2-(Dimethylamino)ethyl Methacrylate/(2-Hydroxyethyl) Methacrylate/α-Tricalcium Phosphate Cryogels for Bone Repair, Preparation and Evaluation of the Biological Response of Human Trabecular Bone-Derived Cells and Mesenchymal Stem Cells
The aim of this work is to evaluate the potential of cryogels to be used as scaffolds in tissue engineering. Scaffolds based on the α-tricalcium phosphate reinforced PDMAEMA (Poly(dimethyl aminoethyl methacrylate))/PHEMA (poly(hydroxyethyl methacrylate)) system were prepared and human trabecular bon...
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doaj-8597875e4c7c49ba983ec91a688c31fb2020-11-24T21:06:44ZengMDPI AGPolymers2073-43602014-09-016102510252510.3390/polym6102510polym61025102-(Dimethylamino)ethyl Methacrylate/(2-Hydroxyethyl) Methacrylate/α-Tricalcium Phosphate Cryogels for Bone Repair, Preparation and Evaluation of the Biological Response of Human Trabecular Bone-Derived Cells and Mesenchymal Stem CellsTiago Volkmer0Joana Magalhães1Vania Sousa2Luis A. Santos3Elena F. Burguera4Francisco J. Blanco5Julio San Román6Luis M. Rodríguez-Lorenzo7Biomaterials Laboratory, Federal University of Rio Grande do Sul, 9500 Bento Gonçalves Av., 91509-900 Porto Alegre, RS, BrazilBiomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 28006 Madrid, SpainBiomaterials Laboratory, Federal University of Rio Grande do Sul, 9500 Bento Gonçalves Av., 91509-900 Porto Alegre, RS, BrazilBiomaterials Laboratory, Federal University of Rio Grande do Sul, 9500 Bento Gonçalves Av., 91509-900 Porto Alegre, RS, BrazilBiomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 28006 Madrid, SpainTissue Engineering and Cellular Therapy Group (CBTTC-CHUAC), Servício de Reumatología, Instituto de Investigación Biomédica de A Coruña (INIBIC), Complexo Hospitalario Universitario de A Coruña (CHUAC), Sergas, Universidade da Coruña (UDC), C/As Xubias, 15006 A Coruña, SpainDepartment Polymeric Nanomaterials and Biomaterials, Instituto de Ciencia y Tecnología de Polímeros (ICTP-CSIC), C/Juan de la Cierva, 3, 28006 Madrid, SpainDepartment Polymeric Nanomaterials and Biomaterials, Instituto de Ciencia y Tecnología de Polímeros (ICTP-CSIC), C/Juan de la Cierva, 3, 28006 Madrid, SpainThe aim of this work is to evaluate the potential of cryogels to be used as scaffolds in tissue engineering. Scaffolds based on the α-tricalcium phosphate reinforced PDMAEMA (Poly(dimethyl aminoethyl methacrylate))/PHEMA (poly(hydroxyethyl methacrylate)) system were prepared and human trabecular bone-derived cells (HTBs) and bone marrow derived-mesenchymal stem cells (BM-MSCs) cultured on them. Several features, such as porosity, pore shape, molecular weight between crosslinks and mesh size, are studied. The most suitable PDMAEMA/PHEMA ratio for cell proliferation has been assessed and the viability, adhesion, proliferation and expression of osteoblastic biochemical markers are evaluated. The PDMAEMA/PHEMA ratio influences the scaffolds porosity. Values between 53% ± 5.7% for a greater content in PHEMA and 75% ± 5.5% for a greater content in PDMAEMA have been obtained. The polymer ratio also modifies the pore shape. A greater content in PDMAEMA leads also to bigger network mesh size. Each of the compositions were non-cytotoxic, the seeded cells remained viable for both BM-MSCs and HTBs. Thus, and based on the structural analysis, specimens with a greater content in PDMAEMA seem to provide a better structural environment for their use as scaffolds for tissue engineering. The α-tricalcium phosphate incorporation into the composition seems to favor the expression of the osteogenic phenotype.http://www.mdpi.com/2073-4360/6/10/2510cryogelsα-tricalcium phosphatePDMAEMA (Poly(dimethyl aminoethyl methacrylate))PHEMA (poly(hydroxyethyl methacrylate)mesenchymal stem cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tiago Volkmer Joana Magalhães Vania Sousa Luis A. Santos Elena F. Burguera Francisco J. Blanco Julio San Román Luis M. Rodríguez-Lorenzo |
spellingShingle |
Tiago Volkmer Joana Magalhães Vania Sousa Luis A. Santos Elena F. Burguera Francisco J. Blanco Julio San Román Luis M. Rodríguez-Lorenzo 2-(Dimethylamino)ethyl Methacrylate/(2-Hydroxyethyl) Methacrylate/α-Tricalcium Phosphate Cryogels for Bone Repair, Preparation and Evaluation of the Biological Response of Human Trabecular Bone-Derived Cells and Mesenchymal Stem Cells Polymers cryogels α-tricalcium phosphate PDMAEMA (Poly(dimethyl aminoethyl methacrylate)) PHEMA (poly(hydroxyethyl methacrylate) mesenchymal stem cells |
author_facet |
Tiago Volkmer Joana Magalhães Vania Sousa Luis A. Santos Elena F. Burguera Francisco J. Blanco Julio San Román Luis M. Rodríguez-Lorenzo |
author_sort |
Tiago Volkmer |
title |
2-(Dimethylamino)ethyl Methacrylate/(2-Hydroxyethyl) Methacrylate/α-Tricalcium Phosphate Cryogels for Bone Repair, Preparation and Evaluation of the Biological Response of Human Trabecular Bone-Derived Cells and Mesenchymal Stem Cells |
title_short |
2-(Dimethylamino)ethyl Methacrylate/(2-Hydroxyethyl) Methacrylate/α-Tricalcium Phosphate Cryogels for Bone Repair, Preparation and Evaluation of the Biological Response of Human Trabecular Bone-Derived Cells and Mesenchymal Stem Cells |
title_full |
2-(Dimethylamino)ethyl Methacrylate/(2-Hydroxyethyl) Methacrylate/α-Tricalcium Phosphate Cryogels for Bone Repair, Preparation and Evaluation of the Biological Response of Human Trabecular Bone-Derived Cells and Mesenchymal Stem Cells |
title_fullStr |
2-(Dimethylamino)ethyl Methacrylate/(2-Hydroxyethyl) Methacrylate/α-Tricalcium Phosphate Cryogels for Bone Repair, Preparation and Evaluation of the Biological Response of Human Trabecular Bone-Derived Cells and Mesenchymal Stem Cells |
title_full_unstemmed |
2-(Dimethylamino)ethyl Methacrylate/(2-Hydroxyethyl) Methacrylate/α-Tricalcium Phosphate Cryogels for Bone Repair, Preparation and Evaluation of the Biological Response of Human Trabecular Bone-Derived Cells and Mesenchymal Stem Cells |
title_sort |
2-(dimethylamino)ethyl methacrylate/(2-hydroxyethyl) methacrylate/α-tricalcium phosphate cryogels for bone repair, preparation and evaluation of the biological response of human trabecular bone-derived cells and mesenchymal stem cells |
publisher |
MDPI AG |
series |
Polymers |
issn |
2073-4360 |
publishDate |
2014-09-01 |
description |
The aim of this work is to evaluate the potential of cryogels to be used as scaffolds in tissue engineering. Scaffolds based on the α-tricalcium phosphate reinforced PDMAEMA (Poly(dimethyl aminoethyl methacrylate))/PHEMA (poly(hydroxyethyl methacrylate)) system were prepared and human trabecular bone-derived cells (HTBs) and bone marrow derived-mesenchymal stem cells (BM-MSCs) cultured on them. Several features, such as porosity, pore shape, molecular weight between crosslinks and mesh size, are studied. The most suitable PDMAEMA/PHEMA ratio for cell proliferation has been assessed and the viability, adhesion, proliferation and expression of osteoblastic biochemical markers are evaluated. The PDMAEMA/PHEMA ratio influences the scaffolds porosity. Values between 53% ± 5.7% for a greater content in PHEMA and 75% ± 5.5% for a greater content in PDMAEMA have been obtained. The polymer ratio also modifies the pore shape. A greater content in PDMAEMA leads also to bigger network mesh size. Each of the compositions were non-cytotoxic, the seeded cells remained viable for both BM-MSCs and HTBs. Thus, and based on the structural analysis, specimens with a greater content in PDMAEMA seem to provide a better structural environment for their use as scaffolds for tissue engineering. The α-tricalcium phosphate incorporation into the composition seems to favor the expression of the osteogenic phenotype. |
topic |
cryogels α-tricalcium phosphate PDMAEMA (Poly(dimethyl aminoethyl methacrylate)) PHEMA (poly(hydroxyethyl methacrylate) mesenchymal stem cells |
url |
http://www.mdpi.com/2073-4360/6/10/2510 |
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