Preparation and Chromatographic Application of β-Cyclodextrin Molecularly Imprinted Microspheres for Paeoniflorin
The application of molecular imprinting technology in the separation and purification of active ingredients in natural products was widely reported, but remains a challenge. Enrichment and separation are especially limited. A surface imprinting technique was reported to synthesize molecularly imprin...
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doaj-86ea8c65a9cc4ff6805501d6e0a1b5dd2020-11-24T20:55:11ZengMDPI AGPolymers2073-43602017-06-019621410.3390/polym9060214polym9060214Preparation and Chromatographic Application of β-Cyclodextrin Molecularly Imprinted Microspheres for PaeoniflorinWei Zhang0Bofeng Wei1Shoujiang Li2Yueming Wang3Shaoyan Wang4School of Chemical Engineering, University of Science and Technology, Anshan 114051, Liaoning, ChinaLiaoning Provincial Key Laboratory of Fine Separation Technique, University of Science and Technology, Anshan 114051, Liaoning, ChinaLiaoning Provincial Key Laboratory of Fine Separation Technique, University of Science and Technology, Anshan 114051, Liaoning, ChinaLiaoning Provincial Key Laboratory of Fine Separation Technique, University of Science and Technology, Anshan 114051, Liaoning, ChinaSchool of Chemical Engineering, University of Science and Technology, Anshan 114051, Liaoning, ChinaThe application of molecular imprinting technology in the separation and purification of active ingredients in natural products was widely reported, but remains a challenge. Enrichment and separation are especially limited. A surface imprinting technique was reported to synthesize molecularly imprinted microspheres (MIMs) in this article. With paeoniflorin (PF) as the template molecule, β-cyclodextrin (β-CD) and acrylamide (AA) as the functional monomers, and poly(glycidyl methacrylate, GMA) microspheres (PGMA) as the backing material. MIMs have been characterized by FTIR and FESEM. Adsorption experiments indicated the adsorption capacity of MIMs was superior to those comparative non-imprinted microspheres (NIMs) and the binding isotherm of MIMs was in good agreement with the two-site binding model. The baseline separation of PF and its structural analogue albiflorin (AF) were achieved on the new MIMs packed column. MIMs showed good affinity and efficiency for separation of PF and AF compared with those comparative NIMs. The approach of fabricating MIMs is simple, rapid, and inexpensive, and may shed new light on the application of MIMs as a liquid chromatography stationary phase to separate and analyze PF and AF from the Red peony root extracts.http://www.mdpi.com/2073-4360/9/6/214molecular imprintingstationary phasepaeoniflorinchromatographic application |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wei Zhang Bofeng Wei Shoujiang Li Yueming Wang Shaoyan Wang |
spellingShingle |
Wei Zhang Bofeng Wei Shoujiang Li Yueming Wang Shaoyan Wang Preparation and Chromatographic Application of β-Cyclodextrin Molecularly Imprinted Microspheres for Paeoniflorin Polymers molecular imprinting stationary phase paeoniflorin chromatographic application |
author_facet |
Wei Zhang Bofeng Wei Shoujiang Li Yueming Wang Shaoyan Wang |
author_sort |
Wei Zhang |
title |
Preparation and Chromatographic Application of β-Cyclodextrin Molecularly Imprinted Microspheres for Paeoniflorin |
title_short |
Preparation and Chromatographic Application of β-Cyclodextrin Molecularly Imprinted Microspheres for Paeoniflorin |
title_full |
Preparation and Chromatographic Application of β-Cyclodextrin Molecularly Imprinted Microspheres for Paeoniflorin |
title_fullStr |
Preparation and Chromatographic Application of β-Cyclodextrin Molecularly Imprinted Microspheres for Paeoniflorin |
title_full_unstemmed |
Preparation and Chromatographic Application of β-Cyclodextrin Molecularly Imprinted Microspheres for Paeoniflorin |
title_sort |
preparation and chromatographic application of β-cyclodextrin molecularly imprinted microspheres for paeoniflorin |
publisher |
MDPI AG |
series |
Polymers |
issn |
2073-4360 |
publishDate |
2017-06-01 |
description |
The application of molecular imprinting technology in the separation and purification of active ingredients in natural products was widely reported, but remains a challenge. Enrichment and separation are especially limited. A surface imprinting technique was reported to synthesize molecularly imprinted microspheres (MIMs) in this article. With paeoniflorin (PF) as the template molecule, β-cyclodextrin (β-CD) and acrylamide (AA) as the functional monomers, and poly(glycidyl methacrylate, GMA) microspheres (PGMA) as the backing material. MIMs have been characterized by FTIR and FESEM. Adsorption experiments indicated the adsorption capacity of MIMs was superior to those comparative non-imprinted microspheres (NIMs) and the binding isotherm of MIMs was in good agreement with the two-site binding model. The baseline separation of PF and its structural analogue albiflorin (AF) were achieved on the new MIMs packed column. MIMs showed good affinity and efficiency for separation of PF and AF compared with those comparative NIMs. The approach of fabricating MIMs is simple, rapid, and inexpensive, and may shed new light on the application of MIMs as a liquid chromatography stationary phase to separate and analyze PF and AF from the Red peony root extracts. |
topic |
molecular imprinting stationary phase paeoniflorin chromatographic application |
url |
http://www.mdpi.com/2073-4360/9/6/214 |
work_keys_str_mv |
AT weizhang preparationandchromatographicapplicationofbcyclodextrinmolecularlyimprintedmicrospheresforpaeoniflorin AT bofengwei preparationandchromatographicapplicationofbcyclodextrinmolecularlyimprintedmicrospheresforpaeoniflorin AT shoujiangli preparationandchromatographicapplicationofbcyclodextrinmolecularlyimprintedmicrospheresforpaeoniflorin AT yuemingwang preparationandchromatographicapplicationofbcyclodextrinmolecularlyimprintedmicrospheresforpaeoniflorin AT shaoyanwang preparationandchromatographicapplicationofbcyclodextrinmolecularlyimprintedmicrospheresforpaeoniflorin |
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1716792267195285504 |