The association of bone turnover markers with pro- and anti-inflammatory adipokines in patients with gestational diabetes

• Main Authors: Beata Telejko, Katarzyna Kalejta, Mriusz Kuzmicki, Natalia Wawrusiewicz-Kurylonek, Danuta Lipinska, Justyna Pliszka, Juliusz Wilk, Anna Zielinska, Angelika Sobota, Jacek Szamatowicz, Adam Kretowski, Maria Gorska
• Format: Article
• Language: English
• Published: Institute of Rural Health 2015-05-01
• Series: Annals of Agricultural and Environmental Medicine
• Subjects: pregnancy; gestational diabetes; octeocalcin; osteoprotegerin; RANKL; adiponectin
• Online Access: http://www.journalssystem.com/aaem/The-association-of-bone-turnover-markers-with-pro-and-anti-inflammatory-adipokines-in-patients-with-gestational-diabetes,72280,0,2.html
• ISSN: 1232-1966; 1898-2263

Objective The aim of the study was to assess differences in circulating osteocalcin (OC) and osteoprotegerin (OPG), as well as in their expression in subcutaneous adipose tissue (SAT), visceral adipose tissue (VAT) and placental tissue obtained from patients with gestational diabetes mellitus (GDM) and normal glucose tolerance (NGT). Material and Methods Serum levels of OC, OPG and soluble nuclear factor-kB ligand (sRANKL) were measured in 49 women with GDM and 30 subjects with NGT between weeks 24–32 of gestation, and three months after childbirth. OC and OPG mRNA expression was measured in 23 patients with GDM and 23 women with NGT at term, using quantitative real-time RT-PCR. Results The patients with GDM had decreased OC mRNA expression in SAT (p=0.015), lower adiponectin mRNA expression in VAT (p=0.039), and a lower circulating adiponectin level (p=0.04). Multiple regression analysis revealed that serum adiponectin was significantly associated with OC mRNA expression in SAT (b=0.49, p=0.03). Three months postpartum, the OPG/sRANKL ratio was markedly higher in the subjects with prior GDM (p=0.03) and correlated positively with HbA1c (R=0.33; p=0.04), fasting insulin (R=0.35; p=0.03) and HOMA-IR (R=0.34; p=0.04). Conclusions In the patients with GDM decreased OC mRNA expression in SAT might be associated with a reduced stimulatory effect on adiponectin expression in adipose tissue. On the other hand, higher OPG/sRANKL ratio suggests a better protection against bone loss in the subjects with prior GDM.