| Summary: | <i>Beauveria bassiana</i> is an entomopathogenic fungus that is used for the biological control of different agricultural pest insects. <i>B. bassiana</i> is traditionally cultivated in submerged fermentation and solid-state fermentation systems to obtain secondary metabolites with antifungal activity and infective spores. This work presents the design and characterization of a new laboratory-scale biofilm bioreactor for the simultaneous production of oosporein and aerial conidia by <i>B. bassiana</i> PQ2. The reactor was built with materials available in a conventional laboratory. <i>K<sub>L</sub>a</i> was determined at different air flows (1.5–2.5 L/min) by two different methods in the liquid phase and in the exhaust gases. The obtained values showed that an air flow of 2.5 L/min is sufficient to ensure adequate aeration to produce aerial conidia and secondary metabolites by <i>B. bassiana</i>. Under the conditions studied, a concentration of 183 mg oosporein per liter and 1.24 × 10<sup>9</sup> spores per gram of support was obtained at 168 h of culture. These results indicate that the biofilm bioreactor represents a viable alternative for the production of products for biological control from <i>B. bassiana</i>.
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