Polydopamine Linking Substrate for AMPs: Characterisation and Stability on Ti6Al4V

Polydopamine Linking Substrate for AMPs: Characterisation and Stability on Ti6Al4V

Infections are common complications in joint replacement surgeries. Eradicated infections can lead to implant failure. In this paper, analogues of the peptide KR-12 derived from the human cathelicidin LL-37 were designed, synthesised, and characterised. The designed antimicrobial peptides (AMPs) wer...

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Main Authors: Zuzanna Trzcińska, Marc Bruggeman, Hanieh Ijakipour, Nikolas J. Hodges, James Bowen, Artemis Stamboulis
Format: Article
Language:English
Published: MDPI AG 2020-08-01
Series:Materials
Subjects:
Ti6Al4V
polydopamine
antimicrobial peptides
cathelicidin
KR-12
Online Access:https://www.mdpi.com/1996-1944/13/17/3714
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spelling doaj-8583907bba3c42bf999125aaa36d1f372020-11-25T03:39:29ZengMDPI AGMaterials1996-19442020-08-01133714371410.3390/ma13173714Polydopamine Linking Substrate for AMPs: Characterisation and Stability on Ti6Al4VZuzanna Trzcińska0Marc Bruggeman1Hanieh Ijakipour2Nikolas J. Hodges3James Bowen4Artemis Stamboulis5School of Metallurgy and Materials, University of Birmingham, Edgbaston, Birmingham B15 2TT, UKSchool of Metallurgy and Materials, University of Birmingham, Edgbaston, Birmingham B15 2TT, UKSchool of Metallurgy and Materials, University of Birmingham, Edgbaston, Birmingham B15 2TT, UKSchool of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UKSchool of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham B15 2TT, UKSchool of Metallurgy and Materials, University of Birmingham, Edgbaston, Birmingham B15 2TT, UKInfections are common complications in joint replacement surgeries. Eradicated infections can lead to implant failure. In this paper, analogues of the peptide KR-12 derived from the human cathelicidin LL-37 were designed, synthesised, and characterised. The designed antimicrobial peptides (AMPs) were attached to the surface of a titanium alloy, Ti6Al4V, by conjugation to a polydopamine linking substrate. The topography of the polydopamine coating was evaluated by electron microscopy and coating thickness measurements were performed with ellipsometry and Atomic Force Microscopy (AFM). The subsequently attached peptide stability was investigated with release profile studies in simulated body fluid, using both fluorescence imaging and High-Performance Liquid Chromatography (HPLC). Finally, the hydrophobicity of the coating was characterised by water contact angle measurements. The designed AMPs were shown to provide long-term bonding to the polydopamine-coated Ti6Al4V surfaces.https://www.mdpi.com/1996-1944/13/17/3714Ti6Al4Vpolydopamineantimicrobial peptidescathelicidinKR-12
collection DOAJ
language English
format Article
sources DOAJ
author Zuzanna Trzcińska
Marc Bruggeman
Hanieh Ijakipour
Nikolas J. Hodges
James Bowen
Artemis Stamboulis
spellingShingle Zuzanna Trzcińska
Marc Bruggeman
Hanieh Ijakipour
Nikolas J. Hodges
James Bowen
Artemis Stamboulis
Polydopamine Linking Substrate for AMPs: Characterisation and Stability on Ti6Al4V
Materials
Ti6Al4V
polydopamine
antimicrobial peptides
cathelicidin
KR-12
author_facet Zuzanna Trzcińska
Marc Bruggeman
Hanieh Ijakipour
Nikolas J. Hodges
James Bowen
Artemis Stamboulis
author_sort Zuzanna Trzcińska
title Polydopamine Linking Substrate for AMPs: Characterisation and Stability on Ti6Al4V
title_short Polydopamine Linking Substrate for AMPs: Characterisation and Stability on Ti6Al4V
title_full Polydopamine Linking Substrate for AMPs: Characterisation and Stability on Ti6Al4V
title_fullStr Polydopamine Linking Substrate for AMPs: Characterisation and Stability on Ti6Al4V
title_full_unstemmed Polydopamine Linking Substrate for AMPs: Characterisation and Stability on Ti6Al4V
title_sort polydopamine linking substrate for amps: characterisation and stability on ti6al4v
publisher MDPI AG
series Materials
issn 1996-1944
publishDate 2020-08-01
description Infections are common complications in joint replacement surgeries. Eradicated infections can lead to implant failure. In this paper, analogues of the peptide KR-12 derived from the human cathelicidin LL-37 were designed, synthesised, and characterised. The designed antimicrobial peptides (AMPs) were attached to the surface of a titanium alloy, Ti6Al4V, by conjugation to a polydopamine linking substrate. The topography of the polydopamine coating was evaluated by electron microscopy and coating thickness measurements were performed with ellipsometry and Atomic Force Microscopy (AFM). The subsequently attached peptide stability was investigated with release profile studies in simulated body fluid, using both fluorescence imaging and High-Performance Liquid Chromatography (HPLC). Finally, the hydrophobicity of the coating was characterised by water contact angle measurements. The designed AMPs were shown to provide long-term bonding to the polydopamine-coated Ti6Al4V surfaces.
topic Ti6Al4V
polydopamine
antimicrobial peptides
cathelicidin
KR-12
url https://www.mdpi.com/1996-1944/13/17/3714
work_keys_str_mv AT zuzannatrzcinska polydopaminelinkingsubstrateforampscharacterisationandstabilityonti6al4v
AT marcbruggeman polydopaminelinkingsubstrateforampscharacterisationandstabilityonti6al4v
AT haniehijakipour polydopaminelinkingsubstrateforampscharacterisationandstabilityonti6al4v
AT nikolasjhodges polydopaminelinkingsubstrateforampscharacterisationandstabilityonti6al4v
AT jamesbowen polydopaminelinkingsubstrateforampscharacterisationandstabilityonti6al4v
AT artemisstamboulis polydopaminelinkingsubstrateforampscharacterisationandstabilityonti6al4v
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