Department of Pharmaceutical Sciences, Faculty of Pharmacy, University of Jordan, 11914 Amman, Jordan.Department of Pharmacology and Biomedical Sciences, Faculty of Pharmacy, University of Petra, 961343 Amman, Jordan.Background and Aim: Interest in plants with antimicrobial properties has been revived due to emerging problems associated with using antibiotics to eradicate Helicobacter pylori. Accordingly, this study aims to assess the antibacterial effects of Punica granatum and the possible synergistic effect of its extract along with metronidazole against H. pylori. Materials and Methods: Pomegranate peel ethanol extracts (PPEE) was tested against a control strain of H. pylori (NCTC 11916) in vitro and in vivo in female Wistar rats. Moreover, the synergistic effect of PPEE in combination with metronidazole was tested in vitro. Results: The PPEE exhibited a remarkable activity against H. pylori with a minimum inhibitory concentration (MIC) of 0.156 mg/mL. Furthermore, the extract exhibited a pronounced urease inhibitory activity (IC50 ∼6 mg/mL) against the tested strain. A synergistic effect between PPEE and metronidazole was also observed (fractional inhibitory concentrations <0.5). Oral treatment of rats with PPEE for 8 days produced a significant reduction in H. pylori gastritis and a significant decrease in both lymphocytic and positive chronicity. Conclusion: Pomegranate extract is probably safe and represents a potential alternative and complementary therapy for reducing H. pylori associated with gastric ulcers.http://www.veterinaryworld.org/Vol.14/January-2021/16.pdfhelicobacter pyloriin vitroin vivopomegranate peel ethanol extractsurease inhibition |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Amal Mayyas Mohammad Abu-Sini Rula Amr Rand T. Akasheh Waleed Zalloum Ayman Khdair Islam Hamad Talal Aburjai Rula M. Darwish Luay Abu-Qatouseh |
spellingShingle |
Amal Mayyas Mohammad Abu-Sini Rula Amr Rand T. Akasheh Waleed Zalloum Ayman Khdair Islam Hamad Talal Aburjai Rula M. Darwish Luay Abu-Qatouseh Novel in vitro and in vivo anti-Helicobacter pylori effects of pomegranate peel ethanol extract Veterinary World helicobacter pylori in vitro in vivo pomegranate peel ethanol extracts urease inhibition |
author_facet |
Amal Mayyas Mohammad Abu-Sini Rula Amr Rand T. Akasheh Waleed Zalloum Ayman Khdair Islam Hamad Talal Aburjai Rula M. Darwish Luay Abu-Qatouseh |
author_sort |
Amal Mayyas |
title |
Novel in vitro and in vivo anti-Helicobacter pylori effects of pomegranate peel ethanol extract |
title_short |
Novel in vitro and in vivo anti-Helicobacter pylori effects of pomegranate peel ethanol extract |
title_full |
Novel in vitro and in vivo anti-Helicobacter pylori effects of pomegranate peel ethanol extract |
title_fullStr |
Novel in vitro and in vivo anti-Helicobacter pylori effects of pomegranate peel ethanol extract |
title_full_unstemmed |
Novel in vitro and in vivo anti-Helicobacter pylori effects of pomegranate peel ethanol extract |
title_sort |
novel in vitro and in vivo anti-helicobacter pylori effects of pomegranate peel ethanol extract |
publisher |
Veterinary World |
series |
Veterinary World |
issn |
0972-8988 2231-0916 |
publishDate |
2021-01-01 |
description |
Background and Aim: Interest in plants with antimicrobial properties has been revived due to emerging problems associated with using antibiotics to eradicate Helicobacter pylori. Accordingly, this study aims to assess the antibacterial effects of Punica granatum and the possible synergistic effect of its extract along with metronidazole against H. pylori.
Materials and Methods: Pomegranate peel ethanol extracts (PPEE) was tested against a control strain of H. pylori (NCTC 11916) in vitro and in vivo in female Wistar rats. Moreover, the synergistic effect of PPEE in combination with metronidazole was tested in vitro.
Results: The PPEE exhibited a remarkable activity against H. pylori with a minimum inhibitory concentration (MIC) of 0.156 mg/mL. Furthermore, the extract exhibited a pronounced urease inhibitory activity (IC50 ∼6 mg/mL) against the tested strain. A synergistic effect between PPEE and metronidazole was also observed (fractional inhibitory concentrations <0.5). Oral treatment of rats with PPEE for 8 days produced a significant reduction in H. pylori gastritis and a significant decrease in both lymphocytic and positive chronicity.
Conclusion: Pomegranate extract is probably safe and represents a potential alternative and complementary therapy for reducing H. pylori associated with gastric ulcers. |
topic |
helicobacter pylori in vitro in vivo pomegranate peel ethanol extracts urease inhibition |
url |
http://www.veterinaryworld.org/Vol.14/January-2021/16.pdf |
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