Diagnostic DNA-Flow- vs. -Image-Cytometry in Effusion Cytology
Aims: To determine the sensitivity and specificity of flow‐ and image‐cytometry for the detection of DNA‐aneuploidy as a marker for malignant cells in effusions. Methods: 200 effusions (80 tumor cell‐positive, 74 negative and 46 cytologically equivocal) were stained with DAPI‐SR for DNA‐flow‐ and wi...
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Hindawi Limited
2002-01-01
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Series: | Analytical Cellular Pathology |
Online Access: | http://dx.doi.org/10.1155/2002/840210 |
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doaj-860aacc0af5a490cb07c550f2f5b630f2020-11-24T22:36:27ZengHindawi LimitedAnalytical Cellular Pathology0921-89121878-36512002-01-0124151510.1155/2002/840210Diagnostic DNA-Flow- vs. -Image-Cytometry in Effusion CytologyHelma Motherby0Natalia Pomjanski1Mary Kube2Alexandra Boros3Thomas Heiden4Bernhard Tribukait5Alfred Böcking6Institute of Cytopathology, Heinrich‐Heine‐University, Moorenstr. 5, D‐40225 Düsseldorf, GermanyInstitute of Cytopathology, Heinrich‐Heine‐University, Moorenstr. 5, D‐40225 Düsseldorf, GermanyInstitute of Cytopathology, Heinrich‐Heine‐University, Moorenstr. 5, D‐40225 Düsseldorf, GermanyInstitute of Cytopathology, Heinrich‐Heine‐University, Moorenstr. 5, D‐40225 Düsseldorf, GermanyDepartment of Medical Radiobiology, Karolinska Institute, S‐10401 Stockholm, SwedenDepartment of Medical Radiobiology, Karolinska Institute, S‐10401 Stockholm, SwedenInstitute of Cytopathology, Heinrich‐Heine‐University, Moorenstr. 5, D‐40225 Düsseldorf, GermanyAims: To determine the sensitivity and specificity of flow‐ and image‐cytometry for the detection of DNA‐aneuploidy as a marker for malignant cells in effusions. Methods: 200 effusions (80 tumor cell‐positive, 74 negative and 46 cytologically equivocal) were stained with DAPI‐SR for DNA‐flow‐ and with Feulgen‐Pararosaniline for ‐image‐cytometry. They were measured using a PAS‐flow‐cytometer and an AutoCyte‐QUIC‐DNA‐workstation according to the ESACP consensus reports for DNA‐flow‐ and ‐image‐cytometry, respectively [7,23,29,49]. Results: Sensitivity of DNA‐aneuploidy for the identification of malignant cells was 32.1% for DNA‐flow‐ and 75.0% for ‐image‐cytometry, specificity of ‐euploidy in benign cells was 100.0% for both methods. Positive predictive value of DNA‐aneuploidy for the identification of malignant cells was 100.0% for both techniques, negative predictive value of DNA‐euploidy was 48.6% for DNA‐flow‐ and 72.0% for ‐image‐cytometry. Conclusions: Searching for DNA‐aneuploidy as a diagnostic marker for neoplastic cells in serous effusions image‐cytometry revealed superior sensitivity as compared with monoparametric flow cytometry.http://dx.doi.org/10.1155/2002/840210 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Helma Motherby Natalia Pomjanski Mary Kube Alexandra Boros Thomas Heiden Bernhard Tribukait Alfred Böcking |
spellingShingle |
Helma Motherby Natalia Pomjanski Mary Kube Alexandra Boros Thomas Heiden Bernhard Tribukait Alfred Böcking Diagnostic DNA-Flow- vs. -Image-Cytometry in Effusion Cytology Analytical Cellular Pathology |
author_facet |
Helma Motherby Natalia Pomjanski Mary Kube Alexandra Boros Thomas Heiden Bernhard Tribukait Alfred Böcking |
author_sort |
Helma Motherby |
title |
Diagnostic DNA-Flow- vs. -Image-Cytometry in Effusion Cytology |
title_short |
Diagnostic DNA-Flow- vs. -Image-Cytometry in Effusion Cytology |
title_full |
Diagnostic DNA-Flow- vs. -Image-Cytometry in Effusion Cytology |
title_fullStr |
Diagnostic DNA-Flow- vs. -Image-Cytometry in Effusion Cytology |
title_full_unstemmed |
Diagnostic DNA-Flow- vs. -Image-Cytometry in Effusion Cytology |
title_sort |
diagnostic dna-flow- vs. -image-cytometry in effusion cytology |
publisher |
Hindawi Limited |
series |
Analytical Cellular Pathology |
issn |
0921-8912 1878-3651 |
publishDate |
2002-01-01 |
description |
Aims: To determine the sensitivity and specificity of flow‐ and image‐cytometry for the detection of DNA‐aneuploidy as a marker for malignant cells in effusions. Methods: 200 effusions (80 tumor cell‐positive, 74 negative and 46 cytologically equivocal) were stained with DAPI‐SR for DNA‐flow‐ and with Feulgen‐Pararosaniline for ‐image‐cytometry. They were measured using a PAS‐flow‐cytometer and an AutoCyte‐QUIC‐DNA‐workstation according to the ESACP consensus reports for DNA‐flow‐ and ‐image‐cytometry, respectively [7,23,29,49]. Results: Sensitivity of DNA‐aneuploidy for the identification of malignant cells was 32.1% for DNA‐flow‐ and 75.0% for ‐image‐cytometry, specificity of ‐euploidy in benign cells was 100.0% for both methods. Positive predictive value of DNA‐aneuploidy for the identification of malignant cells was 100.0% for both techniques, negative predictive value of DNA‐euploidy was 48.6% for DNA‐flow‐ and 72.0% for ‐image‐cytometry. Conclusions: Searching for DNA‐aneuploidy as a diagnostic marker for neoplastic cells in serous effusions image‐cytometry revealed superior sensitivity as compared with monoparametric flow cytometry. |
url |
http://dx.doi.org/10.1155/2002/840210 |
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