ISOLATION AND CHARACTERIZATION OF INSULIN RECEPTOR OF PLASMA MEMBRANES OF RAT LIVER CELLS AT MODEL OF TYPE 2 DIABETES
The insulin receptor was isolated from the liver cell membranes of control and diabetic rats. The protein purity was controlled by electrophoresis and Western blot. The tyrosine kinase activity of the insulin receptor was investigated in the incubation medium in the presence of insulin (concentratio...
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National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine.
2014-06-01
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doaj-86855ab9ebaa482ab50d9df1129216a92020-11-24T23:23:20ZengNational Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine.Biotechnologia Acta2410-77512410-776X2014-06-0173818710.15407/biotech7.03.081ISOLATION AND CHARACTERIZATION OF INSULIN RECEPTOR OF PLASMA MEMBRANES OF RAT LIVER CELLS AT MODEL OF TYPE 2 DIABETEST. I. Halenova0M. Y. Kuznetsova1 O. M. Savchuk2L. I. Ostapchenko3Taras Shevchenko National University of Kyiv, UkraineTaras Shevchenko National University of Kyiv, UkraineTaras Shevchenko National University of Kyiv, UkraineTaras Shevchenko National University of Kyiv, UkraineThe insulin receptor was isolated from the liver cell membranes of control and diabetic rats. The protein purity was controlled by electrophoresis and Western blot. The tyrosine kinase activity of the insulin receptor was investigated in the incubation medium in the presence of insulin (concentration range: from 10-11 M to 10-5 M). The basal tyrosine kinase activity of the insulin receptor (in the absence of insulin) was equal for the control and diabetic state. Maximal tyrosine kinase activity of the diabetic and control insulin receptor was observed at the insulin concentration of 10-8 M. Results indicate that type 2 diabetes did not cause the irregularities in the functioning of the insulin receptor that could be the reason of insulin resistance.http://biotechnology.kiev.ua/images/storage/3_2014/galenova.pdfinsulin receptorinsulin resistancetype 2 diabetes mellitus |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
T. I. Halenova M. Y. Kuznetsova O. M. Savchuk L. I. Ostapchenko |
spellingShingle |
T. I. Halenova M. Y. Kuznetsova O. M. Savchuk L. I. Ostapchenko ISOLATION AND CHARACTERIZATION OF INSULIN RECEPTOR OF PLASMA MEMBRANES OF RAT LIVER CELLS AT MODEL OF TYPE 2 DIABETES Biotechnologia Acta insulin receptor insulin resistance type 2 diabetes mellitus |
author_facet |
T. I. Halenova M. Y. Kuznetsova O. M. Savchuk L. I. Ostapchenko |
author_sort |
T. I. Halenova |
title |
ISOLATION AND CHARACTERIZATION OF INSULIN RECEPTOR OF PLASMA MEMBRANES OF RAT LIVER CELLS AT MODEL OF TYPE 2 DIABETES |
title_short |
ISOLATION AND CHARACTERIZATION OF INSULIN RECEPTOR OF PLASMA MEMBRANES OF RAT LIVER CELLS AT MODEL OF TYPE 2 DIABETES |
title_full |
ISOLATION AND CHARACTERIZATION OF INSULIN RECEPTOR OF PLASMA MEMBRANES OF RAT LIVER CELLS AT MODEL OF TYPE 2 DIABETES |
title_fullStr |
ISOLATION AND CHARACTERIZATION OF INSULIN RECEPTOR OF PLASMA MEMBRANES OF RAT LIVER CELLS AT MODEL OF TYPE 2 DIABETES |
title_full_unstemmed |
ISOLATION AND CHARACTERIZATION OF INSULIN RECEPTOR OF PLASMA MEMBRANES OF RAT LIVER CELLS AT MODEL OF TYPE 2 DIABETES |
title_sort |
isolation and characterization of insulin receptor of plasma membranes of rat liver cells at model of type 2 diabetes |
publisher |
National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine. |
series |
Biotechnologia Acta |
issn |
2410-7751 2410-776X |
publishDate |
2014-06-01 |
description |
The insulin receptor was isolated from the liver cell membranes of control and diabetic rats. The protein purity was controlled by electrophoresis and Western blot. The tyrosine kinase activity of the insulin receptor was investigated in the incubation medium in the presence of insulin (concentration range: from 10-11 M to 10-5 M). The basal tyrosine kinase activity of the insulin receptor (in the absence of insulin) was equal for the control and diabetic state. Maximal tyrosine kinase activity of the diabetic and control insulin receptor was observed at the insulin concentration of 10-8 M. Results indicate that type 2 diabetes did not cause the irregularities in the functioning of the insulin receptor that could be the reason of insulin resistance. |
topic |
insulin receptor insulin resistance type 2 diabetes mellitus |
url |
http://biotechnology.kiev.ua/images/storage/3_2014/galenova.pdf |
work_keys_str_mv |
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