Gene Delivery to Nonhuman Primate Preimplantation Embryos Using Recombinant Adeno‐Associated Virus

Gene Delivery to Nonhuman Primate Preimplantation Embryos Using Recombinant Adeno‐Associated Virus

Abstract Delivery of genome editing tools to mammalian zygotes has revolutionized animal modeling. However, the mechanical delivery method to introduce genes and proteins to zygotes remains a challenge for some animal species that are important in biomedical research. Here, an approach to achieve ge...

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Main Authors: Dan Wang, Yuyu Niu, Lingzhi Ren, Yu Kang, Phillip W. L. Tai, Chenyang Si, Craig A. Mendonca, Hong Ma, Guangping Gao, Weizhi Ji
Format: Article
Language:English
Published: Wiley 2019-11-01
Series:Advanced Science
Subjects:
adeno‐associated virus (AAV)
animal modeling
CRISPR
genome editing
Online Access:https://doi.org/10.1002/advs.201900440
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spelling doaj-85b810096c9048aebd3576b20e45bd6f2020-11-25T01:55:20ZengWileyAdvanced Science2198-38442019-11-01621n/an/a10.1002/advs.201900440Gene Delivery to Nonhuman Primate Preimplantation Embryos Using Recombinant Adeno‐Associated VirusDan Wang0Yuyu Niu1Lingzhi Ren2Yu Kang3Phillip W. L. Tai4Chenyang Si5Craig A. Mendonca6Hong Ma7Guangping Gao8Weizhi Ji9Horae Gene Therapy Center University of Massachusetts Medical School Worcester MA 01605 USAYunnan Key Laboratory of Primate Biomedicine Research Institute of Primate Translational Medicine Kunming University of Science and Technology Kunming 650500 Yunnan ChinaHorae Gene Therapy Center University of Massachusetts Medical School Worcester MA 01605 USAYunnan Key Laboratory of Primate Biomedicine Research Institute of Primate Translational Medicine Kunming University of Science and Technology Kunming 650500 Yunnan ChinaHorae Gene Therapy Center University of Massachusetts Medical School Worcester MA 01605 USAYunnan Key Laboratory of Primate Biomedicine Research Institute of Primate Translational Medicine Kunming University of Science and Technology Kunming 650500 Yunnan ChinaHorae Gene Therapy Center University of Massachusetts Medical School Worcester MA 01605 USAHorae Gene Therapy Center University of Massachusetts Medical School Worcester MA 01605 USAHorae Gene Therapy Center University of Massachusetts Medical School Worcester MA 01605 USAYunnan Key Laboratory of Primate Biomedicine Research Institute of Primate Translational Medicine Kunming University of Science and Technology Kunming 650500 Yunnan ChinaAbstract Delivery of genome editing tools to mammalian zygotes has revolutionized animal modeling. However, the mechanical delivery method to introduce genes and proteins to zygotes remains a challenge for some animal species that are important in biomedical research. Here, an approach to achieve gene delivery and genome editing in nonhuman primate embryos is presented by infecting zygotes with recombinant adeno‐associated viruses (rAAVs). Together with previous reports from the authors of this paper and others, this approach is potentially applicable to a broad range of mammals. In addition to genome editing and animal modeling, this rAAV‐based method can facilitate gene function studies in early‐stage embryos.https://doi.org/10.1002/advs.201900440adeno‐associated virus (AAV)animal modelingCRISPRgenome editing
collection DOAJ
language English
format Article
sources DOAJ
author Dan Wang
Yuyu Niu
Lingzhi Ren
Yu Kang
Phillip W. L. Tai
Chenyang Si
Craig A. Mendonca
Hong Ma
Guangping Gao
Weizhi Ji
spellingShingle Dan Wang
Yuyu Niu
Lingzhi Ren
Yu Kang
Phillip W. L. Tai
Chenyang Si
Craig A. Mendonca
Hong Ma
Guangping Gao
Weizhi Ji
Gene Delivery to Nonhuman Primate Preimplantation Embryos Using Recombinant Adeno‐Associated Virus
Advanced Science
adeno‐associated virus (AAV)
animal modeling
CRISPR
genome editing
author_facet Dan Wang
Yuyu Niu
Lingzhi Ren
Yu Kang
Phillip W. L. Tai
Chenyang Si
Craig A. Mendonca
Hong Ma
Guangping Gao
Weizhi Ji
author_sort Dan Wang
title Gene Delivery to Nonhuman Primate Preimplantation Embryos Using Recombinant Adeno‐Associated Virus
title_short Gene Delivery to Nonhuman Primate Preimplantation Embryos Using Recombinant Adeno‐Associated Virus
title_full Gene Delivery to Nonhuman Primate Preimplantation Embryos Using Recombinant Adeno‐Associated Virus
title_fullStr Gene Delivery to Nonhuman Primate Preimplantation Embryos Using Recombinant Adeno‐Associated Virus
title_full_unstemmed Gene Delivery to Nonhuman Primate Preimplantation Embryos Using Recombinant Adeno‐Associated Virus
title_sort gene delivery to nonhuman primate preimplantation embryos using recombinant adeno‐associated virus
publisher Wiley
series Advanced Science
issn 2198-3844
publishDate 2019-11-01
description Abstract Delivery of genome editing tools to mammalian zygotes has revolutionized animal modeling. However, the mechanical delivery method to introduce genes and proteins to zygotes remains a challenge for some animal species that are important in biomedical research. Here, an approach to achieve gene delivery and genome editing in nonhuman primate embryos is presented by infecting zygotes with recombinant adeno‐associated viruses (rAAVs). Together with previous reports from the authors of this paper and others, this approach is potentially applicable to a broad range of mammals. In addition to genome editing and animal modeling, this rAAV‐based method can facilitate gene function studies in early‐stage embryos.
topic adeno‐associated virus (AAV)
animal modeling
CRISPR
genome editing
url https://doi.org/10.1002/advs.201900440
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