Blueberry-Derived Exosome-Like Nanoparticles Counters the Response to TNF-α-Induced Change on Gene Expression in EA.hy926 Cells
Exosome-like nanoparticles (ELNs) are attracting interest as important vehicles of intercellular communication, both in prokaryotes and eukaryotes. Recently, dietary nanoparticles similar to mammalian exosomes have attracted attention for these features. In particular they appear to be relevant in t...
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doaj-85f14ec46cde4ac59fe9ab8b25cdd38c2020-11-25T02:01:35ZengMDPI AGBiomolecules2218-273X2020-05-011074274210.3390/biom10050742Blueberry-Derived Exosome-Like Nanoparticles Counters the Response to TNF-α-Induced Change on Gene Expression in EA.hy926 CellsMariangela De Robertis0Angelo Sarra1Valentina D’Oria2Francesco Mura3Federico Bordi4Paolo Postorino5Deborah Fratantonio6Institute of Biomembranes, Bioenergetics and Molecular Biotechnologies, Consiglio Nazionale delle Ricerche (CNR), 70126 Bari, ItalyDepartment of Science, University of Roma Tre, 00145 Rome, ItalyBambino Gesù Children’s Hospital IRCCS, Research Laboratories, 00146 Rome, ItalyCenter for Nanotechnology for Engineering (CNIS), Sapienza University of Rome, 00185 Rome, ItalyCNR-ISC UOS Sapienza, Sapienza University of Rome, 00185 Rome, ItalyDepartment of Physics, Sapienza University of Rome, 00185 Rome, ItalyDepartment of Biosciences, Biotechnology and Biopharmaceutics, University of Bari Aldo Moro, 70125 Bari, ItalyExosome-like nanoparticles (ELNs) are attracting interest as important vehicles of intercellular communication, both in prokaryotes and eukaryotes. Recently, dietary nanoparticles similar to mammalian exosomes have attracted attention for these features. In particular they appear to be relevant in the modulation of several cellular processes as well as candidate carriers of bioactive molecules (proteins, lipids, and nucleic acids, including miRNAs) with therapeutic value. Herein, we investigated the cellular uptake of blueberry-derived ELNs (B-ELNs) by a human stabilized endothelial cell line (EA.hy926) and the ability of B-ELNs to modulate the expression of inflammatory genes as the response of tumor necrosis factor-α (TNF-α). Our results indicate that 1) EA.hy926 cells internalize B-ELNs in a dose-dependent manner; 2) pretreatment with B-ELNs counters TNF-α-induced reactive oxygen species (ROS) generation and loss of cell viability and modulates the differential expression of 29 genes (fold change > 1.5) induced by TNF-α compared to control; 3) pathway analysis reveals their involvement in a total of 340 canonical pathways, 121 KEGG pathways, and 121 GO Biological processes; and 4) the intersection between differentially expressed (DE) genes and miRNAs contained in B-ELNs unveils a set of candidate target genes, such as prostaglandin I2 synthase (PTGIS), mitogen-activated protein kinase 14 (MAPK14), and phosphodiesterase 7A (PDE7A), for ELNs-contained cargo. In conclusion, our study indicates that B-ELNs can be considered candidate therapeutic carriers of bioactive compounds potentially able to protect vascular system against various stressors.https://www.mdpi.com/2218-273X/10/5/742blueberrycross-kingdomexosome-like nanoparticlesgene expressioninflammationoxidative stress |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mariangela De Robertis Angelo Sarra Valentina D’Oria Francesco Mura Federico Bordi Paolo Postorino Deborah Fratantonio |
spellingShingle |
Mariangela De Robertis Angelo Sarra Valentina D’Oria Francesco Mura Federico Bordi Paolo Postorino Deborah Fratantonio Blueberry-Derived Exosome-Like Nanoparticles Counters the Response to TNF-α-Induced Change on Gene Expression in EA.hy926 Cells Biomolecules blueberry cross-kingdom exosome-like nanoparticles gene expression inflammation oxidative stress |
author_facet |
Mariangela De Robertis Angelo Sarra Valentina D’Oria Francesco Mura Federico Bordi Paolo Postorino Deborah Fratantonio |
author_sort |
Mariangela De Robertis |
title |
Blueberry-Derived Exosome-Like Nanoparticles Counters the Response to TNF-α-Induced Change on Gene Expression in EA.hy926 Cells |
title_short |
Blueberry-Derived Exosome-Like Nanoparticles Counters the Response to TNF-α-Induced Change on Gene Expression in EA.hy926 Cells |
title_full |
Blueberry-Derived Exosome-Like Nanoparticles Counters the Response to TNF-α-Induced Change on Gene Expression in EA.hy926 Cells |
title_fullStr |
Blueberry-Derived Exosome-Like Nanoparticles Counters the Response to TNF-α-Induced Change on Gene Expression in EA.hy926 Cells |
title_full_unstemmed |
Blueberry-Derived Exosome-Like Nanoparticles Counters the Response to TNF-α-Induced Change on Gene Expression in EA.hy926 Cells |
title_sort |
blueberry-derived exosome-like nanoparticles counters the response to tnf-α-induced change on gene expression in ea.hy926 cells |
publisher |
MDPI AG |
series |
Biomolecules |
issn |
2218-273X |
publishDate |
2020-05-01 |
description |
Exosome-like nanoparticles (ELNs) are attracting interest as important vehicles of intercellular communication, both in prokaryotes and eukaryotes. Recently, dietary nanoparticles similar to mammalian exosomes have attracted attention for these features. In particular they appear to be relevant in the modulation of several cellular processes as well as candidate carriers of bioactive molecules (proteins, lipids, and nucleic acids, including miRNAs) with therapeutic value. Herein, we investigated the cellular uptake of blueberry-derived ELNs (B-ELNs) by a human stabilized endothelial cell line (EA.hy926) and the ability of B-ELNs to modulate the expression of inflammatory genes as the response of tumor necrosis factor-α (TNF-α). Our results indicate that 1) EA.hy926 cells internalize B-ELNs in a dose-dependent manner; 2) pretreatment with B-ELNs counters TNF-α-induced reactive oxygen species (ROS) generation and loss of cell viability and modulates the differential expression of 29 genes (fold change > 1.5) induced by TNF-α compared to control; 3) pathway analysis reveals their involvement in a total of 340 canonical pathways, 121 KEGG pathways, and 121 GO Biological processes; and 4) the intersection between differentially expressed (DE) genes and miRNAs contained in B-ELNs unveils a set of candidate target genes, such as prostaglandin I2 synthase (PTGIS), mitogen-activated protein kinase 14 (MAPK14), and phosphodiesterase 7A (PDE7A), for ELNs-contained cargo. In conclusion, our study indicates that B-ELNs can be considered candidate therapeutic carriers of bioactive compounds potentially able to protect vascular system against various stressors. |
topic |
blueberry cross-kingdom exosome-like nanoparticles gene expression inflammation oxidative stress |
url |
https://www.mdpi.com/2218-273X/10/5/742 |
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