Towards a structural comprehension of bacterial type VI secretion systems: characterization of the TssJ-TssM complex of an Escherichia coli pathovar.
Type VI secretion systems (T6SS) are trans-envelope machines dedicated to the secretion of virulence factors into eukaryotic or prokaryotic cells, therefore required for pathogenesis and/or for competition towards neighboring bacteria. The T6SS apparatus resembles the injection device of bacteriopha...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2011-11-01
|
Series: | PLoS Pathogens |
Online Access: | http://europepmc.org/articles/PMC3213119?pdf=render |
id |
doaj-85565310bbf5449eae0b500910e742f9 |
---|---|
record_format |
Article |
spelling |
doaj-85565310bbf5449eae0b500910e742f92020-11-25T00:12:15ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742011-11-01711e100238610.1371/journal.ppat.1002386Towards a structural comprehension of bacterial type VI secretion systems: characterization of the TssJ-TssM complex of an Escherichia coli pathovar.Catarina Felisberto-RodriguesEric DurandMarie-Stéphanie AschtgenStéphanie BlangyMiguel Ortiz-LombardiaBadreddine DouziChristian CambillauEric CascalesType VI secretion systems (T6SS) are trans-envelope machines dedicated to the secretion of virulence factors into eukaryotic or prokaryotic cells, therefore required for pathogenesis and/or for competition towards neighboring bacteria. The T6SS apparatus resembles the injection device of bacteriophage T4, and is anchored to the cell envelope through a membrane complex. This membrane complex is composed of the TssL, TssM and TagL inner membrane anchored proteins and of the TssJ outer membrane lipoprotein. Here, we report the crystal structure of the enteroaggregative Escherichia coli Sci1 TssJ lipoprotein, a two four-stranded β-sheets protein that exhibits a transthyretin fold with an additional α-helical domain and a protruding loop. We showed that TssJ contacts TssM through this loop since a loop depleted mutant failed to interact with TssM in vitro or in vivo. Biophysical analysis of TssM and TssJ-TssM interaction suggest a structural model of the membrane-anchored outer shell of T6SS. Collectively, our results provide an improved understanding of T6SS assembly and encourage structure-aided drug design of novel antimicrobials targeting T6SS.http://europepmc.org/articles/PMC3213119?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Catarina Felisberto-Rodrigues Eric Durand Marie-Stéphanie Aschtgen Stéphanie Blangy Miguel Ortiz-Lombardia Badreddine Douzi Christian Cambillau Eric Cascales |
spellingShingle |
Catarina Felisberto-Rodrigues Eric Durand Marie-Stéphanie Aschtgen Stéphanie Blangy Miguel Ortiz-Lombardia Badreddine Douzi Christian Cambillau Eric Cascales Towards a structural comprehension of bacterial type VI secretion systems: characterization of the TssJ-TssM complex of an Escherichia coli pathovar. PLoS Pathogens |
author_facet |
Catarina Felisberto-Rodrigues Eric Durand Marie-Stéphanie Aschtgen Stéphanie Blangy Miguel Ortiz-Lombardia Badreddine Douzi Christian Cambillau Eric Cascales |
author_sort |
Catarina Felisberto-Rodrigues |
title |
Towards a structural comprehension of bacterial type VI secretion systems: characterization of the TssJ-TssM complex of an Escherichia coli pathovar. |
title_short |
Towards a structural comprehension of bacterial type VI secretion systems: characterization of the TssJ-TssM complex of an Escherichia coli pathovar. |
title_full |
Towards a structural comprehension of bacterial type VI secretion systems: characterization of the TssJ-TssM complex of an Escherichia coli pathovar. |
title_fullStr |
Towards a structural comprehension of bacterial type VI secretion systems: characterization of the TssJ-TssM complex of an Escherichia coli pathovar. |
title_full_unstemmed |
Towards a structural comprehension of bacterial type VI secretion systems: characterization of the TssJ-TssM complex of an Escherichia coli pathovar. |
title_sort |
towards a structural comprehension of bacterial type vi secretion systems: characterization of the tssj-tssm complex of an escherichia coli pathovar. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Pathogens |
issn |
1553-7366 1553-7374 |
publishDate |
2011-11-01 |
description |
Type VI secretion systems (T6SS) are trans-envelope machines dedicated to the secretion of virulence factors into eukaryotic or prokaryotic cells, therefore required for pathogenesis and/or for competition towards neighboring bacteria. The T6SS apparatus resembles the injection device of bacteriophage T4, and is anchored to the cell envelope through a membrane complex. This membrane complex is composed of the TssL, TssM and TagL inner membrane anchored proteins and of the TssJ outer membrane lipoprotein. Here, we report the crystal structure of the enteroaggregative Escherichia coli Sci1 TssJ lipoprotein, a two four-stranded β-sheets protein that exhibits a transthyretin fold with an additional α-helical domain and a protruding loop. We showed that TssJ contacts TssM through this loop since a loop depleted mutant failed to interact with TssM in vitro or in vivo. Biophysical analysis of TssM and TssJ-TssM interaction suggest a structural model of the membrane-anchored outer shell of T6SS. Collectively, our results provide an improved understanding of T6SS assembly and encourage structure-aided drug design of novel antimicrobials targeting T6SS. |
url |
http://europepmc.org/articles/PMC3213119?pdf=render |
work_keys_str_mv |
AT catarinafelisbertorodrigues towardsastructuralcomprehensionofbacterialtypevisecretionsystemscharacterizationofthetssjtssmcomplexofanescherichiacolipathovar AT ericdurand towardsastructuralcomprehensionofbacterialtypevisecretionsystemscharacterizationofthetssjtssmcomplexofanescherichiacolipathovar AT mariestephanieaschtgen towardsastructuralcomprehensionofbacterialtypevisecretionsystemscharacterizationofthetssjtssmcomplexofanescherichiacolipathovar AT stephanieblangy towardsastructuralcomprehensionofbacterialtypevisecretionsystemscharacterizationofthetssjtssmcomplexofanescherichiacolipathovar AT miguelortizlombardia towardsastructuralcomprehensionofbacterialtypevisecretionsystemscharacterizationofthetssjtssmcomplexofanescherichiacolipathovar AT badreddinedouzi towardsastructuralcomprehensionofbacterialtypevisecretionsystemscharacterizationofthetssjtssmcomplexofanescherichiacolipathovar AT christiancambillau towardsastructuralcomprehensionofbacterialtypevisecretionsystemscharacterizationofthetssjtssmcomplexofanescherichiacolipathovar AT ericcascales towardsastructuralcomprehensionofbacterialtypevisecretionsystemscharacterizationofthetssjtssmcomplexofanescherichiacolipathovar |
_version_ |
1725400187520679936 |