Gene Delivery to Nonhuman Primate Preimplantation Embryos Using Recombinant Adeno‐Associated Virus
Abstract Delivery of genome editing tools to mammalian zygotes has revolutionized animal modeling. However, the mechanical delivery method to introduce genes and proteins to zygotes remains a challenge for some animal species that are important in biomedical research. Here, an approach to achieve ge...
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Online Access: | https://doi.org/10.1002/advs.201900440 |
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doaj-85b810096c9048aebd3576b20e45bd6f2020-11-25T01:55:20ZengWileyAdvanced Science2198-38442019-11-01621n/an/a10.1002/advs.201900440Gene Delivery to Nonhuman Primate Preimplantation Embryos Using Recombinant Adeno‐Associated VirusDan Wang0Yuyu Niu1Lingzhi Ren2Yu Kang3Phillip W. L. Tai4Chenyang Si5Craig A. Mendonca6Hong Ma7Guangping Gao8Weizhi Ji9Horae Gene Therapy Center University of Massachusetts Medical School Worcester MA 01605 USAYunnan Key Laboratory of Primate Biomedicine Research Institute of Primate Translational Medicine Kunming University of Science and Technology Kunming 650500 Yunnan ChinaHorae Gene Therapy Center University of Massachusetts Medical School Worcester MA 01605 USAYunnan Key Laboratory of Primate Biomedicine Research Institute of Primate Translational Medicine Kunming University of Science and Technology Kunming 650500 Yunnan ChinaHorae Gene Therapy Center University of Massachusetts Medical School Worcester MA 01605 USAYunnan Key Laboratory of Primate Biomedicine Research Institute of Primate Translational Medicine Kunming University of Science and Technology Kunming 650500 Yunnan ChinaHorae Gene Therapy Center University of Massachusetts Medical School Worcester MA 01605 USAHorae Gene Therapy Center University of Massachusetts Medical School Worcester MA 01605 USAHorae Gene Therapy Center University of Massachusetts Medical School Worcester MA 01605 USAYunnan Key Laboratory of Primate Biomedicine Research Institute of Primate Translational Medicine Kunming University of Science and Technology Kunming 650500 Yunnan ChinaAbstract Delivery of genome editing tools to mammalian zygotes has revolutionized animal modeling. However, the mechanical delivery method to introduce genes and proteins to zygotes remains a challenge for some animal species that are important in biomedical research. Here, an approach to achieve gene delivery and genome editing in nonhuman primate embryos is presented by infecting zygotes with recombinant adeno‐associated viruses (rAAVs). Together with previous reports from the authors of this paper and others, this approach is potentially applicable to a broad range of mammals. In addition to genome editing and animal modeling, this rAAV‐based method can facilitate gene function studies in early‐stage embryos.https://doi.org/10.1002/advs.201900440adeno‐associated virus (AAV)animal modelingCRISPRgenome editing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dan Wang Yuyu Niu Lingzhi Ren Yu Kang Phillip W. L. Tai Chenyang Si Craig A. Mendonca Hong Ma Guangping Gao Weizhi Ji |
spellingShingle |
Dan Wang Yuyu Niu Lingzhi Ren Yu Kang Phillip W. L. Tai Chenyang Si Craig A. Mendonca Hong Ma Guangping Gao Weizhi Ji Gene Delivery to Nonhuman Primate Preimplantation Embryos Using Recombinant Adeno‐Associated Virus Advanced Science adeno‐associated virus (AAV) animal modeling CRISPR genome editing |
author_facet |
Dan Wang Yuyu Niu Lingzhi Ren Yu Kang Phillip W. L. Tai Chenyang Si Craig A. Mendonca Hong Ma Guangping Gao Weizhi Ji |
author_sort |
Dan Wang |
title |
Gene Delivery to Nonhuman Primate Preimplantation Embryos Using Recombinant Adeno‐Associated Virus |
title_short |
Gene Delivery to Nonhuman Primate Preimplantation Embryos Using Recombinant Adeno‐Associated Virus |
title_full |
Gene Delivery to Nonhuman Primate Preimplantation Embryos Using Recombinant Adeno‐Associated Virus |
title_fullStr |
Gene Delivery to Nonhuman Primate Preimplantation Embryos Using Recombinant Adeno‐Associated Virus |
title_full_unstemmed |
Gene Delivery to Nonhuman Primate Preimplantation Embryos Using Recombinant Adeno‐Associated Virus |
title_sort |
gene delivery to nonhuman primate preimplantation embryos using recombinant adeno‐associated virus |
publisher |
Wiley |
series |
Advanced Science |
issn |
2198-3844 |
publishDate |
2019-11-01 |
description |
Abstract Delivery of genome editing tools to mammalian zygotes has revolutionized animal modeling. However, the mechanical delivery method to introduce genes and proteins to zygotes remains a challenge for some animal species that are important in biomedical research. Here, an approach to achieve gene delivery and genome editing in nonhuman primate embryos is presented by infecting zygotes with recombinant adeno‐associated viruses (rAAVs). Together with previous reports from the authors of this paper and others, this approach is potentially applicable to a broad range of mammals. In addition to genome editing and animal modeling, this rAAV‐based method can facilitate gene function studies in early‐stage embryos. |
topic |
adeno‐associated virus (AAV) animal modeling CRISPR genome editing |
url |
https://doi.org/10.1002/advs.201900440 |
work_keys_str_mv |
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