Detection of human disease conditions by single-cell morpho-rheological phenotyping of blood
Blood is arguably the most important bodily fluid and its analysis provides crucial health status information. A first routine measure to narrow down diagnosis in clinical practice is the differential blood count, determining the frequency of all major blood cells. What is lacking to advance initial...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2018-01-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/29213 |
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doaj-86b96256276f42619ec6a394f2c8b4ff |
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| record_format |
Article |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Nicole Toepfner Christoph Herold Oliver Otto Philipp Rosendahl Angela Jacobi Martin Kräter Julia Stächele Leonhard Menschner Maik Herbig Laura Ciuffreda Lisa Ranford-Cartwright Michal Grzybek Ünal Coskun Elisabeth Reithuber Geneviève Garriss Peter Mellroth Birgitta Henriques-Normark Nicola Tregay Meinolf Suttorp Martin Bornhäuser Edwin R Chilvers Reinhard Berner Jochen Guck |
| spellingShingle |
Nicole Toepfner Christoph Herold Oliver Otto Philipp Rosendahl Angela Jacobi Martin Kräter Julia Stächele Leonhard Menschner Maik Herbig Laura Ciuffreda Lisa Ranford-Cartwright Michal Grzybek Ünal Coskun Elisabeth Reithuber Geneviève Garriss Peter Mellroth Birgitta Henriques-Normark Nicola Tregay Meinolf Suttorp Martin Bornhäuser Edwin R Chilvers Reinhard Berner Jochen Guck Detection of human disease conditions by single-cell morpho-rheological phenotyping of blood eLife real-time deformability cytometry cell mechanics spherocytosis malaria neutrophil activation leukemia |
| author_facet |
Nicole Toepfner Christoph Herold Oliver Otto Philipp Rosendahl Angela Jacobi Martin Kräter Julia Stächele Leonhard Menschner Maik Herbig Laura Ciuffreda Lisa Ranford-Cartwright Michal Grzybek Ünal Coskun Elisabeth Reithuber Geneviève Garriss Peter Mellroth Birgitta Henriques-Normark Nicola Tregay Meinolf Suttorp Martin Bornhäuser Edwin R Chilvers Reinhard Berner Jochen Guck |
| author_sort |
Nicole Toepfner |
| title |
Detection of human disease conditions by single-cell morpho-rheological phenotyping of blood |
| title_short |
Detection of human disease conditions by single-cell morpho-rheological phenotyping of blood |
| title_full |
Detection of human disease conditions by single-cell morpho-rheological phenotyping of blood |
| title_fullStr |
Detection of human disease conditions by single-cell morpho-rheological phenotyping of blood |
| title_full_unstemmed |
Detection of human disease conditions by single-cell morpho-rheological phenotyping of blood |
| title_sort |
detection of human disease conditions by single-cell morpho-rheological phenotyping of blood |
| publisher |
eLife Sciences Publications Ltd |
| series |
eLife |
| issn |
2050-084X |
| publishDate |
2018-01-01 |
| description |
Blood is arguably the most important bodily fluid and its analysis provides crucial health status information. A first routine measure to narrow down diagnosis in clinical practice is the differential blood count, determining the frequency of all major blood cells. What is lacking to advance initial blood diagnostics is an unbiased and quick functional assessment of blood that can narrow down the diagnosis and generate specific hypotheses. To address this need, we introduce the continuous, cell-by-cell morpho-rheological (MORE) analysis of diluted whole blood, without labeling, enrichment or separation, at rates of 1000 cells/sec. In a drop of blood we can identify all major blood cells and characterize their pathological changes in several disease conditions in vitro and in patient samples. This approach takes previous results of mechanical studies on specifically isolated blood cells to the level of application directly in blood and adds a functional dimension to conventional blood analysis. |
| topic |
real-time deformability cytometry cell mechanics spherocytosis malaria neutrophil activation leukemia |
| url |
https://elifesciences.org/articles/29213 |
| work_keys_str_mv |
AT nicoletoepfner detectionofhumandiseaseconditionsbysinglecellmorphorheologicalphenotypingofblood AT christophherold detectionofhumandiseaseconditionsbysinglecellmorphorheologicalphenotypingofblood AT oliverotto detectionofhumandiseaseconditionsbysinglecellmorphorheologicalphenotypingofblood AT philipprosendahl detectionofhumandiseaseconditionsbysinglecellmorphorheologicalphenotypingofblood AT angelajacobi detectionofhumandiseaseconditionsbysinglecellmorphorheologicalphenotypingofblood AT martinkrater detectionofhumandiseaseconditionsbysinglecellmorphorheologicalphenotypingofblood AT juliastachele detectionofhumandiseaseconditionsbysinglecellmorphorheologicalphenotypingofblood AT leonhardmenschner detectionofhumandiseaseconditionsbysinglecellmorphorheologicalphenotypingofblood AT maikherbig detectionofhumandiseaseconditionsbysinglecellmorphorheologicalphenotypingofblood AT lauraciuffreda detectionofhumandiseaseconditionsbysinglecellmorphorheologicalphenotypingofblood AT lisaranfordcartwright detectionofhumandiseaseconditionsbysinglecellmorphorheologicalphenotypingofblood AT michalgrzybek detectionofhumandiseaseconditionsbysinglecellmorphorheologicalphenotypingofblood AT unalcoskun detectionofhumandiseaseconditionsbysinglecellmorphorheologicalphenotypingofblood AT elisabethreithuber detectionofhumandiseaseconditionsbysinglecellmorphorheologicalphenotypingofblood AT genevievegarriss detectionofhumandiseaseconditionsbysinglecellmorphorheologicalphenotypingofblood AT petermellroth detectionofhumandiseaseconditionsbysinglecellmorphorheologicalphenotypingofblood AT birgittahenriquesnormark detectionofhumandiseaseconditionsbysinglecellmorphorheologicalphenotypingofblood AT nicolatregay detectionofhumandiseaseconditionsbysinglecellmorphorheologicalphenotypingofblood AT meinolfsuttorp detectionofhumandiseaseconditionsbysinglecellmorphorheologicalphenotypingofblood AT martinbornhauser detectionofhumandiseaseconditionsbysinglecellmorphorheologicalphenotypingofblood AT edwinrchilvers detectionofhumandiseaseconditionsbysinglecellmorphorheologicalphenotypingofblood AT reinhardberner detectionofhumandiseaseconditionsbysinglecellmorphorheologicalphenotypingofblood AT jochenguck detectionofhumandiseaseconditionsbysinglecellmorphorheologicalphenotypingofblood |
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1721459917791952896 |
| spelling |
doaj-86b96256276f42619ec6a394f2c8b4ff2021-05-05T15:30:57ZengeLife Sciences Publications LtdeLife2050-084X2018-01-01710.7554/eLife.29213Detection of human disease conditions by single-cell morpho-rheological phenotyping of bloodNicole Toepfner0Christoph Herold1Oliver Otto2Philipp Rosendahl3https://orcid.org/0000-0002-9545-5045Angela Jacobi4Martin Kräter5https://orcid.org/0000-0001-7122-7331Julia Stächele6Leonhard Menschner7Maik Herbig8Laura Ciuffreda9Lisa Ranford-Cartwright10Michal Grzybek11Ünal Coskun12https://orcid.org/0000-0003-4375-3144Elisabeth Reithuber13Geneviève Garriss14https://orcid.org/0000-0002-5361-0975Peter Mellroth15Birgitta Henriques-Normark16Nicola Tregay17Meinolf Suttorp18Martin Bornhäuser19Edwin R Chilvers20https://orcid.org/0000-0002-4230-9677Reinhard Berner21Jochen Guck22https://orcid.org/0000-0002-1453-6119Center of Molecular and Cellular Bioengineering, Biotechnology Center, Technische Universität Dresden, Dresden, Germany; Department of Medicine, University of Cambridge, Cambridge, United Kingdom; Department of Pediatrics, University Clinic Carl Gustav Carus, Technische Universität Dresden, Dresden, GermanyCenter of Molecular and Cellular Bioengineering, Biotechnology Center, Technische Universität Dresden, Dresden, Germany; Zellmechanik Dresden GmbH, Dresden, GermanyCenter of Molecular and Cellular Bioengineering, Biotechnology Center, Technische Universität Dresden, Dresden, Germany; Zellmechanik Dresden GmbH, Dresden, Germany; ZIK HIKE, Universität Greifswald, Greifswald, GermanyCenter of Molecular and Cellular Bioengineering, Biotechnology Center, Technische Universität Dresden, Dresden, Germany; Zellmechanik Dresden GmbH, Dresden, GermanyCenter of Molecular and Cellular Bioengineering, Biotechnology Center, Technische Universität Dresden, Dresden, GermanyDepartment of Hematology and Oncology, University Clinic Carl Gustav Carus, Technische Universität Dresden, Dresden, GermanyDepartment of Pediatrics, University Clinic Carl Gustav Carus, Technische Universität Dresden, Dresden, GermanyDepartment of Pediatrics, University Clinic Carl Gustav Carus, Technische Universität Dresden, Dresden, GermanyCenter of Molecular and Cellular Bioengineering, Biotechnology Center, Technische Universität Dresden, Dresden, GermanyInstitute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow, United KingdomInstitute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow, United KingdomPaul Langerhans Institute Dresden of the Helmholtz Centre Munich, University Hospital and Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany; German Center for Diabetes Research, Neuherberg, GermanyPaul Langerhans Institute Dresden of the Helmholtz Centre Munich, University Hospital and Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany; German Center for Diabetes Research, Neuherberg, GermanyDepartment of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden; Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, SwedenDepartment of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden; Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, SwedenDepartment of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden; Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, SwedenDepartment of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden; Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, SwedenDepartment of Medicine, University of Cambridge, Cambridge, United KingdomDepartment of Pediatrics, University Clinic Carl Gustav Carus, Technische Universität Dresden, Dresden, GermanyDepartment of Hematology and Oncology, University Clinic Carl Gustav Carus, Technische Universität Dresden, Dresden, GermanyDepartment of Medicine, University of Cambridge, Cambridge, United KingdomDepartment of Pediatrics, University Clinic Carl Gustav Carus, Technische Universität Dresden, Dresden, GermanyCenter of Molecular and Cellular Bioengineering, Biotechnology Center, Technische Universität Dresden, Dresden, GermanyBlood is arguably the most important bodily fluid and its analysis provides crucial health status information. A first routine measure to narrow down diagnosis in clinical practice is the differential blood count, determining the frequency of all major blood cells. What is lacking to advance initial blood diagnostics is an unbiased and quick functional assessment of blood that can narrow down the diagnosis and generate specific hypotheses. To address this need, we introduce the continuous, cell-by-cell morpho-rheological (MORE) analysis of diluted whole blood, without labeling, enrichment or separation, at rates of 1000 cells/sec. In a drop of blood we can identify all major blood cells and characterize their pathological changes in several disease conditions in vitro and in patient samples. This approach takes previous results of mechanical studies on specifically isolated blood cells to the level of application directly in blood and adds a functional dimension to conventional blood analysis.https://elifesciences.org/articles/29213real-time deformability cytometrycell mechanicsspherocytosismalarianeutrophil activationleukemia |