New insights into the bacterial fitness-associated mechanisms revealed by the characterization of large plasmids of an avian pathogenic E. coli.
Extra-intestinal pathogenic E. coli (ExPEC), including avian pathogenic E. coli (APEC), pose a considerable threat to both human and animal health, with illness causing substantial economic loss. APEC strain χ7122 (O78∶K80∶H9), containing three large plasmids [pChi7122-1 (IncFIB/FIIA-FIC), pChi7122-...
Main Authors: | , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2012-01-01
|
Series: | PLoS ONE |
Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22238616/?tool=EBI |
id |
doaj-85ca0a5eeb33458ab5e993ecc1b9c170 |
---|---|
record_format |
Article |
spelling |
doaj-85ca0a5eeb33458ab5e993ecc1b9c1702021-03-04T12:37:40ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0171e2948110.1371/journal.pone.0029481New insights into the bacterial fitness-associated mechanisms revealed by the characterization of large plasmids of an avian pathogenic E. coli.Melha MellataJacob T MadduxTimothy NamNicholas ThomsonHeidi HauserMark P StevensSuman MukhopadhyayShameema SarkerAurélie CrabbéCheryl A NickersonJavier SantanderRoy CurtissExtra-intestinal pathogenic E. coli (ExPEC), including avian pathogenic E. coli (APEC), pose a considerable threat to both human and animal health, with illness causing substantial economic loss. APEC strain χ7122 (O78∶K80∶H9), containing three large plasmids [pChi7122-1 (IncFIB/FIIA-FIC), pChi7122-2 (IncFII), and pChi7122-3 (IncI(2))]; and a small plasmid pChi7122-4 (ColE2-like), has been used for many years as a model strain to study the molecular mechanisms of ExPEC pathogenicity and zoonotic potential. We previously sequenced and characterized the plasmid pChi7122-1 and determined its importance in systemic APEC infection; however the roles of the other pChi7122 plasmids were still ambiguous. Herein we present the sequence of the remaining pChi7122 plasmids, confirming that pChi7122-2 and pChi7122-3 encode an ABC iron transport system (eitABCD) and a putative type IV fimbriae respectively, whereas pChi7122-4 is a cryptic plasmid. New features were also identified, including a gene cluster on pChi7122-2 that is not present in other E. coli strains but is found in Salmonella serovars and is predicted to encode the sugars catabolic pathways. In vitro evaluation of the APEC χ7122 derivative strains with the three large plasmids, either individually or in combinations, provided new insights into the role of plasmids in biofilm formation, bile and acid tolerance, and the interaction of E. coli strains with 3-D cultures of intestinal epithelial cells. In this study, we show that the nature and combinations of plasmids, as well as the background of the host strains, have an effect on these phenomena. Our data reveal new insights into the role of extra-chromosomal sequences in fitness and diversity of ExPEC in their phenotypes.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22238616/?tool=EBI |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Melha Mellata Jacob T Maddux Timothy Nam Nicholas Thomson Heidi Hauser Mark P Stevens Suman Mukhopadhyay Shameema Sarker Aurélie Crabbé Cheryl A Nickerson Javier Santander Roy Curtiss |
spellingShingle |
Melha Mellata Jacob T Maddux Timothy Nam Nicholas Thomson Heidi Hauser Mark P Stevens Suman Mukhopadhyay Shameema Sarker Aurélie Crabbé Cheryl A Nickerson Javier Santander Roy Curtiss New insights into the bacterial fitness-associated mechanisms revealed by the characterization of large plasmids of an avian pathogenic E. coli. PLoS ONE |
author_facet |
Melha Mellata Jacob T Maddux Timothy Nam Nicholas Thomson Heidi Hauser Mark P Stevens Suman Mukhopadhyay Shameema Sarker Aurélie Crabbé Cheryl A Nickerson Javier Santander Roy Curtiss |
author_sort |
Melha Mellata |
title |
New insights into the bacterial fitness-associated mechanisms revealed by the characterization of large plasmids of an avian pathogenic E. coli. |
title_short |
New insights into the bacterial fitness-associated mechanisms revealed by the characterization of large plasmids of an avian pathogenic E. coli. |
title_full |
New insights into the bacterial fitness-associated mechanisms revealed by the characterization of large plasmids of an avian pathogenic E. coli. |
title_fullStr |
New insights into the bacterial fitness-associated mechanisms revealed by the characterization of large plasmids of an avian pathogenic E. coli. |
title_full_unstemmed |
New insights into the bacterial fitness-associated mechanisms revealed by the characterization of large plasmids of an avian pathogenic E. coli. |
title_sort |
new insights into the bacterial fitness-associated mechanisms revealed by the characterization of large plasmids of an avian pathogenic e. coli. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
Extra-intestinal pathogenic E. coli (ExPEC), including avian pathogenic E. coli (APEC), pose a considerable threat to both human and animal health, with illness causing substantial economic loss. APEC strain χ7122 (O78∶K80∶H9), containing three large plasmids [pChi7122-1 (IncFIB/FIIA-FIC), pChi7122-2 (IncFII), and pChi7122-3 (IncI(2))]; and a small plasmid pChi7122-4 (ColE2-like), has been used for many years as a model strain to study the molecular mechanisms of ExPEC pathogenicity and zoonotic potential. We previously sequenced and characterized the plasmid pChi7122-1 and determined its importance in systemic APEC infection; however the roles of the other pChi7122 plasmids were still ambiguous. Herein we present the sequence of the remaining pChi7122 plasmids, confirming that pChi7122-2 and pChi7122-3 encode an ABC iron transport system (eitABCD) and a putative type IV fimbriae respectively, whereas pChi7122-4 is a cryptic plasmid. New features were also identified, including a gene cluster on pChi7122-2 that is not present in other E. coli strains but is found in Salmonella serovars and is predicted to encode the sugars catabolic pathways. In vitro evaluation of the APEC χ7122 derivative strains with the three large plasmids, either individually or in combinations, provided new insights into the role of plasmids in biofilm formation, bile and acid tolerance, and the interaction of E. coli strains with 3-D cultures of intestinal epithelial cells. In this study, we show that the nature and combinations of plasmids, as well as the background of the host strains, have an effect on these phenomena. Our data reveal new insights into the role of extra-chromosomal sequences in fitness and diversity of ExPEC in their phenotypes. |
url |
https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22238616/?tool=EBI |
work_keys_str_mv |
AT melhamellata newinsightsintothebacterialfitnessassociatedmechanismsrevealedbythecharacterizationoflargeplasmidsofanavianpathogenicecoli AT jacobtmaddux newinsightsintothebacterialfitnessassociatedmechanismsrevealedbythecharacterizationoflargeplasmidsofanavianpathogenicecoli AT timothynam newinsightsintothebacterialfitnessassociatedmechanismsrevealedbythecharacterizationoflargeplasmidsofanavianpathogenicecoli AT nicholasthomson newinsightsintothebacterialfitnessassociatedmechanismsrevealedbythecharacterizationoflargeplasmidsofanavianpathogenicecoli AT heidihauser newinsightsintothebacterialfitnessassociatedmechanismsrevealedbythecharacterizationoflargeplasmidsofanavianpathogenicecoli AT markpstevens newinsightsintothebacterialfitnessassociatedmechanismsrevealedbythecharacterizationoflargeplasmidsofanavianpathogenicecoli AT sumanmukhopadhyay newinsightsintothebacterialfitnessassociatedmechanismsrevealedbythecharacterizationoflargeplasmidsofanavianpathogenicecoli AT shameemasarker newinsightsintothebacterialfitnessassociatedmechanismsrevealedbythecharacterizationoflargeplasmidsofanavianpathogenicecoli AT aureliecrabbe newinsightsintothebacterialfitnessassociatedmechanismsrevealedbythecharacterizationoflargeplasmidsofanavianpathogenicecoli AT cherylanickerson newinsightsintothebacterialfitnessassociatedmechanismsrevealedbythecharacterizationoflargeplasmidsofanavianpathogenicecoli AT javiersantander newinsightsintothebacterialfitnessassociatedmechanismsrevealedbythecharacterizationoflargeplasmidsofanavianpathogenicecoli AT roycurtiss newinsightsintothebacterialfitnessassociatedmechanismsrevealedbythecharacterizationoflargeplasmidsofanavianpathogenicecoli |
_version_ |
1714802155470192640 |