Transporter Molecules influence the Gene Expression in HeLa Cells

• Main Author: Waldemar Waldeck, Ruediger Pipkorn, Bernhard Korn, Gabriele Mueller, Matthias Schick, Katalin Tóth, Manfred Wiessler, Bernd Didinger, Klaus Braun
• Format: Article
• Language: English
• Published: Ivyspring International Publisher 2009-01-01
• Series: International Journal of Medical Sciences
• Online Access: http://www.medsci.org/v06p0018.htm

<p>Progresses in biology and pharmacology led to highly specific bioactive substances, but their poor bioavailability at the site of action is a result of their physico-chemical properties. Various design approaches for transport carrier molecules facilitating the cellular entry of bioactive substances could help to reach their molecular target in cells and tissues. The transfer efficacy and the subsequent pharmacological effects of the cargo molecules are well investigated, but the investigations of effects of the carrier molecules themselves on the target cells or tissues remain necessary. A special attention should be paid to the differential gene expression, particularly in the interpretation of the data achieved by highly specific active pharmaceutical products. After application of transmembrane transport peptides, particularly the pAnt and also the HIV-1 Tat, cells respond with a conspicuous altered gene expression of at least three genes. The <i>PKN1 </i>gene was induced and two genes (<i>ZCD1 </i>and <i>BSG</i>) were slightly repressed. The genes and the chromosomes are described, the moderate differential gene expression graphed, and the ontology is listed.</p>