Determination of Trolox Equivalent Antioxidant Capacity in Berries Using Amperometric Tyrosinase Biosensor Based on Multi-Walled Carbon Nanotubes

In this contribution, Trolox equivalent antioxidant capacity (TEAC) was determined in various berries using carbon paste tyrosinase biosensor with multi-walled carbon nanotubes (MWCNTs), coated with Nafion<sup>®</sup> layer. Electrochemical behaviour of the biosensor and influence of MWC...

Full description

Bibliographic Details
Main Authors:	Arbër Frangu, Amir M. Ashrafi, Milan Sýs, Tahir Arbneshi, Radovan Metelka, Vojtěch Adam, Milan Vlček, Lukáš Richtera
Format:	Article
Language:	English
Published:	MDPI AG 2020-04-01
Series:	Applied Sciences
Subjects:	amperometry ascorbic acid berries biosensor multi-wall carbon nanotubes carbon paste electrode
Online Access:	https://www.mdpi.com/2076-3417/10/7/2497

id	doaj-85c49277098a42518642bc6a6eea97fa
record_format	Article
spelling	doaj-85c49277098a42518642bc6a6eea97fa2020-11-25T02:33:57ZengMDPI AGApplied Sciences2076-34172020-04-01102497249710.3390/app10072497Determination of Trolox Equivalent Antioxidant Capacity in Berries Using Amperometric Tyrosinase Biosensor Based on Multi-Walled Carbon NanotubesArbër Frangu0Amir M. Ashrafi1Milan Sýs2Tahir Arbneshi3Radovan Metelka4Vojtěch Adam5Milan Vlček6Lukáš Richtera7Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Prishtina, Str. Mother Teresa, 10000 Prishtina, KosovoDepartment of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech RepublicDepartment of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentska 573, 53210 Pardubice, Czech RepublicDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, University of Prishtina, Str. Mother Teresa, 10000 Prishtina, KosovoDepartment of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentska 573, 53210 Pardubice, Czech RepublicDepartment of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech RepublicJoint Laboratory of Solid State Chemistry of Institute of Macromolecular Chemistry of the Academy of Sciences of the Czech Republic and the University of Pardubice, 532 10 Pardubice, Czech RepublicDepartment of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech RepublicIn this contribution, Trolox equivalent antioxidant capacity (TEAC) was determined in various berries using carbon paste tyrosinase biosensor with multi-walled carbon nanotubes (MWCNTs), coated with Nafion<sup>®</sup> layer. Electrochemical behaviour of the biosensor and influence of MWCNTs on carbon paste surface were studied with respect to the sensitive amperometric detection of total content of phenolic compounds in berries, expressed as concentration equivalent of Trolox. After optimization of key instrumental and electroanalytical parameters, the biosensor was used for determination of TEAC in blackberries, blueberries, cranberries, raspberries and strawberries by method of multiple standard additions. Electrochemical TEAC assays corresponded well with results obtained by spectrophotometric 1,1-diphenyl-2-picrylhydrazyl radical method, known as DPPH assay. Obtained values were compared with those listed in the National Nutrient Database for additional antioxidant capacity assays as well.https://www.mdpi.com/2076-3417/10/7/2497amperometryascorbic acidberriesbiosensormulti-wall carbon nanotubescarbon paste electrode
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Arbër Frangu Amir M. Ashrafi Milan Sýs Tahir Arbneshi Radovan Metelka Vojtěch Adam Milan Vlček Lukáš Richtera
spellingShingle	Arbër Frangu Amir M. Ashrafi Milan Sýs Tahir Arbneshi Radovan Metelka Vojtěch Adam Milan Vlček Lukáš Richtera Determination of Trolox Equivalent Antioxidant Capacity in Berries Using Amperometric Tyrosinase Biosensor Based on Multi-Walled Carbon Nanotubes Applied Sciences amperometry ascorbic acid berries biosensor multi-wall carbon nanotubes carbon paste electrode
author_facet	Arbër Frangu Amir M. Ashrafi Milan Sýs Tahir Arbneshi Radovan Metelka Vojtěch Adam Milan Vlček Lukáš Richtera
author_sort	Arbër Frangu
title	Determination of Trolox Equivalent Antioxidant Capacity in Berries Using Amperometric Tyrosinase Biosensor Based on Multi-Walled Carbon Nanotubes
title_short	Determination of Trolox Equivalent Antioxidant Capacity in Berries Using Amperometric Tyrosinase Biosensor Based on Multi-Walled Carbon Nanotubes
title_full	Determination of Trolox Equivalent Antioxidant Capacity in Berries Using Amperometric Tyrosinase Biosensor Based on Multi-Walled Carbon Nanotubes
title_fullStr	Determination of Trolox Equivalent Antioxidant Capacity in Berries Using Amperometric Tyrosinase Biosensor Based on Multi-Walled Carbon Nanotubes
title_full_unstemmed	Determination of Trolox Equivalent Antioxidant Capacity in Berries Using Amperometric Tyrosinase Biosensor Based on Multi-Walled Carbon Nanotubes
title_sort	determination of trolox equivalent antioxidant capacity in berries using amperometric tyrosinase biosensor based on multi-walled carbon nanotubes
publisher	MDPI AG
series	Applied Sciences
issn	2076-3417
publishDate	2020-04-01
description	In this contribution, Trolox equivalent antioxidant capacity (TEAC) was determined in various berries using carbon paste tyrosinase biosensor with multi-walled carbon nanotubes (MWCNTs), coated with Nafion<sup>®</sup> layer. Electrochemical behaviour of the biosensor and influence of MWCNTs on carbon paste surface were studied with respect to the sensitive amperometric detection of total content of phenolic compounds in berries, expressed as concentration equivalent of Trolox. After optimization of key instrumental and electroanalytical parameters, the biosensor was used for determination of TEAC in blackberries, blueberries, cranberries, raspberries and strawberries by method of multiple standard additions. Electrochemical TEAC assays corresponded well with results obtained by spectrophotometric 1,1-diphenyl-2-picrylhydrazyl radical method, known as DPPH assay. Obtained values were compared with those listed in the National Nutrient Database for additional antioxidant capacity assays as well.
topic	amperometry ascorbic acid berries biosensor multi-wall carbon nanotubes carbon paste electrode
url	https://www.mdpi.com/2076-3417/10/7/2497
work_keys_str_mv	AT arberfrangu determinationoftroloxequivalentantioxidantcapacityinberriesusingamperometrictyrosinasebiosensorbasedonmultiwalledcarbonnanotubes AT amirmashrafi determinationoftroloxequivalentantioxidantcapacityinberriesusingamperometrictyrosinasebiosensorbasedonmultiwalledcarbonnanotubes AT milansys determinationoftroloxequivalentantioxidantcapacityinberriesusingamperometrictyrosinasebiosensorbasedonmultiwalledcarbonnanotubes AT tahirarbneshi determinationoftroloxequivalentantioxidantcapacityinberriesusingamperometrictyrosinasebiosensorbasedonmultiwalledcarbonnanotubes AT radovanmetelka determinationoftroloxequivalentantioxidantcapacityinberriesusingamperometrictyrosinasebiosensorbasedonmultiwalledcarbonnanotubes AT vojtechadam determinationoftroloxequivalentantioxidantcapacityinberriesusingamperometrictyrosinasebiosensorbasedonmultiwalledcarbonnanotubes AT milanvlcek determinationoftroloxequivalentantioxidantcapacityinberriesusingamperometrictyrosinasebiosensorbasedonmultiwalledcarbonnanotubes AT lukasrichtera determinationoftroloxequivalentantioxidantcapacityinberriesusingamperometrictyrosinasebiosensorbasedonmultiwalledcarbonnanotubes
_version_	1724811295281119232

Determination of Trolox Equivalent Antioxidant Capacity in Berries Using Amperometric Tyrosinase Biosensor Based on Multi-Walled Carbon Nanotubes

Similar Items