EFFECT OF THE ORTHOSIPHON STAMINEUS, BENTH ON AMINOPYRINE METABOLISM IN RAT HEPATOCYTES

• Main Authors: CHIN JIN HAN1, ABAS HJ. HUSSIN
• Format: Article
• Language: English
• Published: Universiti Sains Malaysia 2007-01-01
• Series: Malaysian Journal of Pharmaceutical Sciences
• Subjects: Orthosiphon stamineus; Aminopyrine; Hepatocytes; Protein kinases
• Online Access: http://www.usm.my/mjps/MJPS%205%20(1)%202007/MJPS%205.1.4.pdf

One of the commonly used herbal medicines in Malaysia is Orthosiphon stamineus, Benth (family: Lamiaceae) or locally known as Misai Kucing. This experiment was undertaken to evaluate possible interaction of methanol extract of O. stamineus with aminopyrine, a model drug, in different age groups (young, adult and old) of Sprague-Dawley (SD) female rat hepatocytes. Hepatocytes were prepared by collagenase perfusion technique. Determination of aminopyrine N-demethylase activity was done by measuring formaldehyde formed. From these findings, only normal young female rat hepatocytes in the presence of 0.001 mg/ml of methanol extract of O. stamineus showed significant increase in aminopyrine N-demethylase activity. However, aminopyrine N-demethylase activity was not affected in hepatocytes of normal adult and old female SD rats. In conclusion, exposure of methanol extract of O. stamineus could affect phase I aminopyrine metabolism in normal young female SD rat hepatocytes but this effect was age-dependent.