Effects of Aged Oil Sludge on Soil Physicochemical Properties and Fungal Diversity Revealed by High-Throughput Sequencing Analysis
The oilfield soil was contaminated for years by large quantities of aged oil sludge generated in the petroleum industry. In this study, physicochemical properties, contents of main pollutants, and fungal diversity of the aged oil sludge-contaminated soil were analyzed. Results revealed that aged oil...
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doaj-86a65f1971e54583b17e55afee2d2e3a2021-07-02T16:51:34ZengHindawi LimitedArchaea1472-36461472-36542018-01-01201810.1155/2018/92642599264259Effects of Aged Oil Sludge on Soil Physicochemical Properties and Fungal Diversity Revealed by High-Throughput Sequencing AnalysisHuihui Wang0Shaoping Kuang1Qiaolin Lang2Wenjuan Yu3College of Environment and Safety Engineering, Qingdao University of Science and Technology, Qingdao, 266042 Shandong Province, ChinaCollege of Environment and Safety Engineering, Qingdao University of Science and Technology, Qingdao, 266042 Shandong Province, ChinaCollege of Environment and Safety Engineering, Qingdao University of Science and Technology, Qingdao, 266042 Shandong Province, ChinaCollege of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042 Shandong Province, ChinaThe oilfield soil was contaminated for years by large quantities of aged oil sludge generated in the petroleum industry. In this study, physicochemical properties, contents of main pollutants, and fungal diversity of the aged oil sludge-contaminated soil were analyzed. Results revealed that aged oil sludge significantly changed physical and chemical properties of the receiving soil and increased the contents of main pollutants (petroleum hydrocarbons and heavy metals) in soil. Meanwhile, the internal transcribed spacer (ITS) sequencing by Illumina Miseq platform at each taxonomic level demonstrated that the toxicological effect of oil pollutants obviously influenced the fungal diversity and community structure in soil. Moreover, it was found that the presence of three genera (Cephalotheca, Lecanicillium, and Septoriella) appeared in aged oil sludge-contaminated soil. And oil pollutants promoted the growth of certain genera in Ascomycota (70.83%) and Basidiomycota (10.78%), such as Venturia, Alternaria, and Piloderma. Nevertheless, the growth of Mortierella (9.16%), Emericella (6.02%), and Bjerkandera (0.00%) was intensively limited. This study would aid thorough understanding of microbial diversity in oil-contaminated soil and thus provide new point of view to soil bioremediation.http://dx.doi.org/10.1155/2018/9264259 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Huihui Wang Shaoping Kuang Qiaolin Lang Wenjuan Yu |
spellingShingle |
Huihui Wang Shaoping Kuang Qiaolin Lang Wenjuan Yu Effects of Aged Oil Sludge on Soil Physicochemical Properties and Fungal Diversity Revealed by High-Throughput Sequencing Analysis Archaea |
author_facet |
Huihui Wang Shaoping Kuang Qiaolin Lang Wenjuan Yu |
author_sort |
Huihui Wang |
title |
Effects of Aged Oil Sludge on Soil Physicochemical Properties and Fungal Diversity Revealed by High-Throughput Sequencing Analysis |
title_short |
Effects of Aged Oil Sludge on Soil Physicochemical Properties and Fungal Diversity Revealed by High-Throughput Sequencing Analysis |
title_full |
Effects of Aged Oil Sludge on Soil Physicochemical Properties and Fungal Diversity Revealed by High-Throughput Sequencing Analysis |
title_fullStr |
Effects of Aged Oil Sludge on Soil Physicochemical Properties and Fungal Diversity Revealed by High-Throughput Sequencing Analysis |
title_full_unstemmed |
Effects of Aged Oil Sludge on Soil Physicochemical Properties and Fungal Diversity Revealed by High-Throughput Sequencing Analysis |
title_sort |
effects of aged oil sludge on soil physicochemical properties and fungal diversity revealed by high-throughput sequencing analysis |
publisher |
Hindawi Limited |
series |
Archaea |
issn |
1472-3646 1472-3654 |
publishDate |
2018-01-01 |
description |
The oilfield soil was contaminated for years by large quantities of aged oil sludge generated in the petroleum industry. In this study, physicochemical properties, contents of main pollutants, and fungal diversity of the aged oil sludge-contaminated soil were analyzed. Results revealed that aged oil sludge significantly changed physical and chemical properties of the receiving soil and increased the contents of main pollutants (petroleum hydrocarbons and heavy metals) in soil. Meanwhile, the internal transcribed spacer (ITS) sequencing by Illumina Miseq platform at each taxonomic level demonstrated that the toxicological effect of oil pollutants obviously influenced the fungal diversity and community structure in soil. Moreover, it was found that the presence of three genera (Cephalotheca, Lecanicillium, and Septoriella) appeared in aged oil sludge-contaminated soil. And oil pollutants promoted the growth of certain genera in Ascomycota (70.83%) and Basidiomycota (10.78%), such as Venturia, Alternaria, and Piloderma. Nevertheless, the growth of Mortierella (9.16%), Emericella (6.02%), and Bjerkandera (0.00%) was intensively limited. This study would aid thorough understanding of microbial diversity in oil-contaminated soil and thus provide new point of view to soil bioremediation. |
url |
http://dx.doi.org/10.1155/2018/9264259 |
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