Application of atomic force microscopy in cancer research
Abstract Atomic force microscopy (AFM) allows for nanometer-scale investigation of cells and molecules. Recent advances have enabled its application in cancer research and diagnosis. The physicochemical properties of live cells undergo changes when their physiological conditions are altered. These p...
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Format: | Article |
Language: | English |
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BMC
2018-12-01
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Series: | Journal of Nanobiotechnology |
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Online Access: | http://link.springer.com/article/10.1186/s12951-018-0428-0 |
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doaj-86e1d65dcf1f4f449792d1c4ff5974a2 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiangying Deng Fang Xiong Xiayu Li Bo Xiang Zheng Li Xu Wu Can Guo Xiaoling Li Yong Li Guiyuan Li Wei Xiong Zhaoyang Zeng |
spellingShingle |
Xiangying Deng Fang Xiong Xiayu Li Bo Xiang Zheng Li Xu Wu Can Guo Xiaoling Li Yong Li Guiyuan Li Wei Xiong Zhaoyang Zeng Application of atomic force microscopy in cancer research Journal of Nanobiotechnology Atomic force microscopy Cancer cells Morphology Mechanical properties |
author_facet |
Xiangying Deng Fang Xiong Xiayu Li Bo Xiang Zheng Li Xu Wu Can Guo Xiaoling Li Yong Li Guiyuan Li Wei Xiong Zhaoyang Zeng |
author_sort |
Xiangying Deng |
title |
Application of atomic force microscopy in cancer research |
title_short |
Application of atomic force microscopy in cancer research |
title_full |
Application of atomic force microscopy in cancer research |
title_fullStr |
Application of atomic force microscopy in cancer research |
title_full_unstemmed |
Application of atomic force microscopy in cancer research |
title_sort |
application of atomic force microscopy in cancer research |
publisher |
BMC |
series |
Journal of Nanobiotechnology |
issn |
1477-3155 |
publishDate |
2018-12-01 |
description |
Abstract Atomic force microscopy (AFM) allows for nanometer-scale investigation of cells and molecules. Recent advances have enabled its application in cancer research and diagnosis. The physicochemical properties of live cells undergo changes when their physiological conditions are altered. These physicochemical properties can therefore reflect complex physiological processes occurring in cells. When cells are in the process of carcinogenesis and stimulated by external stimuli, their morphology, elasticity, and adhesion properties may change. AFM can perform surface imaging and ultrastructural observation of live cells with atomic resolution under near-physiological conditions, collecting force spectroscopy information which allows for the study of the mechanical properties of cells. For this reason, AFM has potential to be used as a tool for high resolution research into the ultrastructure and mechanical properties of tumor cells. This review describes the working principle, working mode, and technical points of atomic force microscopy, and reviews the applications and prospects of atomic force microscopy in cancer research. |
topic |
Atomic force microscopy Cancer cells Morphology Mechanical properties |
url |
http://link.springer.com/article/10.1186/s12951-018-0428-0 |
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1725182884868784128 |
spelling |
doaj-86e1d65dcf1f4f449792d1c4ff5974a22020-11-25T01:08:23ZengBMCJournal of Nanobiotechnology1477-31552018-12-0116111510.1186/s12951-018-0428-0Application of atomic force microscopy in cancer researchXiangying Deng0Fang Xiong1Xiayu Li2Bo Xiang3Zheng Li4Xu Wu5Can Guo6Xiaoling Li7Yong Li8Guiyuan Li9Wei Xiong10Zhaoyang Zeng11The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health and Hunan Key Laboratory of Translational Radiation Oncology, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityThe Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medical Science, Xiangya School of Medicine, Central South UniversityThe Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medical Science, Xiangya School of Medicine, Central South UniversityThe Key Laboratory of Carcinogenesis of the Chinese Ministry of Health and Hunan Key Laboratory of Translational Radiation Oncology, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityThe Key Laboratory of Carcinogenesis of the Chinese Ministry of Health and Hunan Key Laboratory of Translational Radiation Oncology, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityThe Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medical Science, Xiangya School of Medicine, Central South UniversityThe Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medical Science, Xiangya School of Medicine, Central South UniversityThe Key Laboratory of Carcinogenesis of the Chinese Ministry of Health and Hunan Key Laboratory of Translational Radiation Oncology, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityThe Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medical Science, Xiangya School of Medicine, Central South UniversityThe Key Laboratory of Carcinogenesis of the Chinese Ministry of Health and Hunan Key Laboratory of Translational Radiation Oncology, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityThe Key Laboratory of Carcinogenesis of the Chinese Ministry of Health and Hunan Key Laboratory of Translational Radiation Oncology, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityThe Key Laboratory of Carcinogenesis of the Chinese Ministry of Health and Hunan Key Laboratory of Translational Radiation Oncology, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityAbstract Atomic force microscopy (AFM) allows for nanometer-scale investigation of cells and molecules. Recent advances have enabled its application in cancer research and diagnosis. The physicochemical properties of live cells undergo changes when their physiological conditions are altered. These physicochemical properties can therefore reflect complex physiological processes occurring in cells. When cells are in the process of carcinogenesis and stimulated by external stimuli, their morphology, elasticity, and adhesion properties may change. AFM can perform surface imaging and ultrastructural observation of live cells with atomic resolution under near-physiological conditions, collecting force spectroscopy information which allows for the study of the mechanical properties of cells. For this reason, AFM has potential to be used as a tool for high resolution research into the ultrastructure and mechanical properties of tumor cells. This review describes the working principle, working mode, and technical points of atomic force microscopy, and reviews the applications and prospects of atomic force microscopy in cancer research.http://link.springer.com/article/10.1186/s12951-018-0428-0Atomic force microscopyCancer cellsMorphologyMechanical properties |