Two Alternative Splicing Variants of AtERF73/HRE1, HRE1α and HRE1β, Have Differential Transactivation Activities in <i>Arabidopsis</i>
AtERF73/HRE1 is an AP2/ERF transcription factor in <i>Arabidopsis</i> and has two distinct alternative splicing variants, HRE1α and HRE1β. In this study, we examined the differences between the molecular functions of HRE1α and HRE1β. We found that HRE1α and HRE1β are both involved in hyp...
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doaj-85b94496fcba47559b6f086dd98c90692020-11-25T03:26:02ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-09-01216984698410.3390/ijms21196984Two Alternative Splicing Variants of AtERF73/HRE1, HRE1α and HRE1β, Have Differential Transactivation Activities in <i>Arabidopsis</i>Hye-Yeon Seok0Jimin Ha1Sun-Young Lee2Hyoungjoon Bae3Yong-Hwan Moon4Institute of Systems Biology, Pusan National University, Busan 46241, KoreaDepartment of Integrated Biological Science, Pusan National University, Busan 46241, KoreaBiological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USADepartment of Integrated Biological Science, Pusan National University, Busan 46241, KoreaInstitute of Systems Biology, Pusan National University, Busan 46241, KoreaAtERF73/HRE1 is an AP2/ERF transcription factor in <i>Arabidopsis</i> and has two distinct alternative splicing variants, HRE1α and HRE1β. In this study, we examined the differences between the molecular functions of HRE1α and HRE1β. We found that HRE1α and HRE1β are both involved in hypoxia response and root development and have transactivation activity. Two conserved motifs in the C-terminal region of HRE1α and HRE1β, EELL and LWSY-like, contributed to their transactivation activity, specifically the four E residues in the EELL motif and the MGLWS amino acid sequence at the end of the LWSY-like motif. The N-terminal region of HRE1β also showed transactivation activity, mediated by the VDDG motif, whereas that of HRE1α did not. The transactivation activity of HRE1β was stronger than that of HRE1α in <i>Arabidopsis</i> protoplasts. Both transcription factors transactivated downstream genes via the GCC box. RNA-sequencing analysis further supported that both HRE1α and HRE1β might regulate gene expression associated with the hypoxia stress response, although they may transactivate different subsets of genes in downstream pathways. Our results, together with previous studies, suggested that HRE1α and HRE1β differentially transactivate downstream genes in hypoxia response and root development in <i>Arabidopsis</i>.https://www.mdpi.com/1422-0067/21/19/6984alternative splicing<i>Arabidopsis</i>HRE1hypoxiaroot developmenttransactivation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hye-Yeon Seok Jimin Ha Sun-Young Lee Hyoungjoon Bae Yong-Hwan Moon |
spellingShingle |
Hye-Yeon Seok Jimin Ha Sun-Young Lee Hyoungjoon Bae Yong-Hwan Moon Two Alternative Splicing Variants of AtERF73/HRE1, HRE1α and HRE1β, Have Differential Transactivation Activities in <i>Arabidopsis</i> International Journal of Molecular Sciences alternative splicing <i>Arabidopsis</i> HRE1 hypoxia root development transactivation |
author_facet |
Hye-Yeon Seok Jimin Ha Sun-Young Lee Hyoungjoon Bae Yong-Hwan Moon |
author_sort |
Hye-Yeon Seok |
title |
Two Alternative Splicing Variants of AtERF73/HRE1, HRE1α and HRE1β, Have Differential Transactivation Activities in <i>Arabidopsis</i> |
title_short |
Two Alternative Splicing Variants of AtERF73/HRE1, HRE1α and HRE1β, Have Differential Transactivation Activities in <i>Arabidopsis</i> |
title_full |
Two Alternative Splicing Variants of AtERF73/HRE1, HRE1α and HRE1β, Have Differential Transactivation Activities in <i>Arabidopsis</i> |
title_fullStr |
Two Alternative Splicing Variants of AtERF73/HRE1, HRE1α and HRE1β, Have Differential Transactivation Activities in <i>Arabidopsis</i> |
title_full_unstemmed |
Two Alternative Splicing Variants of AtERF73/HRE1, HRE1α and HRE1β, Have Differential Transactivation Activities in <i>Arabidopsis</i> |
title_sort |
two alternative splicing variants of aterf73/hre1, hre1α and hre1β, have differential transactivation activities in <i>arabidopsis</i> |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2020-09-01 |
description |
AtERF73/HRE1 is an AP2/ERF transcription factor in <i>Arabidopsis</i> and has two distinct alternative splicing variants, HRE1α and HRE1β. In this study, we examined the differences between the molecular functions of HRE1α and HRE1β. We found that HRE1α and HRE1β are both involved in hypoxia response and root development and have transactivation activity. Two conserved motifs in the C-terminal region of HRE1α and HRE1β, EELL and LWSY-like, contributed to their transactivation activity, specifically the four E residues in the EELL motif and the MGLWS amino acid sequence at the end of the LWSY-like motif. The N-terminal region of HRE1β also showed transactivation activity, mediated by the VDDG motif, whereas that of HRE1α did not. The transactivation activity of HRE1β was stronger than that of HRE1α in <i>Arabidopsis</i> protoplasts. Both transcription factors transactivated downstream genes via the GCC box. RNA-sequencing analysis further supported that both HRE1α and HRE1β might regulate gene expression associated with the hypoxia stress response, although they may transactivate different subsets of genes in downstream pathways. Our results, together with previous studies, suggested that HRE1α and HRE1β differentially transactivate downstream genes in hypoxia response and root development in <i>Arabidopsis</i>. |
topic |
alternative splicing <i>Arabidopsis</i> HRE1 hypoxia root development transactivation |
url |
https://www.mdpi.com/1422-0067/21/19/6984 |
work_keys_str_mv |
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