THE FREE RADICAL SCAVENGING AND ANTI-HYPERGLYCEMIC ACTIVITIES OF VARIOUS GAMBIERS AVAILABLE IN INDONESIAN MARKET

• Main Authors: Muhammad Hanafi, Andini Sundowo, Galuh Widiyarti
• Format: Article
• Language: English
• Published: Universitas Indonesia 2011-11-01
• Series: Makara Seri Sains
• Subjects: α-glucosidase; catechin; DPPH; extraction; Uncaria gambier
• Online Access: http://journal.ui.ac.id/science/article/viewFile/1062/975

Gambier (Uncaria gambier) is known to have antioxidant properties, and some studies have attributed it to the presenceof polyphenols such as catechin. The objective of this study is to investigate the potential of various gambiers availablein Indonesian market as a scavenger of reactive free radicals and evaluate its anti-hyperglycemic activity as α-glucosidase inhibitor. Isolation of catechin was done by extraction method with technical grade of ethyl acetate assolvent. Analysis of catechin in the dried gambier extract was carried out with TLC method. The molecular weight andcontent of catechin of dried gambier extract was determined by analyzing its mass spectra and spectrophotometer,respectively. The free radical scavenging activity of catechin of the resultant extracts was measured by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) as stable free radical compound. The anti-hyperglycemic activity of catechin ofethyl acetate extracts was analyzed as α-glucosidase inhibitor. The result showed that various gambiers available in themarket are very active as antioxidant, indicated by IC50 of catechin of ethyl acetate extracts which were 4.6 to 18.2μg/mL for DPPH inhibition. The IC50 for α-glucosidase inhibition is ranged from 40.45 to 52.43 μg/mL, so they can beclassified as anti-diabetic.