ISOLATION AND CHARACTERIZATION OF INSULIN RECEPTOR OF PLASMA MEMBRANES OF RAT LIVER CELLS AT MODEL OF TYPE 2 DIABETES

• Main Authors: T. I. Halenova, M. Y. Kuznetsova, O. M. Savchuk, L. I. Ostapchenko
• Format: Article
• Language: English
• Published: National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine. 2014-06-01
• Series: Biotechnologia Acta
• Subjects: insulin receptor; insulin resistance; type 2 diabetes mellitus
• Online Access: http://biotechnology.kiev.ua/images/storage/3_2014/galenova.pdf

The insulin receptor was isolated from the liver cell membranes of control and diabetic rats. The protein purity was controlled by electrophoresis and Western blot. The tyrosine kinase activity of the insulin receptor was investigated in the incubation medium in the presence of insulin (concentration range: from 10-11 M to 10-5 M). The basal tyrosine kinase activity of the insulin receptor (in the absence of insulin) was equal for the control and diabetic state. Maximal tyrosine kinase activity of the diabetic and control insulin receptor was observed at the insulin concentration of 10-8 M. Results indicate that type 2 diabetes did not cause the irregularities in the functioning of the insulin receptor that could be the reason of insulin resistance.