Characterization of a Potential Probiotic Lactobacillus brevis RK03 and Efficient Production of γ-Aminobutyric Acid in Batch Fermentation
Lactic acid bacteria were isolated from fish and evaluated for their γ-aminobutyric acid (GABA)-producing abilities. Out of thirty-two isolates, Lactobacillus brevis RK03 showed the highest GABA production ability. The effects of various fermentation parameters including initial glutamic acid level,...
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doaj-85a29a2080484322bfb691df17e854992020-11-24T22:15:51ZengMDPI AGInternational Journal of Molecular Sciences1422-00672018-01-0119114310.3390/ijms19010143ijms19010143Characterization of a Potential Probiotic Lactobacillus brevis RK03 and Efficient Production of γ-Aminobutyric Acid in Batch FermentationChien-Hui Wu0Yi-Huang Hsueh1Jen-Min Kuo2Si-Jia Liu3Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung City 81157, TaiwanGraduate School of Biotechnology and Bioengineering, Yuan Ze University, Taoyuan City 32003, TaiwanDepartment of Seafood Science, National Kaohsiung Marine University, Kaohsiung City 81157, TaiwanDepartment of Seafood Science, National Kaohsiung Marine University, Kaohsiung City 81157, TaiwanLactic acid bacteria were isolated from fish and evaluated for their γ-aminobutyric acid (GABA)-producing abilities. Out of thirty-two isolates, Lactobacillus brevis RK03 showed the highest GABA production ability. The effects of various fermentation parameters including initial glutamic acid level, culture temperature, initial pH, and incubation time on GABA production were investigated via a singleparameter optimization strategy. For industrial large-scale production, a low-cost GABA producing medium (GM) broth was developed for fermentation with L. brevis RK03. We found that an optimized GM broth recipe of 1% glucose; 2.5% yeast extract; 2 ppm each of CaCO3, MnSO4, and Tween 80; and 10 μM pyridoxal phosphate (PLP) resulted in a maximum GABA yield of 62,523 mg/L after 88 h following the addition of 650 mM monosodium glutamate (MSG), for a conversion rate of 93.28%. Our data provide a practical approach for the highly efficient and economic production of GABA. In addition, L. brevis RK03 is highly resistant to gastric acid and bovine bile salt. Thus, the discovery of Lactobacillus strains with the ability to synthesize GABA may offer new opportunities in the design of improved health-promoting functional foods.http://www.mdpi.com/1422-0067/19/1/143gamma aminobutyric acidmonosodium glutamatefermentationLactobacillus brevis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chien-Hui Wu Yi-Huang Hsueh Jen-Min Kuo Si-Jia Liu |
spellingShingle |
Chien-Hui Wu Yi-Huang Hsueh Jen-Min Kuo Si-Jia Liu Characterization of a Potential Probiotic Lactobacillus brevis RK03 and Efficient Production of γ-Aminobutyric Acid in Batch Fermentation International Journal of Molecular Sciences gamma aminobutyric acid monosodium glutamate fermentation Lactobacillus brevis |
author_facet |
Chien-Hui Wu Yi-Huang Hsueh Jen-Min Kuo Si-Jia Liu |
author_sort |
Chien-Hui Wu |
title |
Characterization of a Potential Probiotic Lactobacillus brevis RK03 and Efficient Production of γ-Aminobutyric Acid in Batch Fermentation |
title_short |
Characterization of a Potential Probiotic Lactobacillus brevis RK03 and Efficient Production of γ-Aminobutyric Acid in Batch Fermentation |
title_full |
Characterization of a Potential Probiotic Lactobacillus brevis RK03 and Efficient Production of γ-Aminobutyric Acid in Batch Fermentation |
title_fullStr |
Characterization of a Potential Probiotic Lactobacillus brevis RK03 and Efficient Production of γ-Aminobutyric Acid in Batch Fermentation |
title_full_unstemmed |
Characterization of a Potential Probiotic Lactobacillus brevis RK03 and Efficient Production of γ-Aminobutyric Acid in Batch Fermentation |
title_sort |
characterization of a potential probiotic lactobacillus brevis rk03 and efficient production of γ-aminobutyric acid in batch fermentation |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2018-01-01 |
description |
Lactic acid bacteria were isolated from fish and evaluated for their γ-aminobutyric acid (GABA)-producing abilities. Out of thirty-two isolates, Lactobacillus brevis RK03 showed the highest GABA production ability. The effects of various fermentation parameters including initial glutamic acid level, culture temperature, initial pH, and incubation time on GABA production were investigated via a singleparameter optimization strategy. For industrial large-scale production, a low-cost GABA producing medium (GM) broth was developed for fermentation with L. brevis RK03. We found that an optimized GM broth recipe of 1% glucose; 2.5% yeast extract; 2 ppm each of CaCO3, MnSO4, and Tween 80; and 10 μM pyridoxal phosphate (PLP) resulted in a maximum GABA yield of 62,523 mg/L after 88 h following the addition of 650 mM monosodium glutamate (MSG), for a conversion rate of 93.28%. Our data provide a practical approach for the highly efficient and economic production of GABA. In addition, L. brevis RK03 is highly resistant to gastric acid and bovine bile salt. Thus, the discovery of Lactobacillus strains with the ability to synthesize GABA may offer new opportunities in the design of improved health-promoting functional foods. |
topic |
gamma aminobutyric acid monosodium glutamate fermentation Lactobacillus brevis |
url |
http://www.mdpi.com/1422-0067/19/1/143 |
work_keys_str_mv |
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