Marker-assisted screening of breeding populations of an apomictic grass Cenchrus ciliaris L. segregating for the mode of reproduction
Cenchrus ciliaris L. is an apomictic forage grass grown in pastures and rangelands of the semi-arid tropics. It reproduces predominantly through apomixis; rarely, obligate sexual plants have also been reported. Absence of sexual reproduction limits the possibility of genetic improvement through hybr...
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Brazilian Society of Plant Breeding
2016-12-01
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doaj-853115f32c7443d59598dacc3bd676f42020-11-25T01:29:17ZengBrazilian Society of Plant BreedingCrop Breeding and Applied Biotechnology1518-78531518-78532016-12-01171101710.1590/1984- 70332017v17n1a2Marker-assisted screening of breeding populations of an apomictic grass Cenchrus ciliaris L. segregating for the mode of reproductionSuresh Kumar0Sheena Saxena1Madan G. Gupta2 ICAR - Indian Grassland and Fodder Research Institute, Division of Crop Improvement ICAR - Indian Grassland and Fodder Research Institute, Division of Crop Improvement ICAR - Indian Grassland and Fodder Research Institute, Division of Crop ImprovementCenchrus ciliaris L. is an apomictic forage grass grown in pastures and rangelands of the semi-arid tropics. It reproduces predominantly through apomixis; rarely, obligate sexual plants have also been reported. Absence of sexual reproduction limits the possibility of genetic improvement through hybridization. This study reports on hybridization of an obligate sexual, self- incompatible buffelgrass with pollen from apomictic plants towards development of an F2 population segregating for mode of reproduction and use of sequence characterized amplified region (SCAR) markers for screening the population. The segregation ratio of 3:1 (facultative: apomictic) was observed in the F1 generation, whereas it was 1:2:1 (apomictic: facultative: sexual) in the F2 generation. A number of obligate sexual F2 progenies with desirable agronomic traits were obtained. The SCAR markers were able to screen out apomictic plants from sexual ones, but failed to discriminate between facultative and sexual. Marker-assisted screening could be useful for introgression of desirable trait(s) in the apomictic genotype through hybridization.http://www.sbmp.org.br/cbab/siscbab/uploads/c8eb9792-0338-bd53.pdfSCARapomixismolecular marker |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Suresh Kumar Sheena Saxena Madan G. Gupta |
spellingShingle |
Suresh Kumar Sheena Saxena Madan G. Gupta Marker-assisted screening of breeding populations of an apomictic grass Cenchrus ciliaris L. segregating for the mode of reproduction Crop Breeding and Applied Biotechnology SCAR apomixis molecular marker |
author_facet |
Suresh Kumar Sheena Saxena Madan G. Gupta |
author_sort |
Suresh Kumar |
title |
Marker-assisted screening of breeding populations of an apomictic grass Cenchrus ciliaris L. segregating for the mode of reproduction |
title_short |
Marker-assisted screening of breeding populations of an apomictic grass Cenchrus ciliaris L. segregating for the mode of reproduction |
title_full |
Marker-assisted screening of breeding populations of an apomictic grass Cenchrus ciliaris L. segregating for the mode of reproduction |
title_fullStr |
Marker-assisted screening of breeding populations of an apomictic grass Cenchrus ciliaris L. segregating for the mode of reproduction |
title_full_unstemmed |
Marker-assisted screening of breeding populations of an apomictic grass Cenchrus ciliaris L. segregating for the mode of reproduction |
title_sort |
marker-assisted screening of breeding populations of an apomictic grass cenchrus ciliaris l. segregating for the mode of reproduction |
publisher |
Brazilian Society of Plant Breeding |
series |
Crop Breeding and Applied Biotechnology |
issn |
1518-7853 1518-7853 |
publishDate |
2016-12-01 |
description |
Cenchrus ciliaris L. is an apomictic forage grass grown in pastures and rangelands of the semi-arid tropics. It reproduces predominantly through apomixis; rarely, obligate sexual plants have also been reported. Absence of sexual reproduction limits the possibility of genetic improvement through hybridization. This study reports on hybridization of an obligate sexual, self-
incompatible buffelgrass with pollen from apomictic plants towards development of an F2 population segregating for mode of reproduction and use of sequence characterized amplified region (SCAR) markers for screening the population. The segregation ratio of 3:1 (facultative: apomictic) was observed in the F1 generation, whereas it was 1:2:1 (apomictic: facultative: sexual) in the F2 generation. A number of obligate sexual F2 progenies with desirable agronomic traits were obtained. The SCAR markers were able to screen out apomictic plants from sexual ones, but failed to discriminate between facultative and sexual. Marker-assisted screening could be useful for introgression of desirable trait(s) in the apomictic genotype through hybridization. |
topic |
SCAR apomixis molecular marker |
url |
http://www.sbmp.org.br/cbab/siscbab/uploads/c8eb9792-0338-bd53.pdf |
work_keys_str_mv |
AT sureshkumar markerassistedscreeningofbreedingpopulationsofanapomicticgrasscenchrusciliarislsegregatingforthemodeofreproduction AT sheenasaxena markerassistedscreeningofbreedingpopulationsofanapomicticgrasscenchrusciliarislsegregatingforthemodeofreproduction AT madanggupta markerassistedscreeningofbreedingpopulationsofanapomicticgrasscenchrusciliarislsegregatingforthemodeofreproduction |
_version_ |
1725097212692660224 |