Immunomodulatory Activities of Sambucus javanica Extracts in DMBAExposed BALB/c Mouse
Purpose: Accumulating evidence shows the genus of Sambucus exerts a broad spectrum of medicinal potencies such as anticancer, antiviral, antibacterial, and antidiabetes. Based on the previous studies, we hypothesized that bioactive compounds of Sambucus might alter several biological systems, includ...
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Tabriz University of Medical Sciences
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doaj-86b8f5236a0b4007b46fb29308475b322020-11-25T02:17:54ZengTabriz University of Medical Sciences Advanced Pharmaceutical Bulletin2228-58812251-73082019-10-019461962310.15171/apb.2019.071apb-27480Immunomodulatory Activities of Sambucus javanica Extracts in DMBAExposed BALB/c MouseWira Eka Putra0Muhaimin Rifa'i1Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Indonesia.Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Indonesia.Purpose: Accumulating evidence shows the genus of Sambucus exerts a broad spectrum of medicinal potencies such as anticancer, antiviral, antibacterial, and antidiabetes. Based on the previous studies, we hypothesized that bioactive compounds of Sambucus might alter several biological systems, including the immune system. Therefore, this study extensively aimed to evaluate the immunomodulatory activities of Sambucus javanica extracts in 7,12-dimethylbenz[a] anthracene (DMBA)-treated BALB/c mouse. Methods: The experimental mice were orally administrated with 2.8 mg.kg-1 BW of DMBA for ten times within a month. After that, the mice were treated by S. javanica berries and leaves extracts for 2 weeks. Subsequently, the inflammation rate was evaluated by using flow cytometry analysis, whereas the necrosis incidences were observed by hematoxylin & eosin staining. Results: Based on the results, we found the expression of tumor necrosis factor alpha (TNF-α) and interferon gamma (IFN-ɣ) were increased however after treated by S. javanica berries and leaves extracts were significantly decreased. In the same way, necrosis incidence was increased in the DMBA-treated group however it was diminished with S. javanica extracts treatment. Conclusion: Together, these results suggested that S. javanica extracts have immunomodulatory activities to suppress inflammation and reduce necrosis incidence in experimental mice. <br />https://apb.tbzmed.ac.ir/PDF/apb-27480dmbabalb/c miceimmunomodulationinflammationsambucus javanica |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wira Eka Putra Muhaimin Rifa'i |
spellingShingle |
Wira Eka Putra Muhaimin Rifa'i Immunomodulatory Activities of Sambucus javanica Extracts in DMBAExposed BALB/c Mouse Advanced Pharmaceutical Bulletin dmba balb/c mice immunomodulation inflammation sambucus javanica |
author_facet |
Wira Eka Putra Muhaimin Rifa'i |
author_sort |
Wira Eka Putra |
title |
Immunomodulatory Activities of Sambucus javanica Extracts in DMBAExposed BALB/c Mouse |
title_short |
Immunomodulatory Activities of Sambucus javanica Extracts in DMBAExposed BALB/c Mouse |
title_full |
Immunomodulatory Activities of Sambucus javanica Extracts in DMBAExposed BALB/c Mouse |
title_fullStr |
Immunomodulatory Activities of Sambucus javanica Extracts in DMBAExposed BALB/c Mouse |
title_full_unstemmed |
Immunomodulatory Activities of Sambucus javanica Extracts in DMBAExposed BALB/c Mouse |
title_sort |
immunomodulatory activities of sambucus javanica extracts in dmbaexposed balb/c mouse |
publisher |
Tabriz University of Medical Sciences |
series |
Advanced Pharmaceutical Bulletin |
issn |
2228-5881 2251-7308 |
publishDate |
2019-10-01 |
description |
Purpose: Accumulating evidence shows the genus of Sambucus exerts a broad spectrum of medicinal potencies such as anticancer, antiviral, antibacterial, and antidiabetes. Based on the previous studies, we hypothesized that bioactive compounds of Sambucus might alter several biological systems, including the immune system. Therefore, this study extensively aimed to evaluate the immunomodulatory activities of Sambucus javanica extracts in 7,12-dimethylbenz[a] anthracene (DMBA)-treated BALB/c mouse. Methods: The experimental mice were orally administrated with 2.8 mg.kg-1 BW of DMBA for ten times within a month. After that, the mice were treated by S. javanica berries and leaves extracts for 2 weeks. Subsequently, the inflammation rate was evaluated by using flow cytometry analysis, whereas the necrosis incidences were observed by hematoxylin & eosin staining. Results: Based on the results, we found the expression of tumor necrosis factor alpha (TNF-α) and interferon gamma (IFN-ɣ) were increased however after treated by S. javanica berries and leaves extracts were significantly decreased. In the same way, necrosis incidence was increased in the DMBA-treated group however it was diminished with S. javanica extracts treatment. Conclusion: Together, these results suggested that S. javanica extracts have immunomodulatory activities to suppress inflammation and reduce necrosis incidence in experimental mice. <br /> |
topic |
dmba balb/c mice immunomodulation inflammation sambucus javanica |
url |
https://apb.tbzmed.ac.ir/PDF/apb-27480 |
work_keys_str_mv |
AT wiraekaputra immunomodulatoryactivitiesofsambucusjavanicaextractsindmbaexposedbalbcmouse AT muhaiminrifai immunomodulatoryactivitiesofsambucusjavanicaextractsindmbaexposedbalbcmouse |
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