Characteristics of Heterotrophic Nitrifying and Aerobic Denitrifying <i>Arthrobacter nicotianae</i> D51 Strain in the Presence of Copper
A heterotrophic nitrification and aerobic denitrification bacterium, strain D51, was identified as <i>Arthrobacter nicotianae</i> based on morphological, phospholipid fatty acids (PLFAs), and 16S rRNA gene sequence analyses. Further tests demonstrated that strain D51 had the capability t...
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doaj-85afdfa71b14467e9f2f76b918152f522020-11-25T00:07:58ZengMDPI AGWater2073-44412019-02-0111343410.3390/w11030434w11030434Characteristics of Heterotrophic Nitrifying and Aerobic Denitrifying <i>Arthrobacter nicotianae</i> D51 Strain in the Presence of CopperXi Cai0Kaili Li1Tengxia He2Yaxin Wang3Xue Zhang4Enyu Xie5Ningning Ding6Zhenlun Li7Chongqing Key Laboratory of Soil Multiscale Interfacial Process, College of Resources and Environment, Southwest University, Chongqing 400716, ChinaSchool of Chemical Engineering, University of Queensland, Brisbane, Queensland 4072, AustraliaChongqing Key Laboratory of Soil Multiscale Interfacial Process, College of Resources and Environment, Southwest University, Chongqing 400716, ChinaChongqing Key Laboratory of Soil Multiscale Interfacial Process, College of Resources and Environment, Southwest University, Chongqing 400716, ChinaChongqing Key Laboratory of Soil Multiscale Interfacial Process, College of Resources and Environment, Southwest University, Chongqing 400716, ChinaChongqing Key Laboratory of Soil Multiscale Interfacial Process, College of Resources and Environment, Southwest University, Chongqing 400716, ChinaChongqing Key Laboratory of Soil Multiscale Interfacial Process, College of Resources and Environment, Southwest University, Chongqing 400716, ChinaChongqing Key Laboratory of Soil Multiscale Interfacial Process, College of Resources and Environment, Southwest University, Chongqing 400716, ChinaA heterotrophic nitrification and aerobic denitrification bacterium, strain D51, was identified as <i>Arthrobacter nicotianae</i> based on morphological, phospholipid fatty acids (PLFAs), and 16S rRNA gene sequence analyses. Further tests demonstrated that strain D51 had the capability to use nitrite, nitrate, or ammonium as the sole nitrogen source in the presence of Cu<sup>2+</sup>. The maximum removal efficiencies of nitrite, nitrate and ammonium were 68.97%, 78.32%, and 98.70%, respectively. Additionally, the maximum growth rate and denitrification capacity of this strain occurred in the presence of 0.05 mg·L<sup>−1</sup> of Cu<sup>2+</sup>.However, the growth and aerobic denitrification capacity were intensively inhibited by Cu<sup>2+</sup> at ≥0.1 mg·L<sup>−1</sup>. Moreover, gas chromatography indicated that a portion of the nitrogen was transformed into N<sub>2</sub>O when the nitrite, nitrate, and ammonium were separately used as the sole nitrogen source. This is the first study of the nitrification and denitrification ability of <i>Arthrobacter nicotianae</i> under aerobic conditions, and the first experiment to investigate the impact of Cu<sup>2+</sup> concentration on the growth and denitrification ability of this bacteria. The results presented herein extend the known varieties of heterotrophic nitrifying⁻aerobic denitrifying bacteria and provide useful information regarding the specific bacteria for nitrogen bioremediation of industrial wastewater containing Cu<sup>2+</sup>.https://www.mdpi.com/2073-4441/11/3/434<i>Arthrobacter nicotianae</i>copperheterotrophic nitrificationaerobic denitrificationnitrogen removal |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xi Cai Kaili Li Tengxia He Yaxin Wang Xue Zhang Enyu Xie Ningning Ding Zhenlun Li |
spellingShingle |
Xi Cai Kaili Li Tengxia He Yaxin Wang Xue Zhang Enyu Xie Ningning Ding Zhenlun Li Characteristics of Heterotrophic Nitrifying and Aerobic Denitrifying <i>Arthrobacter nicotianae</i> D51 Strain in the Presence of Copper Water <i>Arthrobacter nicotianae</i> copper heterotrophic nitrification aerobic denitrification nitrogen removal |
author_facet |
Xi Cai Kaili Li Tengxia He Yaxin Wang Xue Zhang Enyu Xie Ningning Ding Zhenlun Li |
author_sort |
Xi Cai |
title |
Characteristics of Heterotrophic Nitrifying and Aerobic Denitrifying <i>Arthrobacter nicotianae</i> D51 Strain in the Presence of Copper |
title_short |
Characteristics of Heterotrophic Nitrifying and Aerobic Denitrifying <i>Arthrobacter nicotianae</i> D51 Strain in the Presence of Copper |
title_full |
Characteristics of Heterotrophic Nitrifying and Aerobic Denitrifying <i>Arthrobacter nicotianae</i> D51 Strain in the Presence of Copper |
title_fullStr |
Characteristics of Heterotrophic Nitrifying and Aerobic Denitrifying <i>Arthrobacter nicotianae</i> D51 Strain in the Presence of Copper |
title_full_unstemmed |
Characteristics of Heterotrophic Nitrifying and Aerobic Denitrifying <i>Arthrobacter nicotianae</i> D51 Strain in the Presence of Copper |
title_sort |
characteristics of heterotrophic nitrifying and aerobic denitrifying <i>arthrobacter nicotianae</i> d51 strain in the presence of copper |
publisher |
MDPI AG |
series |
Water |
issn |
2073-4441 |
publishDate |
2019-02-01 |
description |
A heterotrophic nitrification and aerobic denitrification bacterium, strain D51, was identified as <i>Arthrobacter nicotianae</i> based on morphological, phospholipid fatty acids (PLFAs), and 16S rRNA gene sequence analyses. Further tests demonstrated that strain D51 had the capability to use nitrite, nitrate, or ammonium as the sole nitrogen source in the presence of Cu<sup>2+</sup>. The maximum removal efficiencies of nitrite, nitrate and ammonium were 68.97%, 78.32%, and 98.70%, respectively. Additionally, the maximum growth rate and denitrification capacity of this strain occurred in the presence of 0.05 mg·L<sup>−1</sup> of Cu<sup>2+</sup>.However, the growth and aerobic denitrification capacity were intensively inhibited by Cu<sup>2+</sup> at ≥0.1 mg·L<sup>−1</sup>. Moreover, gas chromatography indicated that a portion of the nitrogen was transformed into N<sub>2</sub>O when the nitrite, nitrate, and ammonium were separately used as the sole nitrogen source. This is the first study of the nitrification and denitrification ability of <i>Arthrobacter nicotianae</i> under aerobic conditions, and the first experiment to investigate the impact of Cu<sup>2+</sup> concentration on the growth and denitrification ability of this bacteria. The results presented herein extend the known varieties of heterotrophic nitrifying⁻aerobic denitrifying bacteria and provide useful information regarding the specific bacteria for nitrogen bioremediation of industrial wastewater containing Cu<sup>2+</sup>. |
topic |
<i>Arthrobacter nicotianae</i> copper heterotrophic nitrification aerobic denitrification nitrogen removal |
url |
https://www.mdpi.com/2073-4441/11/3/434 |
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