Modification of Luffa Sponge for Enrichment of Phosphopeptides
The enrichment technique is crucial to the comprehensive analysis of protein phosphorylation. In this work, a facile, green and efficient synthetic method was set up for quaternization of luffa sponge. The resultant luffa sponge showed strong anion-exchange characteristics and a high adsorption abil...
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doaj-85d9ccbb76614bd993ee2773c17a61b02020-11-25T02:06:56ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-12-0121110110.3390/ijms21010101ijms21010101Modification of Luffa Sponge for Enrichment of PhosphopeptidesLili Dai0Zhe Sun1Ping Zhou2Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, ChinaKey Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, ChinaKey Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, ChinaThe enrichment technique is crucial to the comprehensive analysis of protein phosphorylation. In this work, a facile, green and efficient synthetic method was set up for quaternization of luffa sponge. The resultant luffa sponge showed strong anion-exchange characteristics and a high adsorption ability for phosphate ions. Along with the unique physical properties, e.g., tenacity and porous texture, quaternized luffa sponge was demonstrated to be a well-suited solid-phase extraction (SPE) material. The quaternized luffa sponge-based SPE method was simple, cost-effective and convenient in operation, and was successfully applied to the capture of phosphopeptides from protein digests. The enrichment approach exhibited exceptionally high selectivity, sensitivity and strong anti-interference ability. Four phosphopeptides were still detected by using the digest mixture of β-casein and bovine serum albumin with a molar ratio of 1:100. 21 phosphopeptides were identified from the tryptic digest of non-fat milk.https://www.mdpi.com/1422-0067/21/1/101luffa spongephosphopeptidemass spectrometrymatrix-assisted laser desorption ionizationsolid-phase extraction |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lili Dai Zhe Sun Ping Zhou |
spellingShingle |
Lili Dai Zhe Sun Ping Zhou Modification of Luffa Sponge for Enrichment of Phosphopeptides International Journal of Molecular Sciences luffa sponge phosphopeptide mass spectrometry matrix-assisted laser desorption ionization solid-phase extraction |
author_facet |
Lili Dai Zhe Sun Ping Zhou |
author_sort |
Lili Dai |
title |
Modification of Luffa Sponge for Enrichment of Phosphopeptides |
title_short |
Modification of Luffa Sponge for Enrichment of Phosphopeptides |
title_full |
Modification of Luffa Sponge for Enrichment of Phosphopeptides |
title_fullStr |
Modification of Luffa Sponge for Enrichment of Phosphopeptides |
title_full_unstemmed |
Modification of Luffa Sponge for Enrichment of Phosphopeptides |
title_sort |
modification of luffa sponge for enrichment of phosphopeptides |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2019-12-01 |
description |
The enrichment technique is crucial to the comprehensive analysis of protein phosphorylation. In this work, a facile, green and efficient synthetic method was set up for quaternization of luffa sponge. The resultant luffa sponge showed strong anion-exchange characteristics and a high adsorption ability for phosphate ions. Along with the unique physical properties, e.g., tenacity and porous texture, quaternized luffa sponge was demonstrated to be a well-suited solid-phase extraction (SPE) material. The quaternized luffa sponge-based SPE method was simple, cost-effective and convenient in operation, and was successfully applied to the capture of phosphopeptides from protein digests. The enrichment approach exhibited exceptionally high selectivity, sensitivity and strong anti-interference ability. Four phosphopeptides were still detected by using the digest mixture of β-casein and bovine serum albumin with a molar ratio of 1:100. 21 phosphopeptides were identified from the tryptic digest of non-fat milk. |
topic |
luffa sponge phosphopeptide mass spectrometry matrix-assisted laser desorption ionization solid-phase extraction |
url |
https://www.mdpi.com/1422-0067/21/1/101 |
work_keys_str_mv |
AT lilidai modificationofluffaspongeforenrichmentofphosphopeptides AT zhesun modificationofluffaspongeforenrichmentofphosphopeptides AT pingzhou modificationofluffaspongeforenrichmentofphosphopeptides |
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