| Summary: | Non-typhoidal <i>Salmonella</i> serovars are worldwide spread foodborne pathogens that cause diarrhea in humans and animals. Colonization of gnotobiotic piglet intestine with porcine indigenous mucinolytic <i>Bifidobacterium boum</i> RP36 strain and non-mucinolytic strain RP37 and their interference with <i>Salmonella</i> Typhimurium infection were compared. Bacterial interferences and impact on the host were evaluated by clinical signs of salmonellosis, bacterial translocation, goblet cell count, mRNA expression of mucin 2, villin, claudin-1, claudin-2, and occludin in the ileum and colon, and plasmatic levels of inflammatory cytokines IL-8, TNF-α, and IL-10. Both bifidobacterial strains colonized the intestine comparably. Neither RP36 nor RP37 <i>B. boum</i> strains effectively suppressed signs of salmonellosis. Both <i>B. boum</i> strains suppressed the growth of <i>S</i>. Typhimurium in the ileum and colon. The mucinolytic RP36 strain increased the translocation of <i>S</i>. Typhimurium into the blood, liver, and spleen.
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