A Novel Reassortant Avian H7N6 Influenza Virus Is Transmissible in Guinea Pigs via Respiratory Droplets
Since 2013, H7N9 and H5N6 avian influenza viruses (AIVs) have caused sporadic human infections and deaths and continued to circulate in the poultry industry. Since 2014, H7N6 viruses which might be reassortants of H7N9 and H5N6 viruses, have been isolated in China. However, the biological properties...
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doaj-863f34d3ef7c44a2add5707ee9f922432020-11-24T22:24:07ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-01-011010.3389/fmicb.2019.00018432268A Novel Reassortant Avian H7N6 Influenza Virus Is Transmissible in Guinea Pigs via Respiratory DropletsZongzheng Zhao0Zongzheng Zhao1Lina Liu2Zhendong Guo3Chunmao Zhang4Zhongyi Wang5Guoyuan Wen6Wenting Zhang7Yu Shang8Tengfei Zhang9Zuwu Jiao10Ligong Chen11Cheng Zhang12Huan Cui13Meilin Jin14Chengyu Wang15Qingping Luo16Huabin Shao17Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, ChinaInstitute of Military Veterinary, Academy of Military Medical Sciences, Changchun, ChinaInstitute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, ChinaInstitute of Military Veterinary, Academy of Military Medical Sciences, Changchun, ChinaInstitute of Military Veterinary, Academy of Military Medical Sciences, Changchun, ChinaInstitute of Military Veterinary, Academy of Military Medical Sciences, Changchun, ChinaInstitute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, ChinaInstitute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, ChinaInstitute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, ChinaInstitute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, ChinaInstitute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, ChinaCollege of Veterinary Medicine, Hebei Agricultural University, Baoding, ChinaCollege of Veterinary Medicine, Hebei Agricultural University, Baoding, ChinaCollege of Veterinary Medicine, Hebei Agricultural University, Baoding, ChinaCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaInstitute of Military Veterinary, Academy of Military Medical Sciences, Changchun, ChinaInstitute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, ChinaInstitute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, ChinaSince 2013, H7N9 and H5N6 avian influenza viruses (AIVs) have caused sporadic human infections and deaths and continued to circulate in the poultry industry. Since 2014, H7N6 viruses which might be reassortants of H7N9 and H5N6 viruses, have been isolated in China. However, the biological properties of H7N6 viruses are unknown. Here, we characterize the receptor binding preference, pathogenicity and transmissibility of a H7N6 virus A/chicken/Hubei/00095/2017(H7N6) (abbreviated HB95), and a closely related H7N9 virus, A/chicken/Hubei/00093/2017(H7N9) (abbreviated HB93), which were isolated from poultry in Hubei Province, China, in 2017. Phylogenetic analyses demonstrated that the hemagglutinin (HA) gene of HB95 is closely related to those of HB93 and human-origin H7N9 viruses, and that the neuraminidase (NA) gene of HB95 shared the highest nucleotide similarity with those of H5N6 viruses. HB95 and HB93 had binding affinity for human-like α2, 6-linked sialic acid receptors and were virulent in mice without prior adaptation. In addition, in guinea pig model, HB93 was transmissible by direct contact, but HB95 was transmissible via respiratory droplets. These results revealed the potential threat to public health posed by H7N6 influenza viruses and emphasized the need for continued surveillance of the circulation of this subtype in poultry.https://www.frontiersin.org/article/10.3389/fmicb.2019.00018/fullavian H7N6 influenza A virusreassortmentreceptor bindingpathogenicitytransmissibility |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zongzheng Zhao Zongzheng Zhao Lina Liu Zhendong Guo Chunmao Zhang Zhongyi Wang Guoyuan Wen Wenting Zhang Yu Shang Tengfei Zhang Zuwu Jiao Ligong Chen Cheng Zhang Huan Cui Meilin Jin Chengyu Wang Qingping Luo Huabin Shao |
spellingShingle |
Zongzheng Zhao Zongzheng Zhao Lina Liu Zhendong Guo Chunmao Zhang Zhongyi Wang Guoyuan Wen Wenting Zhang Yu Shang Tengfei Zhang Zuwu Jiao Ligong Chen Cheng Zhang Huan Cui Meilin Jin Chengyu Wang Qingping Luo Huabin Shao A Novel Reassortant Avian H7N6 Influenza Virus Is Transmissible in Guinea Pigs via Respiratory Droplets Frontiers in Microbiology avian H7N6 influenza A virus reassortment receptor binding pathogenicity transmissibility |
author_facet |
Zongzheng Zhao Zongzheng Zhao Lina Liu Zhendong Guo Chunmao Zhang Zhongyi Wang Guoyuan Wen Wenting Zhang Yu Shang Tengfei Zhang Zuwu Jiao Ligong Chen Cheng Zhang Huan Cui Meilin Jin Chengyu Wang Qingping Luo Huabin Shao |
author_sort |
Zongzheng Zhao |
title |
A Novel Reassortant Avian H7N6 Influenza Virus Is Transmissible in Guinea Pigs via Respiratory Droplets |
title_short |
A Novel Reassortant Avian H7N6 Influenza Virus Is Transmissible in Guinea Pigs via Respiratory Droplets |
title_full |
A Novel Reassortant Avian H7N6 Influenza Virus Is Transmissible in Guinea Pigs via Respiratory Droplets |
title_fullStr |
A Novel Reassortant Avian H7N6 Influenza Virus Is Transmissible in Guinea Pigs via Respiratory Droplets |
title_full_unstemmed |
A Novel Reassortant Avian H7N6 Influenza Virus Is Transmissible in Guinea Pigs via Respiratory Droplets |
title_sort |
novel reassortant avian h7n6 influenza virus is transmissible in guinea pigs via respiratory droplets |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2019-01-01 |
description |
Since 2013, H7N9 and H5N6 avian influenza viruses (AIVs) have caused sporadic human infections and deaths and continued to circulate in the poultry industry. Since 2014, H7N6 viruses which might be reassortants of H7N9 and H5N6 viruses, have been isolated in China. However, the biological properties of H7N6 viruses are unknown. Here, we characterize the receptor binding preference, pathogenicity and transmissibility of a H7N6 virus A/chicken/Hubei/00095/2017(H7N6) (abbreviated HB95), and a closely related H7N9 virus, A/chicken/Hubei/00093/2017(H7N9) (abbreviated HB93), which were isolated from poultry in Hubei Province, China, in 2017. Phylogenetic analyses demonstrated that the hemagglutinin (HA) gene of HB95 is closely related to those of HB93 and human-origin H7N9 viruses, and that the neuraminidase (NA) gene of HB95 shared the highest nucleotide similarity with those of H5N6 viruses. HB95 and HB93 had binding affinity for human-like α2, 6-linked sialic acid receptors and were virulent in mice without prior adaptation. In addition, in guinea pig model, HB93 was transmissible by direct contact, but HB95 was transmissible via respiratory droplets. These results revealed the potential threat to public health posed by H7N6 influenza viruses and emphasized the need for continued surveillance of the circulation of this subtype in poultry. |
topic |
avian H7N6 influenza A virus reassortment receptor binding pathogenicity transmissibility |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2019.00018/full |
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